Serhat KARACA, Şirin GÜNER ONUR, İlker TİNAY, Süheyla UYAR BOZKURT, Ali – Orcid Number: MENTEŞ

PRENATAL DÖNEMDEN İTİBAREN SİSTEMİK FLUOR VERİLEN SIÇANLARIN BÖBREK DOKULARINDA GÖRÜLEN APOPTOZİSİN İNCELENMESİ

Amaç: Bu çalışmanın amacı prenatal dönemden itibaren değişik konsantrasyonlarda sistemik fluora (F) maruz kalan sıçanlarda; F’nin, böbrek dokuları üzerindeki toksik etkilerinin incelenmesi ve meydana gelen histopatolojik değişikliklerin araştırılmasıdır. Gereç ve yöntem: Bu çalışmada, daha önceden prenatal dönemden itibaren içme suyu ile 0, 30 ve 100 ppm F dozları verilen ve 1., 3. ile 5. aylarda sakrifiye edilen sıçanlara ait böbrek dokuları kullanılmıştır. Histolojik değerlendirme için hematoksilen ve eozin (H&E), immünohistokimyasal inceleme için ise TUNEL yöntemi ile boyama yapılarak, tubulus ve glomerulus bölgelerinde meydana gelen apoptozis değerlendirilmiştir. Bulgular: 100 ppm F gruplarında tespit edilen apoptotik hücre sayısı, kontrol grubu ve 30 ppm F grubununa kıyasla anlamlı derecede yüksek bulunmuştur. Ayrıca hem tubulus hem de glomerulus bölgelerinde, tüm sıçanlarda böbrek dokularının 5. ay apoptozis değerlerinin, 1. ve 3. ay değerlerinden anlamlı şekilde olduğu tespit edilmiştir. Böbrek dokularında yapılan histolojik incelemelerde ise içme suyundaki F miktarının artması ile tübüler dilatasyon, glomeruluslarda atrofi ve nekroz alanları, vasküler konjesyon ve hemorajik kanama odakları gözlemlenmiştir. Sonuç: Yüksek F konsantrasyonunda sıçan böbrek dokularında apoptoziste artış ve belirgin bir yıkım gözlenmiştir. Anahtar sözcükler: fluor, böbrek, TUNEL, apoptozis Investigation of apoptosis in renal tissues of rats exposed from prenatal period to systemic fluoride ABSTRACT Aim: The aim of this study was to evaluate fluoride (F) induced toxic effects and histopathological changes on renal tissues of rats exposed to different concentrations of fluoride pre and postnatally. Material and Methods: Kidneys of the rats that had received 0, 30 and 100 ppm F in drinking water starting from the prenatal period and sacrificed at first, third and fifth months were used in this study. Renal tissues were sectioned and stained with hematoxylin and eosin (H&E) for histological analysis and with TUNEL technique for immunohistochemical evaluations of apoptosis in glomerular and tubular sections. Results: In the 100 ppm F group, the number of glomerular and tubular apoptotic cells were significantly higher than control and 30 ppm F groups. In all groups, higher apoptosis was detected in the 5th month than the 1st and 3rd months in glomerular and tubular sections and the differences were statistically significant. The transverse section of kidney of the 5th month rat group showed tubular dilatations, glomerular atrophies and necrosis areas, vascular congestions and hemorrhagic regions. Conclusion: High F doses administered in drinking water caused severe apoptosis and a marked destruction in rat kidney tissues. Key Words: fluoride, kidney, TUNEL, apoptosis

Anahtar Kelimeler:

fluor, böbrek, TUNEL

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