Turgay SOLAK, Yavuz Sami SALİHOĞLU, Rabiye USLU ERDEMİR

Deneysel Testis Torsiyon-Detorsiyonunda Agmatine’nin Etkisinin Tc99m Perteknetat Sintigrafisi İle Değerlendirilmesi

Amaç: Bu çalışmanın amacı, sıçan modelinde tek taraflı testis torsiyon-detorsiyona bağlı oluşan testis hasarı üzerindeAgmatine uygulamasının koruyucu etkisini, Tc-99m perteknetat testis sintigrafi tetkikiyle göstermektir.Yöntemler: Otuz erkek Winstar Albino sıçanı, her biri on sıçan içeren üç gruba ayrıldı. Grup 1'de (kontrol) sahte bir işlemyapıldı. Grup 2’de sol testis torsiyonu ile 3 saat sonra detorsiyon yapıldı. Grup 3’te grup 2'deki ile aynı cerrahi işlemleruygulandı; detorsiyondan 15 dakika önce intraperitoneal Agmatine verildi. Tüm sıçanlarda Tc-99m perteknetat ile testissintigrafisi yapıldı. Tüm deney hayvanlarında histolojik inceleme ve malondialdehit (MDA) doku seviyelerinin ölçülmesiiçin orşiektomiler yapıldı.Bulgular: MDA düzeyi torsiyon-detorsiyon yapılan gruplarda (grup 1 ve 2) kontrol grubuna göre yüksek bulundu; grup3’te ise grup 2’ye göre azalmıştı. Histolojik hasar skoru Agmatine verilen grupta (grup 3) verilmeyen gruba (grup 2) göredüşük bulundu. İkinci grupta çoğunlukla 1 ve 2. derece testis yaralanması izlenmişken, grup 3’teki yaralanmalar 2-3derece arasında değişmekteydi. Testis sintigrafisindeki testislerin alım oranında bütün gruplar arasında anlamlı farkbulunmadı.Sonuç: Deneysel sıçan modelinde testiküler iskemi-reperfüzyon hasarını değerlendirmek için testis sintigrafisinin uygunbir tetkik olmadığı düşünüldü. Agmatine testis torsiyonundaki iskemi-reperfüzyon hasarını azaltmada etkin roloynayabilir.

Evaluation of the Effect of Agmatine with Tc-99m Pertechnetate Scintigraphy in Experimental Testicular Torsion-Detorsion

Objective: The aim of this study is to demonstrate the protective effect of Agmatine application on testicular damage caused by unilateral testicular torsion-detortion in the rat model by Tc-99m pertechnetate testis scintigraphy. Method: Thirty male Winstar Albino rats were divided into three groups, each containing ten rats. A sham operation was performed in group 1 (control). In group 2, torsion-detorsion procedures were performed on the left testicle. In group 3, the same surgical procedures were performed as in group 2; intraperitoneally Agmatine was given 15 minutes before detorsion. Testis scintigraphy was performed with Tc-99m pertechnetate in all rats. Orchiectomies were performed for histological examination and measurement of malondialdehyde (MDA) tissue levels in all experimental animals. Results: MDA levels were higher in torsion-detorsion groups (groups 1 and 2) than in the control group; in group 3, it decreased compared to group 2. Histological damage score was lower in the group given to Agmatine (group 3) compared to the group not given (group 2). In the second group, mostly 1 and 2 degree testicular injury signs were observed, however, the injuries in group 3 vary between 2-3 degrees. No significant difference was found between the groups in the uptake ratio of the testicle in testicular scintigraphy. Conclusion: In the experimental rat model, it was considered that testicular scintigraphy was not an appropriate test to evaluate the testicular ischemia-reperfusion injury. Agmatine may play an effective role in reducing ischemia-reperfusion injury in testicular torsion.

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