Nila Kartika SARİ, Eriko PRAWESTİNİNGTYAS, Fatchiyah FATCHİYAH

Malang Doğu Java Endonezya Da Java Halkı ve Arap Aileler Arasında Genetik Varyasyon ve Allel Heterozigotluğunun Açığa Çıkarılması

Amaç: Bu çalışmanın amacı araplar ile evli Javalı ailelerdeki genetik çeşitliliğin ve babalık testi allel paternlerini belirlemektir. Materyal ve Metod: Üç ailenin üç kuşağından alınan beyaz kan hücrelerini kullandık : büyükanne-anne1 , baba-kız2, büyükbaba-anne, baba-kız3,dede, büyükbaba - anne, büyükanne - anne , baba-oğul . DNA kan örnekleri standart prosedür tuzla çöktürme ile izole edildi ve TPOX , D3S1358 , FGA , D5S818 , CSF1PO , D7S820 , D8S1179 , TH01 , VWA , D13S317 , D16S539 , D18S51 , D21S11 ve amelogenin primerleriyle 13 CODIS"e uygun olarak ile PCR ile çoğaltıldı. Parmak izi profili %8 poliakrilamid jel ile görüntülendi ve ChemiDoc jel Görüntüleme sistemi tarafından görüntüler alındı ve Quantity One yazılımı ile bant paterni yoğunluğu ölçüldü. Bulgular: Onüç CODIS markırı kullanarak elde edilen sonuçlarımız, babalık testinde genetik değişkenlik ve allelik heterozigot allellerinin ilk nesilden sonraki nesile arttığını gösterdi. Sonuç: Java-arap ailesinin genetik varyasyon ve allelik heterozigiteyi artırmasına sebeb olduğu sonucuna varılmıştır.

Anahtar Kelimeler:

Java-Arap etnik, DNA parmakizi; 13 CODIS

Revealing the Genetic Variation and Allele Heterozygote Javanese and Arab Families in Malang East Java Indonesia

Purpose: The purpose of this study is to identify genetic variability the family of Javanese married with Arab and their allele patterns for paternity testing. Material and Methods: We used human white blood cell from three generations of three families, consists of:1 grandmother-mother, father-daughter2, grandfather-mother, father-daughter3, grandfather, grandmother - mother, father-son. DNA blood samples were isolated by salting out standard procedure, and amplified by PCR with applying 13 CODIS which consists of TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11 and amelogenin primer. The Fingerprint profile was visualized by 8% polyacrylamide gel and took the picture by ChemiDoc gel Imaging and measure the intensity band pattern by Quantity One software Results: Our result showed that the genetic variability and heterozygote allele increasing by using the 13 CODIS markers from the first generation to the next generation with paternity testing from each family were matched. Conclusion: We can conclude that in a Javanese-Arab family ethnic seems stimulate the increasing genetic variation and allele heterozygote.

Keywords:

Javanese - Arab Ethnics DNA fingerprint, 13 CODIS,

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Yazışma Adresi / Address for Correspondence: Dr. Fatchiyah Fatchiyah Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University Malang, East Java, INDONESIA. e-mail: fatchiya@ub.ac.id, and fatchiya@gmail.com geliş tarihi/received :20.08.2012 kabul tarihi/accepted:24.09.2013