Renal hücreli karsinomda bir heparanaz varyantı olan T5 oluşumunda miR-6814-5p'nin rolü

Amaç: Bu çalışmanın amacı insan renal hücre karsinomu (RHK) vakalarında insan heparanaz proteininin (HPSE) T5 adlı alternatif varyant proteininin aşırı ekspresyonunun nedeninin miR-6814-5p'nin artmış ekspresyonu olup olmadığını araştırmaktır. Ayrıca RHK vakalarının klinik parametreleri ile T5 ve miR-6814-5p ekspresyon seviyelerinin olası korelasyonu değerlendirilmiştir. Gereç ve Yöntem: T5 ve miR-6814-5p ekspresyon analizi, kullanıma hazır RHK cDNA panelinde qPCR yöntemi ile gerçekleştirildi. Bu panel, 10 evre-1, 5 evre-2, 13 evre-3 ve 11 evre-4 RHK hastalarının tümör dokularından ve 9 sağlıklı bireyden alınan normal böbrek dokularından elde edilen 48 cDNA örneğini içeriyordu. Bulgular: TNM evreleri, Fuhrman nükleer derece ve histolojik tip ile miR-6814-5p ve T5 ifadeleri arasında anlamlı bir ilişki bulunmadı. RHK tümör dokularında miR-6814-5p ekspresyon seviyesi, sağlıklı kontrollerden yaklaşık 8 kat daha yüksek ve T5 ekspresyon seviyesi yaklaşık 5 kat daha yüksekti. MiR-6814-5p ve T5 ekspresyon değişiklikleri, RHK vakalarının nötrofil / lenfosit oranı ile istatistiksel olarak anlamlı korelasyon gösterdi. Sonuç: MiR-6814-5p, RHK'de T5 oluşum mekanizmasında rol oynayabilir.

Anahtar Kelimeler:

Renal hücreli karsinom, heparanaz, gen ifade düzenlenmesi, miR-6814-5p

Role of miR-6814-5p for the formation of T5, a heparanase variant, in renal cell carcinoma

Purpose: The aim of this study is to investigate whether the overexpression of human heparanase protein (HPSE) alternative variant protein called T5 is caused by increased expression of miR-6814-5p in human renal cell carcinoma (RCC) cases. In addition, the possible correlation between the clinical parameters of RCC cases and the expression levels of T5 and miR-6814-5p was evaluated. Materials and Methods: T5 and miR-6814-5p expression analysis was performed on ready-to-use RCC cDNA panel by qPCR method. This panel included 48 cDNA samples obtained from tumor tissues of 10 stage-1, 5 stage-2, 13 stage-3 and 11 stage-4 RCC patients and normal kidney tissues from 9 healthy individuals. Results: There was no significant correlation between TNM stages, Fuhrman nuclear grade and histological type and miR-6814-5p and T5 expressions. The expression level of miR-6814-5p in RCC tumor tissues was about 8-fold higher and the T5 expression level about 5-fold higher than healthy controls. MiR-6814-5p and T5 expression changes were statistically significantly correlated with neutrophil/lymphocyte ratio of RCC cases. Conclusion: MiR-6814-5p may play a role in the formation mechanism of T5 in RCC.

Keywords:

Renal cell carcinoma, heparanase, gene expression regulation, miR-6814-5p,

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