İneklerde Subklinik Mastitis Tanısında Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin Değerlendirilmesi

Subklinik mastitis, en yaygın gözlenen önemli yangısal sürü hastalıklarından birisidir. Çalışmamızda bu hastalığın tanısında süt ve kanda amiloid A (AA) ve C-reaktif protein (CRP) parametrelerinin yeni bir tanı seçeneği olarak ortaya konulması amaçlanmıştır. Çalışma laktasyonun farklı dönemlerinde bulunan toplam 40 adet Holstein ırkı sağmal inek üzerinde yürütüldü. Strip kap muayenesinin ardından süt ve meme muayenesinde herhangi bir fiziksel bozukluk gözlenmeyen meme loblarına subklinik mastitis (SCM) tanısı amacıyla Kaliforniya Mastitis Testi (CMT) uygulandı. CMT’de sadece Ş ve +1 skorlar SCM pozitif olarak çalışmaya dahil edildi. Bu sonuçlar doğrultusunda 2 grup oluşturuldu; Grup I (n=40), CMT yönünden her 4 meme lobu da negatif olan ineklere ait serum ve süt örnekleri, Grup II (n=40)’ye ise CMT’ye göre pozitif olan serum ve süt örnekleri dahil edildi. Süt ve kan serumunda amiloid A ve CRP düzeyinin belirlenmesinde ticari ELISA kitleri kullanıldı. Çalışmada, ortalama AA düzeyleri sağlıklı olan serum ve süt örneklerinde sırasıyla 0,685±0,006 ng/ml; 0,665±0,007 ng/ml; SCM olanlarda ise sırasıyla 2,04±0,064 ng/ml ve 1,848±0,06 ng/ml belirlendi (p

Anahtar Kelimeler:

Amiloid A, Akut faz proteini, C- reaktif protein, İnek, Subklinik mastitis

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