Melek TUNÇ ATA, Ayşegül ÇÖRT DÖNMEZ, Barış Özgür DÖNMEZ, İsmail Hakkı AKBUDAK, Vural KÜÇÜKATAY

İleal interpozisyon, metabolik sendromlu sıçanlarda oksidan-antioksidan enzimler yoluyla oksidatif stresi azaltır

Amaç: Bu çalışmanın amacı ileal transpozisyonun (IT) hem metabolik sendrom (MetS) sıçanları hem de sağlıklı kontrollerdeki toplam antioksidan durum (TAS), toplam oksidan durum (TOS), Oksidatif Stres İndeksi (OSI), Süperoksit dismutaz (SOD), Nikotinamid adenin dinükleotid fosfat oksidaz (NOX), Katalaz (CAT), İndirgenmiş Glutatyon (GSH)’un plazma seviyeleri üzerindeki etkisini incelemektir. Gereç ve Yöntem: MetS modeline göre, yeni doğan erkek Wistar albino sıçanlara 0, 2, 4, 6, 8 ve 10. günlerde monosodyum glutamat (MSG) (4 mg/g) verildi. Kontrol grubuna sadece salin enjekte edildi. 5. ayda sham ve IT hayvanlara seçilmiş cerrahi uygulandı. Ameliyattan 2 ay sonra plazmada TOS, TAS, OSI, SOD, NOX, CAT, GSH seviyeleri değerlendirildi. Bulgular: IT prosedürü MetS + IT grubunda MetS grubuna kıyasla SOD ve CAT düzeylerini önemli ölçüde artırdı (SOD; MetS 1.75 ± 0.04, MetS+IT 2.1 ± 0.15, CAT; MetS 32.02 ± 1.73, MetS+IT 41.64 ± 1.18). Beklendiği gibi, MetS+IT sıçanlarda MetS sıçanlara göre GSH seviyeleri artarken, aradaki fark anlamlı değildi (MetS 243.31 ± 6.36, MetS+IT 269.76 ± 9.17). NOX aktivitesi MetS+IT grubunda MetS ve MetS+S gruplarına göre anlamlı derecede düşüktü (MetS 610.35 ± 26.25, MetS+IT 348.86 ± 14.12). Sonuç: Bu veriler, IT cerrahisinin MetS ile ilişkili oksidatif strese karşı iyileştirici etkisini ortaya koydu. Mevcut veriler, sistemik oksidan ve antioksidan yanıtları modüle ederek MetS'li sıçanlarda oksidatif hasarı azaltmak için IT cerrahisini etkili bir strateji olarak desteklemektedir.

Ileal interposition reduces oxidative stress via oxidant-antioxidant enzymes in rats with metabolic syndrome

Purpose: This study aims to examine the effect of ileal transposition (IT) on plasma levels of the total antioxidant status (TAS), total oxidant status (TOS), Oxidative Stress Index (OSI), Superoxide dismutase (SOD), Nicotinamide adenine dinucleotide phosphate oxidase (NOX), Catalase (CAT), Reduced Glutathione (GSH) in both rats with Metabolic syndrome (MetS)and healthy controls. Materials and Methods: In the MetS model, newborn male Wistar albino rats were given MSG (4 g/mg) on days 0, 2, 4, 6, 8, and 10. The control group was injected only saline. In the 5th month, sham and IT animals underwent selected surgery. 2 months after surgery TOS, TAS, OSI, SOD, NOX, CAT, and GSH levels were assessed in the plasma. Results: IT procedure significantly increased SOD and CAT levels in MetS + IT group when compared to the MetS group (SOD; MetS 1.75 ± 0.04, MetS+IT 2.1 ± 0.15, CAT; MetS 32.02 ± 1.73, MetS+IT 41.64 ± 1.18,). As expected, while GSH levels was increased in MetS+IT rats compared to MetS rats, but the difference was not significant (MetS 243.31 ± 6.36, MetS+IT 269.76 ± 9.17). The NOX activity was significantly lower in MetS+IT group than MetS and MetS+S groups (MetS 610.35 ± 26.25, MetS+IT 348.86 ± 14.12). Conclusion: These data revealed the healing effect of IT surgery against oxidative stress associated with MetS. The available data endorses IT surgery as an effective strategy to reduce oxidative damage in rats with MetS by modulating systemic oxidant and antioxidant responses.

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