Kadir KINALIOGLU, Sinem AYDIN, Bahar BILGIN SOKMEN

Bazı Cladonia Likenlerinin Bitki Patojeni Bakterileri Üzerine Etkileri ve Bakır İndirgeme Antioksidan Kapasitesi Aktiviteleri

Bakteri ve mantar gibi mikroorganizmalar bitkilerde pek çok hastalığa yol açmaktadır. Patojenik mikroorganizmaların çoğu sıklıkla kullanılan antibiyotiklere ve sentetik fungisitlere direnç kazanmıştır. Likenler bir mantar, bir alg veya bir siyanobakteriyum arasındaki simbiyotik birliklerdir. Likenler ve metabolitlerinin sentetik kimyasallara karşı umut vaat edici alternatifler olabilecekleri gösterilmiştir. Bu çalışmanın amacı Cladonia stellaris, Cladonia pyxidata ve Cladonia furcata likenlerinin etil asetat ekstraktlarının antibakteriyal etkisini ortaya koymaktır. Ekstraktlar sokslet ekstraksiyonu ile elde edilmiştir. Antibakteriyal aktivite disk difüzyon metodu ile belirlenirken, antioksidan aktivite bakır indirgeme antioksidan kapasitesi (CUPRAC) metodu ile belirlenmiştir. En iyi antibakteriyal aktivite C. stellaris ekstraktında (25 mm) pozitif kontrol antibiyotiklerinden (ampisilin ve tetrasiklin) daha fazla olarak Pseudomonas syringae pv. syringae ve Erwinia carotovora subsp. carotovora'ya karşı bulunmuştur. CUPRAC aktivitesi C. furcata>C. pyxidata>C. stellaris şeklinde artmaktadır. Liken ekstraktlarından aktif bileşenlerin izolasyonu ve bu bileşiklerin biyolojik aktivitelerinin belirlenmesinde daha ileri çalışmalar gerekmektedir

Influence of Some Cladonia Lichens on Plant Pathogenic Bacteria and Copper Reducing Antioxidant Capacity Activities

Microorganisms such as bacteria and fungi lead many diseases in plants. A great deal of pathogenic microorganisms has gained resistance against frequently utilized antibiotics and synthetic fungicides. Lichens are symbiotic associations between a fungus, an algae or a cyanobacterium. Lichens and their metabolites are demonstrated to be encouraging alternatives to synthetic chemicals. The aim of this study was to reveal the antibacterial effect of ethyl acetate extracts of Cladonia stellaris, Cladonia pyxidata and Cladonia furcata lichen species. Extracts were obtained by Soxhlet extraction. Whereas antibacterial activity was determined by the disc diffusion method, antioxidant activity was determined by copper reducing antioxidant capacity (CUPRAC) method. The best antibacterial activity was found in C. stellaris extract (25 mm), which was stronger than the positive control antibiotics (ampicillin and tetracycline) against Pseudomonas syringae pv. syringae and Erwinia carotovora subsp. carotovora. The CUPRAC activity was increased in the following order: C. furcata>C. pyxidata>C. stellaris. Further studies are needed on isolation of active constituents from lichen extracts and their biological activity studies are to be worked out

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