Özgür GÖLGELİOĞLU, Mehmet ATEŞ, Güven GÜVENDİ, Sevim KANDİŞ, Servet KIZILDAĞ, Başar KOÇ, Ferda HOŞGÖRLER, Nazan UYSAL

Yaşlanmaya İlişkin Testiküler Bozulmalarda MMP-2 ve VEGF'nin Olası Etkileşimi

Amaç: Yaşlılarda, erektil disfonksiyon ve hipogonadizm, insan yaşamının uzaması nedeniyle daha önemli hale gelmiştir. Vasküler bozukluklar, kronik hipoksi, fibrozis ve hormonal değişiklikler yaşlı testisinde oluşan morfolojik değişikliklere yol açar. Vasküler endotelyal büyüme faktörü (VEGF) ve matriks metalloproteinaz-2 (MMP-2) hem morfolojik değişiklikler ile, hem de sperm hareketliliği ve canlılığı ile doğrudan ilişkilidir. VEGF ve MMP-2'nin yaşlı testisindeki yapısal ve fonksiyonel bozukluklara katkısı iyi bilinmemektedir. Bu çalışmanın amacı yaşlı testisinde VEGF ve MMP-2 düzeylerinin değişimini ve birbirleriyle etkileşimlerini belirlemektir. Yöntemler: Yaşlı sıçanlarda (26-30 ay) ve erişkin sıçanlarda (8-10 ay) testis dokusundaki MMP-2 ve VEGF düzeyleri ELISA yöntemi ile analiz edildi. Serum total testosteron düzeyleri immunoassay yöntemi kullanılarak analiz edildi. İstatistiksel analiz Mann Whitney-U testi kullanılarak yapıldı, p

Probable Interaction of MMP-2 and VEGF in Testicular Deteriorations Related to Aging

Objective: In the elderly, erectile dysfunction and hypogonadism have become more important due to the extension ofhuman life. The most important morphological changes of the aged testis are associated with vascular deterioration,chronic hypoxia, fibrosis and hormonal changes. Vascular Endothelial Growth Factor (VEGF) and MatrixMetalloproteinase-2 (MMP-2) are directly related to these changes along with sperm motility and viability. Thecontribution of VEGF and MMP-2 to the structural and functional disturbances in the aged testis is unknown. The aimof this study was to determine VEGF and MMP-2 levels in the elderly testis and their interactions with one another.Method: The testis tissues of aged rats (26-30 months) and adult rats (8-10 months) were analysed using the ELISAmethod to determine MMP-2 and VEGF levels. Serum total testosterone levels were analyzed using the immunoassaymethod. The statistical analysis was performed using the Mann Whitney-U test, p

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