Alperen SARITAŞ, Gülan ALBAŞ KURT, Abdülkadir BİLİR, Ozan TURAMANLAR, Fatih GÜRSOY

Erken dönem civciv embriyo modelinde dimenhidrinatın nöral tüp gelişimi üzerine etkisi

Amaç: Bu çalışmada dimenhidrinat’ın erken dönem civciv embriyo modelinde nöral tüp gelişimi üzerindeki etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışmada 32 fertil, spesifik patojen olmayan yumurta kullanıldı. Tüm yumurtalar her bir grupta 8 adet olacak şekilde dört gruba ayrıldı. İnkübasyonun 28. saatinde deney gruplarına üç farklı dozda (0,7, 1,4, 2,1 mg/kg) dimenhidrinat ve kontrol grubuna serum fizyolojik enjekte edildi. İnkübasyonun 48. saatinde tüm embriyolar diseke edildi ve ışık mikroskobu altında histolojik olarak değerlendirildi. Bulgular: Kontrol, düşük, orta ve yüksek doz grubu embriyoları morfolojik olarak değerlendirildiğinde, kraniokaudal uzunluk ortalamaları sırasıyla; 8493,73±1342,90 µm, 5743,61±770,3 µm, 5572,62±315,32 µm, 5306,55±1135,99 µm, somit sayıları ortalamaları sırasıyla; 15,91±1,62, 15,38±1,19, 15,13±0,83, 10,63±5,15‘dir. Dimenhidrinat dozundaki artışa bağlı olarak kraniokaudal uzunluklarının ve somit sayılarının azaldığı tespit edildi. Dimenhidrinat doz artışına bağlı olarak nöral tüp açıklığının arttığı belirlendi . Sonuç: Bu çalışmada, erken dönem civciv embriyo modelinde dimenhidrinat uygulanan gruplarda doz artışına bağlı olarak nörogenezisin ve morfolojik gelişimin olumsuz etkilendiği görüldü.

Anahtar Kelimeler:

Civciv embriyosu, dimenhidrinat, nöral tüp defekti, morfoloji

Effect of dimenhydrinate on neural tube development in early chick embryo model

Purpose: The aim of this study was to investigate the effects of dimenhydrinate on neural tube development in an early chick embryo model. Materials and Methods: 32 fertile, specific pathogen free eggs were used in the study. All eggs were divided into four groups with 8 eggs in each group. At the 28th hour of the incubation, three different doses of dimenhydrinate (0.7, 1.4, 2.1 mg/kg) were injected into the experimental groups and physiological saline was injected into the control group. At 48th hour of incubation, all embryos were dissected and evaluated histologically under the light microscope. Results: When the control, low, medium and high dose group embryos were evaluated morphologically, their mean craniocaudal lengths were 8493.73±1342.90 µm, 5743.61±770.3 µm, 5572.62±315.32 µm, 5306.55±1135.99 µm respectively, mean of somite numbers were 15.91±1.62, 15.38±1.19, 15.13±0.83, 10.63±5.15 respectively. Craniocaudal lengths and somite numbers decreased depending on the increase in the dimenhydrinate dose. The incidence of open neural tube increased due to the increase in dimenhydrinate dose. Conclusion: Neurogenesis and morphological development were negatively affected due to the increase in dose in the groups treated with dimenhydrinate in the early chick embryo model.

Keywords:

Chick embryo, dimenhydrinate, neural tube defect, morphology,

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