ERSEN ERASLAN, Ayhan TANYELİ, Mustafa CAN GÜLER, Elif POPALT

Ampelopsin, Deneysel Böbrek İskemi Reperfüzyon Hasarında Oksidatif Stresi ve İnflamatuvar Sitokin Seviyelerini Azaltmaktadır

Amaç: Böbrek iskemi/reperfüzyon (İ/R) hasarı, böbrek disfonksiyonuna neden olan ve önlem alınmazsa ölüme kadar gidebilen sıkıntılı bir süreçtir. Bu çalışma, böbrek İ/R hasarında farklı dozlarda kullanılan Ampelopsin’in (AMP) oksidatif stres ve proinflamatuvar sitokinler üzerine etkilerini araştırmayı amaçlamaktadır. Yöntemler: 32 adet Wistar Albino erkek sıçan rastgele 4 gruba ayrıldı (n=8): Sham, İ/R, İ/R+AMP 80mg/kg ve İ/R+AMP 160 mg/kg grupları. İ/R modelinde, renal pediküller 60 dakika süreyle klemplenerek iskemi oluşturuldu ve ardından klempler çıkarılarak 24 saat reperfüzyon uygulanarak İ/R modeli tamamlandı. AMP uygulaması ise reperfüzyon başlatılmadan hemen önce gerçekleştirildi. AMP’nin oksidan ve antioksidan moleküller ile proinflamatuvar sitokinler üzerine etkileri değerlendirildi. Bulgular: İ/R hasarı ile total antioksidan kapasite (TAK) ve süperoksit dismütaz (SOD) seviyeleri azalırken (p

Ampelopsin Reduces Oxidative Stress and Inflammatory Cytokine Levels in Experimental Kidney Ischemia Reperfusion Injury

Objective: Renal ischemia/reperfusion (I/R) injury is a distressed process leading to renal dysfunction and may result in death if precautions are not taken. The aim of this study was to investigate the effects of different Ampelopsin (AMP) doses on oxidative stress and proinflammatory cytokines in renal I/R injury. Methods: 32 Wistar Albino male rats were randomly divided into 4 groups (n=8): Sham, I/R, I/R+AMP 80 mg/kg and I/R+AMP 160 mg/kg groups. In the I/R model, renal pedicles were clamped for 60 minutes to create ischemia, and then the clamps were removed and reperfused for 24 hours to complete the I/R model. AMP administration was performed just before reperfusion was initiated. The effects of AMP on oxidant, antioxidant molecules and proinflammatory cytokines were evaluated. Results: The levels of total antioxidant status (TAS) and superoxide dismutase (SOD) decreased (p

PDF

___

1. Doyle JF, Forni LG. Acute kidney injury: shortterm and long-term effects. Crit Care. 2016; 20: 188.
2. Bellomo R, Kellum JA, Ronco C. Acute kidney injury. Lancet. 2012; 380: 756-66.
3. Manoeuvrier G, Bach-Ngohou K, Batard E, et al. Diagnostic performance of serum blood urea nitrogen to creatinine ratio for distinguishing prerenal from intrinsic acute kidney injury in the emergency department. BMC Nephrol. 2017; 18: 173.
4. Basile DP, Anderson MD, Sutton TA. Pathophysiology of acute kidney injury. Compr Physiol. 2012; 2: 1303-53.
5. Montagna G, Hofer CG, Torres AM. Impairment of cellular redox status and membrane protein activities in kidneys from rats with ischemic acute renal failure. Biochim Biophys Acta. 1998; 1407: 99-108.
6. Perez-Meseguer J, Torres-Gonzalez L, Gutierrez-Gonzalez JA, et al. Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model. BMC Complement Altern Med. 2019; 19: 186.
7. Zhou Y, Liang X, Chang H, et al. Ampelopsininduced autophagy protects breast cancer cells from apoptosis through Akt-mTOR pathway via endoplasmic reticulum stress. Cancer Sci. 2014; 105: 1279-87.
8. Wang Y, Ying L, Sun D, et al. Supercritical carbon dioxide extraction of bioactive compounds from Ampelopsis grossedentata stems: process optimization and antioxidant activity. Int J Mol Sci. 2011; 12: 6856-70.
9. Chang H, Peng X, Bai Q, et al. Ampelopsin suppresses breast carcinogenesis by inhibiting the mTOR signalling pathway. Carcinogenesis. 2014; 35: 1847-54.
10. Hou X, Zhang J, Ahmad H, et al. Evaluation of antioxidant activities of ampelopsin and its protective effect in lipopolysaccharide-induced oxidative stress piglets. PLoS One. 2014; 9: e108314.
11. Qi S, Xin Y, Guo Y, et al. Ampelopsin reduces endotoxic inflammation via repressing ROSmediated activation of PI3K/Akt/NF-kappaB signaling pathways. Int Immunopharmacol. 2012; 12: 278-87.
12. Ye XL, Lu LQ, Li W, et al. Oral administration of ampelopsin protects against acute brain injury in rats following focal cerebral ischemia. Exp Ther Med. 2017; 13: 1725-34.
13. Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Anal Biochem. 1979; 95: 351-8.
14. Bradley PP, Priebat DA, Christensen RD, et al. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982; 78: 206-9.
15. Sun Y, Oberley LW, Li Y. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem. 1988; 34: 497-500.
16. Harma M, Harma M, Kocyigit A, et al. Increased DNA damage in patients with complete hydatidiform mole. Mutat Res-Gen Tox En. 2005; 583: 49-54.
17. Friedewald JJ, Rabb H. Inflammatory cells in ischemic acute renal failure. Kidney Int. 2004; 66: 486-91.
18. .Eraslan E, Tanyeli A, Polat E, et al. 8-BrcADPR, a TRPM2 ion channel antagonist, inhibits renal ischemia-reperfusion injury. J Cell Physiol. 2019; 234: 4572-81.
19. Ye XL, Lu LQ, Li W, et al. Oral administration of ampelopsin protects against acute brain injury in rats following focal cerebral ischemia. Exp Ther Med. 2017; 13: 1725-34.
20. Qiu ZP, Zhou JX, Hu JJ, et al. Total flavonoids from Ampelopsis megalophylla suppress proliferation of vascular smooth muscle cells in vivo and in vitro. Braz J Pharm Sci. 2017; 53.
21. Levigne D, Tobalem M, Modarressi A, et al. Hyperglycemia Increases Susceptibility to Ischemic Necrosis. Biomed Research International. 2013.
22. Yoshitomi T, Hirayama A, Nagasaki Y. The ROS scavenging and renal protective effects of pH-responsive nitroxide radical-containing nanoparticles. Biomaterials. 2011; 32: 8021-8.
23. Hou X, Wang T, Ahmad H, et al. Ameliorative effect of ampelopsin on LPS-induced acute phase response in piglets. J Funct Foods. 2017; 35: 489-98.
24. Kou X, Li J, Liu X, et al. Ampelopsin attenuates the atrophy of skeletal muscle from d-gal-induced aging rats through activating AMPK/SIRT1/PGC-1alpha signaling cascade. Biomed Pharmacother. 2017; 90: 311-20.
25. Derya Güzel AT. p-Kumarik Asit, Renal İskemi Reperfüzyon Kaynaklı Akut Pulmoner Hasarı Azaltır. Sakarya Tıp Dergisi. 2018; 8: 644-9.
26. Bircan B, Cakir M, Kirbag S, et al. Effect of apelin hormone on renal ischemia/reperfusion induced oxidative damage in rats. Ren Fail. 2016; 38: 1122-8.
27. Song Q, Liu L, Yu J, et al. Dihydromyricetin attenuated Ang II induced cardiac fibroblasts proliferation related to inhibitory of oxidative stress. Eur J Pharmacol. 2017; 807: 159-67.
28. Lerman L, Textor SC. Pathophysiology of ischemic nephropathy. Urol Clin N Am. 2001; 28: 793-+.
29. Linas SL, Shanley PF, Whittenburg D, et al. Neutrophils accentuate ischemia-reperfusion injury in isolated perfused rat kidneys. Am J Physiol. 1988; 255(4 Pt 2): F728-35.
30. Wang YC, Liu QX, Zheng Q, et al. Dihydromyricetin Alleviates Sepsis-Induced Acute Lung Injury through Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis in Mice Model. Inflammation. 2019; 42: 1301-10.
31. Rabb H, Griffin MD, McKay DB, et al. Inflammation in AKI: Current Understanding, Key Questions, and Knowledge Gaps. Journal of the American Society of Nephrology. 2016; 27: 371-9.
32. Eraslan E, Tanyeli A, Polat E, et al. Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study. J Cell Biochem. 2019; 120: 17159-66.