Anju BASNET, Surya Kant KALAUNI

Phytochemical Screening and Biological Activity Analysis of Some Selected Medicinal Plants of Ilam District of Nepal

Nepal is considered as a natural storehouse of medicinal plants with aromatic and medicinal values, being a land of diverse topography with the different weather conditions. More than 75% of the total population of Nepal relies on the traditional medicines for their primary healthcare and thus it is an important task to explore the chemical constituents and biological activities of plants grown in the local weather. So, we have selected ten medicinal plants as the experimental sample and extracted the contents in methanol by cold percolation method. The phytochemical screening revealed that glycosides, terpenoids, reducing sugars, polyphenols, and quinones were present in most of the plant extracts. In DPPH free radical scavenging test, the extracts of Nephrolepsis auriculata (46.22 μg/ml) and Rumex nepalensis (48.59 μg/ml) showed potent antioxidant activity. The highest total phenolic and total flavonoid contents are found in N. auriculata and R. nepalensis as 1076.73 & 964.56 mg/g GAE and 40.8 & 39.06 mg QE/g, respectively. The extract of Erythrina arborescens depicts significant antibacterial activity against the Gram-positive and Gram-negative bacteria. In brine shrimp lethality bioassay, the methanol extract of Zanthoxylum armatum (6.3 µg/mL) and Heracleum nepalense (88 µg/mL) showed considerable cytotoxicity as their LC50 values were found to be lower than 100 µg/mL. The results endorsed the notion behind the use of traditional medicinal plants to treat various diseases. So, these plant extracts could be used for isolating the bioactive compounds which could be used in the discovery process of noble drugs in the future.

Keywords:

Medicinal plants, antioxidants, phenolic flavonoids, antimicrobial,

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References; 1. Abdalla A.E.M., 2007. Egyptian mango by-product 2: Antioxidant and antimicrobial activities of extract and oil from mango seed kernel. Food Chemistry, 103(4), 1141–1152. DOI: 10.1016/j.foodchem.2006.10.026
2. Acharya, S. R., et al., 2014. Antioxidant and Antimicrobial Properties of Leaves of Lyonia ovalifolia Wallich. International Journal of Pharmaceutical & Biological Archives, 5(4), 76 -81.
3. Adhikary P., et.al, 2011. Phytochemical Screening and Anti-Microbial Properties of Medicinal Plants of Dhunkharka Community, Kavrepalanchowk, Nepal. International Journal of Pharmaceutical & Biological Archives, 2(6), 1663-1667.
4. Baskar N., Parimala D.B. and Mohan K. R., 2010. Anti-Cancer Activity of Methanol Extract of Root Bark of Erythrina variegata Linn. International Journal of Toxicological and Pharmacological Research, 2(2).
5. Biswas B. et.al, 2013. Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria. International Journal of Microbiology. DOI: 10.1155/2013/746165
6. Blois M. S., 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181:1199-1200.
7. Cavalieri S. J., Rankin I. D., et.al, 2005. Manual of Antimicrobial Susceptibility Testing. American Society for Microbiology, Washington, DC.
8. Ciulei I. 1982. Practical Manuals on the Industrial Utilization of Medicinal and Aromatic Plants: Methodology for the analysis of vegetable drugs. University of Bucarest, 1-10.
9. Devkota H.P., Adhikari B., Watanabe T., and Yahara S., 2018. Nonvolatile Chemical Constituents from the Leaves of Ligusticopsis wallichiana (DC.) Pimenov & Kljuykov and their Free Radical-Scavenging Activity. Journal of Analytical Methods in Chemistry, 1-8. DOI:10.1155/2018/1794650.
10. Dingel J., Reid W. W., Solomons G. L., 1953. The enzymic degradation of pectin and other polysaccharides. II-Application of the ‘Cup‐plate’ assay to the estimation of enzymes. Journal of the Science of Food and Agriculture, 4(3):149-155.
11. Dhote K., Dhote V., Khatri K., 2015. Phytochemical Screening and Pharmacological Activity in Punica granatum. Asian Journal of Pharmaceutical Education and Research, 4(4), 69-80.
12. Ghimire S.K., Sapkota I.B., Oli B.R., and Parajuli R., 2008. Non-Timber Forest Products of Nepal Himalaya; Database of Some Important Species Found in the Mountain Protected Areas and Surrounding Regions. WWF Nepal, Kathmandu, Nepal. NTFP of Nepal Himalaya.
13. Gonçalves S., Moreira E., Grosso C., Andrade P.B., Valentão P., Romano A., 2017. Phenolic profile, antioxidant activity, and enzyme inhibitory activities of extracts from aromatic plants used in Mediterranean diet. Journal of Food Science and Technology, 54(1):219-227. DOI: 10.1007/s13197-016-2453-z
14. Hamidi M. R., Jovanova B., Panovska T.K., 2014. Toxicоlogical evaluation of the plant products using Brine Shrimp (Artemia salina L.). Macedonian pharmaceutical bulletin, 60 (1) 9 – 18.
15. Jamuna, S., Paulsamy S., and Karthika K., 2012. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). Journal of Applied Pharmaceutical Science, 02 (07), 149-154.
16. Joshi R.K., 2016. Chemical constituents of leaf essential oils of Heracleum candicans Wall. ex DC from western Himalaya of Uttarakhand, India. American Journal of Essential Oils and Natural Products, 4(2), 01-04.
17. Kalita P., Tapan K.B., Pal T.K., Kalita R., 2013. Estimation of Total Flavonoids content and Anti-oxidant activities of Methanolic Whole plant extract of Biophytum Sensitivum Linn. Journal of Drug Delivery and Therapeutics, 3(4), 33-37. DOI: 10.22270/jddt.v3i4.546
18. Kasote D. M., Katyare S.S., Hegde M.V., and Bae H., 2015. Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. International Journal of Biological. Science, 11(8), 982-991. DOI: 10.7150/ijbs.12096
19. Kayat H.P., Gautam S.D., and Jha R.N., 2016. GC-MS analysis of hexane extract of Zanthoxylum armatum DC. Fruits. Journal of Pharmacognosy and Phytochemistry, 5(2): 58-62. 20. Kumar S., Joseph L., George M., and Bharti V., 2011. Antimicrobial Activity of Methanolic Extract of Rumex nepalensis Leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 3(4), 240-242.
21. Kunwar R. M., et.al, 2011. Medicinal and Aromatic Plants Network (MAPs-Net) Nepal: An open-access digital database. Banko Janakari, 21(1) DOI: 10.3126/banko.v21i1.9064
22. Kunwar et al., 2013. Medicinal plants, traditional medicine, markets, and management in far-west Nepal. Journal of Ethnobiology and Ethnomedicine, 9:24.
23. Manandhar S., Luitel S., and Dahal R.K., 2019. In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria. Journal of Tropical Medicine, 1-5. DOI: 10.1155/2019/1895340
24. Meena A. K., Bhavana P., Panda P., Sannd R., and Rao M.M., 2010. A review on Rubia cordifolia: Its phytoconstituents and therapeutic uses. Drug Invention Today, 2(5), 244-246.
25. Meyer B. N., et.al, 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, 45(5), 31-34. DOI: 10.1055/s-2007-971236
26. Nepal: General Info, Federation of Nepalese Chambers of Commerce and Industry (FNCCI).
27. Oliveira A.C. de et al., 2009. Total phenolic content and free radical scavenging activities of methanolic extract powders of tropical fruit residues. Food Chemistry, 115(2), 469–475. DOI: 10.1016/j.foodchem.2008.12.045
28. Parajuli R.R., 2013. Study on Local Uses of Medicinal Plants in Nayabazar, Pyang, and Jamuna VDCs of Ilam District. Nepal Journal of Science and Technology, 14(1), 57-66. DOI: 10.3126/njst.v14i1.8923
29. Parajuli, S. et.al, 2012. Antioxidant Activity, Total Phenol and Flavonoid Contents in Some Selected Medicinal Plants of Nepal. Journal of Health and Allied Sciences, 2(1), 27-31.
30. Rukmini K. and Suvarnalatha P. D., 2014. GC-MS Analysis and Phytochemical Screening of a rare Pteridophyte Nephrolepis Cardifolia (L.) Presl. from Tirumala Hills. International Journal of Pharma Research & Review, 3(12), 13-19.
31. Saxena M. et.al, 2012. Flavonoids and phenolic acids as antioxidants in plants and human health. International Journal of Pharmaceutical Sciences Review and Research, 16(2):130-134.
32. Saxena M., Saxena J., 2013. Phytochemistry of Medicinal Plants. Journal of pharmacognosy and Phytochemistry, 1(6), 168-182
33. Sen S. et al., 2010. Free Radicals, Antioxidants, Disease, and Phytomedicines: Current Status and Future Prospect. International Journal of Pharmaceutical Sciences Review and Research, 3(1), 91-100.
34. Shahidi F, Zhong Y., 2010. Novel antioxidants in food quality preservation and health promotion. European Journal of Lipid Science and Technology. 112(9), 930-940. DOI: 10.1002/ejlt.201000044
35. Ullah S., et.al, 2011. Antibacterial activity of some selected plants of Swat valley. Bioscience Research, 8(1), 15-18.
36. Waterhouse A. L., 2003. Determination of Total Phenolics. Current Protocols in Food Analytical Chemistry. John Wiley & Sons, New York. DOI: 10.1002/0471142913.fai0101s06
37. Yuan H., Ma Q., Li Y., and Piao G ., 2016. The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21(5): 559. DOI: 10.3390/molecules21050559
38. Zaidan M.R.S., et.al, 2005. In vitro screening of five local medicinal plants for antibacterial activity using the disc diffusion method. Tropical Biomedicine, 22(2), 165–170.