Memet RENCBER, Abdullah OĞUZ, Eda YİLDİZHAN

Ratlarda Rosmarinik Asit’in Akut Pankreatit Üzerindeki Koruyucu Etkinliğinin İncelenmesi

Amaç: Akut pankreatit her yüz bin kişide 5-35 kişi arasında görülen ciddi bir hastane yatış sebebidir. Hastane yatışlarının yaklaşık yüzde 5-10’u ölümle sonuçlanmaktadır. Pankreatit durumunun tedavisi konusunda her geçen gün yeni çalışmalar planlanmaktır. Rozmarinik asit’in yapılan birçok çalışmada antioksidan özelliğe sahip olduğu gösterilmiştir. Bizde yaptığımız bu çalışmada Rosmarinik asit’in akut pankreatit üzerinde koruyucu etkinliğini incelemeyi amaçladık. Materyal/ Metod: : Deney süresince toplam 28 hayvan kullanılmış olup, her grupta 7 hayvan olacak şekilde 4 grup oluşturuldu. Grup 1 kontrol grubu olup deney süresince hiçbir ilaç kullanılmadı. Grup 2’deki ratlara saatte bir 75 μg/kg Cerulein birer saat arayla intraperitoneal olarak, toplam dört defa enjekte edildi. Grup 3 deney hayvanlarına peroral gavaj yoluyla 50 mg/kg dozda Rosmarinik asit verildi. Grup 4’deki ratlara ise saatte bir 75 μg/kg Cerulein intraperitoneal olarak toplam dört defa olmak üzere enjekte edildikten sonra, Rosmarinik asit peroral gavaj yoluyla 50 mg/kg verildi. Sonrasında çalışma gruplarındaki tüm hayvanlar ekzanguinasyon ile sakrifiye edildi, kalpten alınan kan örnekleri ve pankreas dokusu inceleme amacıyla alındı. Bulgular: Pankreas dokularının ışık mikroskobik incelemelerinde akut pankreatit grubunda dokularda nekroz, ödem ve inflamasyon görüldü. Rosmarinik asit grubunun hem histopatolojik hem de serum değerlerinin kontrol grubuna yakın olduğu görüldü. Akut pankreatit sonrası Rosmarinik asit kullanımının pakreas dokularda ve kan değerlerinde pozitif etkili olduğu, fakat yine de tamamen iyileşmeye neden olmadığı tespit edildi. Sonuç: Akut pankreatit durumunda Rosmarinik asitin kısmen iyileştirici özelliğinin olduğu fakat yine de tam bir iyileşme sağlamadığı kanaatine varıldı.

Anahtar Kelimeler:

Akut pankreatit, Cerulein, Rosmarinik Asit

Protective Efficacy of Rosmarinic Acid on Acute Pancreatitis in Rats

Abstract Aim: Acute pancreatitis is a serious disease, with an incidence of 5 - 35 in 100,000 individual. New studies are constantly planned for the treatment of pancreatitis. Many studies have shown that Rosmarinic acid has antioxidant properties. In this study, we examined the protective effect of Rosmarinic acid on acute pancreatitis. Material and Methods: A total of 28 animals were used during the experiment, and 4 groups were formed with 7 animals in each group. Group 1 is the control group. The rats in Group 2 were administered 75 μg/kg Cerulein every hour intraperitoneally at one hour intervals, a total of four times. Group 3 experimental animals were given 50 mg/kg Rosmarinic acid by per oral gavage. The rats in group 4 were given 50 mg/kg Rosmarinic acid per oral gavage after 75 μg/kg Cerulein was injected intraperitoneally every hour for a total of four times. Afterwards, all animals were sacrificed by exsanguination, blood samples and pancreatic tissue were taken for examination. Results: Examination of pancreatic tissues revealed necrosis, edema and inflammation in the acute pancreatitis group. Both histopathological and serum values of the rosmarinic acid group were close to the control group. The use of Rosmarinic acid after acute pancreatitis had a positive effect on the pacreatic tissues and blood values, but still did not cause complete recovery. Conclusion: In the case of acute pancreatitis, it was concluded that rosmarinic acid has a partial curative effect, but still does not provide a full recovery.

Keywords:

Acute pancreatitis, Cerulein, Rosmarinic Acid,

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