Kenan TUNÇ, Alican Bahadır SEMERCİ, Dilek İNCEÇAYIR, Mehmet Sağıroğlu SAĞIROĞLU

Antimicrobial Activity of Different Flower Extracts

In the present study, the antimicrobial activities of methanol extracts obtained from the flowers Muscari neglectum Punica granatum, Tussilago farfara, Taraxacum officinal, Wisteria sinensis, Syringa vulgaris, Cyclamen coum, Chaenomeles speciosa, Ornithogalum umbelatum, Allium siculum, Viburnum tinus and Eryngium bithynicum were evaluated. Extracts with methanol were prepared in soxhlet device. The evaluation process of the antimicrobial activities was performed using the disc diffusion method. The highest antimicrobial activity has been detected at the strain of S. aureus. The detected inhibition zone diameters of the flower extracts were evaluated to be 15.5 mm for V. tinus, 15 mm for Eryngium bithynicum and 14.5 mm for Punica granatum. It has also been found that the flower extracts of C. coum and M. neglectum only affect the C. albicans strain. The flowers of P. granatum has been found to be effective on all the test microorganisms. The extracts obtained from W. sinensis, T. farfara, T. officinal and O. umbelatum have no antimicrobial effect on any test microorganisms. It was found that 7 of the 12 plant species used in the study showed antimicrobial activity. In our study, it was observed that the extracts obtained from flowers were also effective in inhibiting microorganisms. To our knowledge, this is the first study to elucidate the antimicrobial acitivity of E. bithynicum, V. tinus and A. siculum’s flowers and our results how that the flowers of these species have the potential that can be used as antimicrobial natural sources.

Keywords:

. Flowers, Methanolic extract, Disc Difussion method,

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Ammar I. Ennouri m. Khemakhem B. Yangui T. Attia H. 2012. Variation in chemical composition and biological activities of two species of Opuntia flowers at four stages of flowering. Industrial Crops and Products 37 (2012) 34– 40
Arslan M. Erbil N. Murathan Z.T. 2018. Ardahan ve Çevresinde Yabani Olarak Yetişen Gilaburu Meyve Ekstraktının Antimikrobiyal, Antioksidan ve Antimutajenik Aktivitelerinin Araştırılması, GÜFBED/GUSTIJ (2018) 8 (1): 18-25
Bourgaud F. Gravot A. Milesi S. Gontier E. 2001. Production of plant secondary metabolites: a historical perspective. Plant science 161, 839-851.
Česoniene, L., Daubaras, R., Kraujalyte, V., Venskutonis, P.R. and Šarkinas, A., 2014. Antimicrobial activity of Viburnum opulus fruit juices and extracts, Journal fur Verbraucherschutz und Lebensmittelsicherheit, 9, 2, 129-132
Dahham S.S. Naiman-Ali M. Tabassum H. Khan M. 2010. Studies on Antibacterial and Antifungal Activity of Pomegranate (Punica granatum L.) American-Eurasian J. Agric. & Environ. Sci., 9 (3): 273-281, 2010
Dülger B. Gönüz A. 2004. Antimicrobial Activity of certain Plants used in Turkşsh Traditional medicine. Asian Journal of plant Sciences3(1): 104-107
Eroğlu-Özkan E. Demirci-Kayıran S. Toplan-Gülsoy G. Mataracı-Kara E. Kürkcüoğlu M. 2018. Identıfıcatıon Of Volatıle Compounds And Antımıcrobıal Actıvıtıes Of Muscarı neglectum Growıng In Turkey. Fresenius Environmental Bulletin 21:7 4674-4678
Ghaima-Kassim K. Hashim Makie N. Abdarlrasool-Ali S. 2013 Antibacterial and antioxidant activities of ethyl acetate extract of nettle (Urtica dioica) and dandelion (Taraxacum officinale). Journal of Applied Pharmaceutical Science Vol. 3 (05), pp. 096-099,
Hızlısoy, H., 2009. Çeşitli mikroorganizmalar üzerine gilaburunun antimikrobiyal etkisinin incelenmesi. Erciyes Üniversitesi, Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi
Hsu W.Y. Simonne A. Weşssman A. Kim J.M. 2010. Antimicrobial Activity of Greater Galangal [Alpinia galanga (Linn.) Swartz.] Flowers. Food Sci. Biotechnol. 19(4): 873-880 (2010)
Jeeva S. Johnson M. Aparna J.S. Irudayaraj V. 2011. Preliminary Phytochemical and Anti-Bacterial studies on Flowers of selected medicinal plants. Int. J. Med. Arom. Plants, Vol. 1, No. 2, pp. 107-114.
Lazarevic, J.S. Dordevic, A.S., Zlatkovic, B.K., Radulovic, S.N., Palic, R.M. 2010. Chemical composition and antioxidant and antimicrobial activities of essential oil of Allium sphaerocephalon L. subsp. sphaerocephalon (Liliaceae) inflorescences. J Sci Food Agric; 91: 322–329.ahboubi A. Asgarpanah J. Sadaghiyani P.N. Faizi M. Total phenolic and flavonoid content and antibacterial activity of Punica granatum L. var. pleniflora flowers (Golnar) against bacterial strains causing foodborne diseases. BMC Complementary and Alternative Medicine 15:366lcek J. Rop O. 2011. Fresh edible flowers of ornamental plants- A new source of nutraceutical foods. Trends in Food Science & Technology 22 (2011) 561-569.
Pavithra G.M. Saba S. Abhishktha S.N. Prashith K.T.R. Vinayaka K.S. 2013. Antioxidant and antimicrobial activity of flowers of Wendlandia thyrsoidea, Olea dioica, Lagerstroemia speciosa and Bombax malabaricum. Journal of Applied Pharmaceutical Science Vol. 3 (06), pp. 114-120
Rojas R. Bustamante b. Bauer J. Fernandez I. Alban J. Lock O. 2003. Antimicrobial activity of selected Peruvian medicinal plants. Journal of Ethnopharmacology 88, 199–204
Sajjadi S.T. Saboora A. Mohammadi P. 2016. Comparison of aglycon and glycosidic saponin extracts of Cyclamen coum tuber against Candida spp. Curr Med Mycol, 2016 Jun, 2(2): 40-44
Saratale G.R. Benelli G. Kumar G. Kim Su D.2018. Bio-fabrication of silver nanoparticles using the leaf extract of an ancient herbal medicine, dandelion (Taraxacum officinale),evaluation of their antioxidant, anticancer potential, and antimicrobial activity against phytopathogens. Environ Sci Pollut Res (2018) 25:10392–10406.
Sharifi-Rad J. Sureda A. Tenore G.C. Daglia M. Sharifi-Rad M. et al. 2017. Biological Activities of essental oils: From Plant chemoecology tı Traditional Healing Systems. Molecules, 22, 70;
Sharma A. Flores-Vallejo R.C. Cardoso-Taketa A. Villarreal M.L. 2017. Antibacterial activities of medicinal plants used in Mexican traditional medicine. Journal of Ethnopharmacology. 208(2017)264–329
Tunç K. Konca T. Hoş A. 2013. Punica granatum Linn. (nar) bitkisinin antibakteriyel etkisinin araştırılması. SAÜ. Fen Bil. Der. 17. Cilt, 2. Sayı, s. 167-172,
Türker A.U. Usta C. Biological screening of some Turkish medicinal plant extracts for antimicrobial and toxicity activities. Natural Product Research, Vol. 22, No. 2, 20 January 2008, 136–146
Usta C. Birinci-Yıldırım A. Uçar-Türker A. 2014. Antibacterial and antitumour activities of some plant grown in Turkey. Biotechnology & Biotechnological Equipment, Vol. 28, No. 2, 306-315,
Verpoorte R. Contin A. Memelink J. 2002. Biotechnology for the production of plant secondary metabolites. Phytochemistry Rewiews 1: 13-25.
Xianfei X. Xiaoqiang C. Shunying Z. Guolin Z. 2007. Chemical composition and antimicrobial activity of essential oils of Chaenomeles speciosa from China. Food Chemistry 100 (2007) 1312–1315