Reserpinize Sıçanların Testis Dokusunda Oksidatif Stres Üzerine Thymoquinone'nin Etkisi
Amaç: Çalışma Reserpinize sıçanların testis dokusunda Thymoquinone (TQ)’ nun oksidan ve antioksidan etkinliğini araştırmak amacıyla yapıldı. Yöntemler: 18 adet Wistar Albino sıçan her grupta 6 sıçan olacak şekilde 3 eşit gruba ayrıldı. Kontrol(K) grubu: intraperitoneal %1 Tween 80 verildi. Reserpin(R): Reserpine verildi. Reserpine+TQ (R+T): Reserpine ve TQ verildi. Reserpin intraperitoneal 0.2 mg/kg enjekte edildi ve TQ intragastrik 10 mg/kg günde bir kere verildi. Deney ardışık 14 gün sürdü. Çalışmanın sonunda testis dokusunda toplam nitrik oksit (NOx), malondilaldehit (MDA) ve glutatyon (GSH) düzeyleri bakıldı. Bulgular: R grubunda testis dokusunun NOx düzeylerinde (p=0.001) ve MDA düzeylerinde istatistiksel olarak önemli bir artış oldu (p
Effects of Thymoquinone on Oxidative Stress in the Testicular Tissue of Reserpinized Rats
Objective: The objective of the study was to research the oxidant and antioxidant activity of Thymoquinone (TQ) inthe testicular tissue of Reserpinized rats.Methods: Eighteen rats were divided into three groups and each group had six animals: 1) Control (C) group: Receivedip. 1% Tween 80; 2) Reserpine (R) group: Received Reserpine; 3) Reserpine + TQ (R+T) group: Received Reserpineand TQ. Reserpine was injected intraperitoneally 0.2 mg/kg and TQ was administered intragastrically 10 mg/kg oncedaily. The rats were treated for 14 consecutive days. At the end of the study, total nitric oxide (NOx) levels,malondialdehyde (MDA) levels and glutathione (GSH) levels in the testicular tissue were examined.Results: A statistically significant increase was observed in the NOx levels (p=0.001) and MDA levels of testiculartissue in the R group (p
___
- 1. Erdemir F, Atilgan D, Firat F, et al. The effect of
Sertraline, Paroxetine, Fluoxetine and Escitalopram on
testicular tissue and oxidative stres parameters in rats.
Int Braz J Urol. 2014; 40: 100-8.
- 2. Abuja PM, Albertini R. Methods for monitoring
oxidative stress, lipid peroxidation and oxidation
resistance of lipoproteins. Clin Chim Acta. 2001; 306: 1-
17.
- 3. Squadrito GL, Pryor WA. Oxidative chemistry of nitric
oxide: the roles of superoxide, peroxynitrite, and
carbon dioxide. Free Radic. Biol. Med. 1998; 25: 392–
403.
- 4. Mukundan H, Bahadur AK, Kumar A, et al. Glutathine
level and its relation to radiation therapy in patients
with cancer of uterine cervix. Ind J Exp Biol. 1999; 37:
859–864.
- 5. Overington JP, Al-Lazikani B, Hopkins AL. How many
drug targets are there? Nat Rev Drug Discov. 2006; 5:
993-6.
- 6. Imming P, Sinning C, Meyer A. Drugs, their targets and
the nature and number of drug targets. Nat Rev Drug
Discov. 2006; 5: 821-34.
- 7. Sievert MK, Hajipour AR, Ruoho AE. Specific
derivatization of the vesicle monoamine transporter
with novel carrier-free radioiodinated reserpine and
tetrabenazine photoaffinity labels. Anal Biochem. 2007;
36: 68-78.
- 8. Mandela P, Chandley M, Xu YY, Zhu MY, Ordway GA.
Reserpine-induced reduction in norepinephrine
transporter function requires catecholamine storage
vesicles. Neurochem Int. 2010; 56: 760-7.
- 9. Rui Cui, Yunxiao Kang, Li Wang, et al. Testosterone
Propionate Exacerbates the Deficits of Nigrostriatal
Dopaminergic System and Downregulates Nrf2
Expression in Reserpine-Treated Aged Male Rats.
Front. Aging Neurosci. 2017; 9: 172.
- 10. Metzger RR, Brown JM, Sandoval V, et al. Inhibitory
effect of reserpine on dopamine transporter function.
Eur. J. Pharmacol. 2002; 456: 39-43.
- 11. Antkiewicz-Michaluk L, Wąsik A, Możdżeń E,
Romańska I, Michaluk J. Antidepressant-like effect of
tetrahydroisoquinoline amines in the animal model of
depressive disorder induced by repeated
administration of a low dose of reserpine: behavioral
and neurochemical studies in the rat. Neurotox Res.
2014; 26: 85-98.
- 12. Salih B, Sipahi T, Dönmez EO. Ancient nigella seeds
from Boyali Höyük in northcentral Turkey. J
Ethnopharmacol. 2009; 124: 416–20.
- 13. Ghosheh OA, Houdi AA, Crooks PA. High performance
liquid chromatographic analysis of the
pharmacologically active quinones and related
compounds in the oil of the black seed (Nigella sativa
L.). J Pharm Biomed Anal. 1999; 19: 757–62.
- 14. Kanter M, Coskun O, Uysal H. The antioxidative and
antihistaminic effect of Nigella sativa and its major
constituent, thymoquinone on ethanol-induced
gastricmucosal damage. Arch Toxicol. 2006; 80: 217–
24.
- 15. Hassanein KMA, El-Amir Y. Protective effects of
thymoquinone and avenanthramides on titanium
dioxide nanoparticles induced toxicity in SpragueDawley rats. Pathol Res Pract. 2017; 213: 13–22.
- 16. Awad AS, Kamel R, Sherief MA. Effect of
thymoquinone on hepatorenal dysfunction and
alteration of CYP3A1 and spermidine/ spermine N-1-
acetyl-transferase gene expression induced by renal
ischaemia-reperfusion in rats. J Pharm Pharmacol.
2011; 63: 1037–42.
- 17. Kanter M, Coskun O, Kalayci M, Buyukbas S, Cagavi F.
Neuroprotective effects of Nigella sativa on
experimental spinal cord injury in rats. Hum Exp
Toxicol. 2006; 25: 127–33.
- 18. Nili-Ahmadabadi A, Tavakoli F, Hasanzadeh GR,
Rahimi H, Sabzevari O. Protective effect of
pretreatment with thymoquinone against Aflatoxin B1
induced liver toxicity in mice. Daru. 2011; 19: 282–87.
19. Yaman I, Balikci E. Protective effects of Nigella sativa
against gentamicin-induced nephrotoxicity in rats. Exp.
Toxicol. Pathol. 2010; 62: 183–90.
- 20. Lupidi G, Scire A, Camaioni E et al. Thymoquinone, a
potential therapeutic agent of Nigella sativa, binds to
site I of human serum albumin. Phytomedicine. 2010;
17: 714–20.
- 21. Kustimur S, Kalkanci A, Akbulut G et al., The effect of
vaginal candidiasis on the levels of the oxidative
biomarkers in plasma and tissue samples of diabetic
rats. Mycopathologia. 2007; 164: 217-24.
- 22. Miranda KM, Espey MG, Wink DA. A rapid, simple
spectrophotometric method for simultaneous detection
of nitrate and nitrite. Nitric Oxide. 2001; 5: 62-71.
- 23. Rui Cui, Yunxiao Kang, Li Wang et al. Testosterone
Propionate Exacerbates the Deficits of Nigrostriatal
Dopaminergic System and Downregulates Nrf2
Expression in Reserpine-Treated Aged Male Rats.
Front. Aging Neurosci. 2017; 9: 172.
- 24. Abdel-Majeed S, Mohammad A, Shaima AB,
Mohammad R, Shaker A. Mousa. Inhibition property of
gren tea extract in relation to reserpine-induced
ribosomal strips of rough endoplasmic reticulum (rER)
of the rat kidney proximal tubule cells. The journal of
Toxicological Sciences. 2009; 34: 637-45.
- 25. Al-Bloushi S, Safer AM, Afzal M, Shaker A. Mousa.
Green tea modulates reserpine toxicity in animal
models. The journal of Toxicological Sciences. 2009; 34:
77-87.
- 26. Kıshımoto K, Fukuyado T, Sawamoto O, Kurısu K.
Influence of daıly subcutaneous administration of
reserpine for 4 weeks or 9 weeks before mating on
testıs, sperm and male fertılıty ın rats. The journal of
Toxicological Sciences. 1995; 20; 367-74.
- 27. Dıas PLR. Hıstometrıc analysıs of the effects of
reserpıne on the ınterstıtıal cells of the rat testıs. Br. J.
Exp. Path. 1982; 63: 518.
- 28. Sayeda MM, Hassaneinb KMA, Senosy W. Protective
effects of thymoquinone and l-cysteine on cadmiuminduced reproductive toxicity in rats. Toxicology
Reports. 2014; 1: 612–20.
- 29. Mabrouk A, Cheikh HB. Thymoquinone
supplementation reverses lead-induced oxidative stres
in adult rat testes. Gen. Physiol. Biophys. 2015; 34: 65–
72.
- 30. Hassanein KMA, El-Amir YO. Ameliorative effects of
thymoquinone on titanium dioxide nanoparticles
induced acute toxicity in rats International Journal of
Veterinary Science and Medicine. 2018; 6: 16–21.