Alper AKÇALI, Arif AKSU

Detection of norovirus infections in Canakkale withELISA and RT-PCR

Amaç: Norovirüsler dünya genelinde her yaş grubunda önemli enfeksiyonları ile ilgili ülke genelinde geniş bir veri bulunmamaktadır. norovirüslerle ilgili ilk araştırma olup, tanıda ELISA ve real time değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Klinik olarak ishali olan 92 vakadan dışkı örnekleri toplanmış olup çalışmaya kadar -20C'de saklanmıştır. Norovirüs antijeni RidaScreen ELISA kiti, GI ve GII moleküler saptaması RealStar Norovirüs RT-PCR kiti ile yapılmıştır. Bulgular: Çalışmada 16 (%17,4) örnek norovirüs için pozitif bulunmuştur. ELISA ile 92 örnekten 10 tanesi pozitiftir. PCR çalışmasında 4 örnekte internal kontrol çalışmamış olup, 88 örnekten 15 adedinde RT-PCR'de pozitiflik saptanmıştır. Bunlardan biri GI ve ondördü GII tespit edilmiştir. PCR'ı referans test olarak aldığımızda ELISA'nın duyarlılığı %60, özgüllüğü %98 bulunmuştur. Sonuç: Çanakkale gibi bir ilde bile norovirüs infeksiyonları birer infeksiyonlarının tanısı için altyapı oluşturulmalıdır. ELISA düşük bir duyarlılığa sahip olmakla birlikte RT-PCR'a göre ucuz olup, PCR inhibitörlerinden etkilenmemektedir

Norovirüs enfeksiyonlarının Çanakkale'de ELISA ve RT-PCR ile saptanmas

Purpose:Noroviruses are important agents of gastroenteritis worldwide in every age group. There is no large countrywide dataset available for norovirus infections in Turkey. This is the first investigation of noroviruses in Çanakkale, and we aimed to compare commercial ELISA and real time RT-PCR assays in clinical use. Material and Methods:Fecal samples from 92 clinical gastroenteritis cases were collected and stored at -20°C. NorovirusantigenwasinvestigatedusingRidaScreen ELISA and norovirus GI and GII molecular detection made with RealStar Norovirus RT-PCR kit 2.0. Results:We found that 16 (17.4%) samples were positive fornorovirusinourstudygroup.UsingELISA,10 samples were positive from 92 samples. In PCR analysis, internal control amplificationfailed for four samples. We found 15 of 88 samples positive for real time RT-PCR analysis; of these one was GI and 14 were GII. When we accepted PCR as the reference test, the sensitivity and specificity of ELISA were calculated as 60% and 98% respectively. Conclusion:Single cases of norovirus infection can be seen even in rural cities like Çanakkale. There is a need to set up diagnostic capabilities for norovirus infections. ELISA has a low sensitivity, but this method is cheaper than RT-PCR and is notaffected by PCR inhibitors.

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