Hulya LEVENTERLER, Safiye TAGA, İbrahim Ferhat URUNSAK, İsmail Atilla ARİDOGAN, Suna SOLMAZ, Mehmet Turan CETİN, Nurten DİKMEN

İnsan Seminal Plazma ve Spermatozoa Homojenatlarında Malat Dehidrogenaz Aktivitesi

Amaç: Malat Dehidrogenaz, Krebs siklusunun önemli bir enzimi olup pek çok hücre metabolik aktiviteleri için bu enzime ihtiyaç duymaktadır. Normozoospermik, fertil ve infertil erkeklerin seminal plazma ve sperm homojenatlarında Malat Dehidrogenaz (NAD/NADP) aktivitesi ölçüldü. Ayrıca seminal plazma örneklerinde glukoz ve fruktoz konsantrasyonları tesbit edildi. Materyal ve Metod: Normozoospermik, fertil ve infertil erkeklerin seminal plazma ve sperm homojenatlarında Malat Dehidrogenaz (NAD/NADP) aktivitesi spektrofotometrik yöntemle ölçüldü. Semen analizi WHO kriterlerine göre değerlendirildi. Bulgular: Seminal plazmada MDH-NAD değeri (ortalama ± SD, mU/ml), astenoteratospermik (40.0±25.7) ve azospermik (38.0±43.6) gruplarda, normozoospermik, fertil erkeklerden (93.9±52.1) anlamlı düzeyde düşük bulunmuştur. Sperm homojenatlarında MDH-NAD değeri (ortalama ± SD, mU/ 20x106 sperm) teratospermik grupta (136.8±61.8) normozoospermik, fertil erkeklerden (87.3±26.5) anlamlı düzeyde yüksek bulunmuştur. Glukoz konsantrasyonu (mg/dl), astenoteratospermik (4.0±1.4) ve azospermik (15.4±6.4) gruplarda fertil erkeklerden anlamlı düzeyde (2.0±2.1) yüksek bulunmuştur. Benzer şekilde fruktoz konsantrasyonu (mg/dl), astenoteratospermik (706.6±143.3) ve azospermik (338.1±228.2) gruplarda fertil erkeklerden anlamlı düzeyde (184.7±124.8) yüksek bulunmuştur. Sonuç: Sperm, semendeki MDH aktivite kaynağının bir bölümü olabilir. Seminal plazmadaki MDH aktivitesi spermin enerji metabolizmasında önemli bir rol oynamaktadır. Krebs siklusunun ara substratları MDH"ın kontrolünde üretilmiş olabilir ve bu substratlar sperm motilitesi ve erkek infertilitesi için önemli olabilir.Anahtar Kelimeler:

Anahtar Kelimeler:

Erkek infertilitesi, malat dehidrogenaz, semen analizi, seminal plazma, spermatozoa homojenatı.

Malate dehydrogenase activity in human seminal plasma and spermatozoa homogenates

Purpose: Malate Dehydrogenase is an important enzyme of the Krebs cycle, most cells require this enzyme for their metabolic activity. We evaluated the Malate Dehydrogenase (NAD/NADP) activity in human seminal plasma and sperm homogenates in normozoospermic, fertile and infertile males. Also glucose and fructose concentrations were determined in the seminal plasma samples. Material and Methods: Malate Dehydrogenase (NAD/NADP) activity in human seminal plasma and sperm homogenates of normozoospermic and infertile males was determined by spectrophotometric method. Semen analysis was considered according to the WHO Criteria. Results: Malat Dehydrogenase-NAD value in seminal plasma (the mean ± SD, mU/ml) of asthenoteratospermic (40.0±25.7) and azospermic (38.0±43.6) groups were significantly lower than normozoospermic, (93.9±52.1) males. Malat Dehydrogenase-NAD value in sperm homogenates (the mean ± SD, mU/ 20x106 sperm) of teratospermic group (136.8±61.8) was significantly higher compared to the normozoospermic (87.3±26.5) males. Glucose concentration (mg/dl) in asthenoteratospermic (4.0±1.4) and azospermic (15.4±6.4) groups were significantly higher than fertile (2.0±2.1) males. Also fructose concentration (mg/dl) in asthenoteratospermic (706.6±143.3) and azospermic (338.1±228.2) groups were significantly high compared to the normozoospermic (184.7±124.8) group. Conclusion: Sperm may be some part of the source of Malat Dehydrogenase activity in semen. Malat Dehydrogenase activity in seminal plasma has an important role on energy metabolism of sperm. Intermediate substrates of Krebs cycle might have been produced under the control of Malat Dehydrogenase and these substrates may be important for sperm motility and male infertility.Key words:

Keywords:

Malate dehydrogenase, male infertility, semen analysis, seminal plasma, spermatozoa homogenates.,

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Yazışma Adresi / Address for Correspondence: Hülya Leventerler Faculty of Medicine, Cukurova University, Gynecology and Obstetric Department Center of Assisted Reproduction Adana, Turkey Phone: 03223386060-3831
E-mail: hleventerler@cu.edu.tr geliş tarihi/received :24.09.2013 kabul tarihi/accepted:07.12.2013