Duygu Fevziye VURAL, Hatice MUTLU EYİSON, Dilşad ÖZERKAN, Suna CEBESOY

The effect of melatonin on rat soleus muscle treated with carbon tetrachloride

Antioxidants are known to restrains various tissue damage caused by the administration of carbon tetrachloride (CCl4). This study examined whether melatonin (MEL), a molecule known to have antioxidant properties, has a protective effect on the rat soleus muscle, where toxic damage is caused by the application of CCl4. In the study, eighteen albino-type male Wistar rats were used and divided into three groups as Control Group (group 1), CCl4 group (group 2) and CCl4 + MEL group (group 3). End of the 12 weeks, blood samples were taken as intracardiac from the rats under ketamine/rompun anesthesia, and the soleus muscles of the rats were removed. Tissue samples were subjected to routine preparation procedures for light microscopy. Sections 5 µm thick taken and stained with hematoxylin-eosin (HE) for histopathological examinations and Masson’s Trichrome stain for fibrosis formations. In conclusion, the CCl4 group displayed muscular hypertrophy, fiber orientation dysfunction and atrophy in some areas. In addition, fibrosis was spotted around the venous and nerve plexuses. In contrast to the CCl4 group, the melatonin group displayed no fibrosis and maintained tissue integrity. Therefore, when comparing CCl4+MEL and CCl4 groups, it was observed that melatonin had a stabilizing or even curative effect on the injuries.

Keywords:

Skeletal muscle, soleus muscle, melatonin, CCl4,

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Lilić, L.M., Toskić, D., Stefanović, R.Ž., Mekić, B.B., Ilić, I.R., Stojanović, N.M., The role of mast cells in carbon tetrachloride induced rat skeletal muscle tissue damage, Acta Medica Medianae, 58(2) (2019), 11-15. https://doi.org/10.5633/amm.2019.0202
Adaramoye, O.A., Comparative effects of vitamin E and kolaviron (a biflavonoid from Garcinia kola) on carbon tetrachloride-induced renal oxidative damage in mice, Pakistan Journal of Biological Science, 12 (2009), 1146-1151. https://doi.org/10.3923/pjbs.2009.1146.1151
Ahmad, F.F., Cowan, D.L., Sun, A.Y., Detection of free radical formation in various tissues after acute carbon tetrachloride administration in gerbil, Life sciences, 41 (1987), 2469-28475. http://doi.org/10.1016/0024-3205(87)90673-4
Ozturk, F., Ucar, M., Ozturk, I.C., Vardi. N., Batcioglu, K., Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats, Urology, 62 (2003), 353-356. https://doi.org/10.1016/S0090-4295(03)00255-3
Pope, A.M., Rall D.P., Environmental Medicine: İntegrating a Missing Element in to Medical Education, Case Study 8: Carbon Tetrachloride, National Academy Press, Washington, D.C., 1995.
Rechnagel, R.O., Glende, E.A., Plaa, G.L., Carbon tetrachloride hepatotoxicity: an example of lethal cleavage, CRC critical reviews in toxicology, 2 (1973), 263-297. https://doi.org/10.3109/10408447309082019
Rikans, L.E., Hornbrook, K.R., Cai, Y., Carbon tetrachloride hepatotoxicity as a function of age in female Fischer 344 rats, Mechanisms of ageing and development, 76 (1994), 89-99. https://doi.org/10.1016/0047-6374(94)91584-9
Grebe, N.M., Sarafin, R.E., Strenth, C.R., Zilioli, S., Pair-bonding, fatherhood, and the role of testosterone: A meta-analytic review, Neuroscience Biobehavioral Reviews, 98 (2019), 221-233. https://doi.org/10.1016/j.neubiorev.2019.01.010
Shenoy, K.A., Somayaji, S.N., Bairy, K.L., Hepatoprotective effects of Ginkgo biloba against carbon tetrachloride induced hepatic injury in rats, Indian Journal of Pharmacology, 33 (2001), 260-266.
Yuan, L.P., Chen, F.H., Ling, L., Bo, H., Chen, Z.W., Li, F., Zhong, M.M., Xia, L.J., Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats, Jounal of Pharmacy and Pharmacology, 60(10) (2008), 1393-1402. https://doi.org/10.1016/j.jep.2008.01.1010
Upur, H., Amat, N., Blazekovi, B., Talip, A., Protective effect of Cichorium glandulosum root extract on carbon tetrachloride-induced and galactosamine-induced hepatotoxicity in mice, Food and Chemical Toxicology Food Chem Toxicol, 47(8) (2009), 2022-2030. https://doi.org/10.1016/j.fct.2009.05.022
Adewole, S., Salako, A., Doherty, O., Naicker, T., Effect of melatonin on carbon tetrachloride-induced kidney injury in Wistar rats, African Journal of Biomedical Research, 10(2) (2010), 153-164. https://doi.org/10.4314/ajbr.v10i2.50619
Reiter, R.J., Neuroendocrine effects of light, International Journal of Biometeorology, 35 (1991), 169-175.
Reiter, R.J., Melchiorri, D., Sewerynek, E., Poeggeler, B., Barlow-Walden, L., Chuang, J., Ortiz, G.G., Acuña-Castroviejo, D., A review of the evidence supporting melatonin’s role as an antioxidant, J. Pineal Res. 19 (1995), 149-165. https://doi.org/10.1111/j.1600-079X.1995.tb00133.x
Brzezinski, A., Melatonin in humans, The New England Journal of Medicine, 336 (1997), 186-195. https://doi.org/10.1056/NEJM19970116360306
Arushanian, E.B., Schetinin, E.V., Melatonin as a universal modulator of any pathological processes, Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia, 60(1) (2016), 79-88.
Carpentieri, A., De Barboza, G.D., Areco, V., López, M.P., De Talamoni, N. T., New perspectives in melatonin uses, Pharmacological research, 65(4) (2012), 437-444. https://doi.org/10.1016/j.phrs.2012.01.003
Navarro-Alarcón, M., Ruiz-Ojeda, F.J., Blanca-Herrera, R.M., A-Serrano, M. M., Acuña-Castroviejo, D., Fernández-Vázquez, G., Agil, A., Melatonin and metabolic regulation: a review, Food & function, 5(11) (2014), 2806-2832. https://doi.org/10.1039/C4FO00317A
Coto-Montes, A., Boga, J.A., Tan, D.X., Reiter, R.J., Melatonin as a potential agent in the treatment of sarcopenia, International journal of molecular sciences, 17(10) (2016), 1771. https://doi.org/10.3390/ijms17101771
Maarman, G.J., Reiter, R.J., Melatonin therapy for blunt trauma and strenuous exercise: A mechanism involving cytokines, NFκB, Akt, MAFBX and MURF-1, Journal of Sports Sciences, 36(16) (2018), 1897-1901. https://doi.org/10.1080/02640414.2018.1424491
Majidinia, M., Reiter, R.J., Shakouri, S.K., Mohebbi, I., Rastegar, M., Kaviani, M., Yousefi, B., The multiple functions of melatonin in regenerative medicine, Ageing research reviews, 45 (2018), 33-52. https://doi.org/10.1016/j.arr.2018.04.003
Zavodnik, L.B., Zavodnik, I.B., Lapshina, E.A., Belonovskaya, E.B., Martinchik, D.I., Kravchuk, R.I., Bryszewska, M., Reiter. R.J., Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats, Cell Biochemistry and Function, 23 (2005), 353-359. https://doi.org/10.1002/cbf.1160
Kuş, İ. & Sarsılmaz, M., Pineal bezin morfolojik yapısı ve fonksiyonları, Türkiye Klinikleri Tıp Bilimleri Dergisi, 22 (2002), 221-226.
Guerrero, J.M., Reiter, R.J., A brief survey of pineal gland-immune system interrelationships, Endocrine Research, 18 (1992), 91-113. https://doi.org/10.1080/07435809209035401
Chen, B., You, W., Shan, T., The regulatory role of melatonin in skeletal muscle, Journal of Muscle Research and Cell Motility, 41(2) (2020), 191-198. https://doi.org/10.1007/s10974-020-09578-3
Lepper, C., Partridge, T.A., Fan, C.M., An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration, Development, 138(17) (2011), 3639-3646. https://doi.org/10.1242/dev.067595
Murphy, M.M., Lawson, J.A., Mathew, S.J., Hutcheson, D.A., Kardon, G., Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration, Development, 138(17) (2011), 3625-3637. https://doi.org/10.1242/dev.064162
Sambasivan, R., Yao, R., Kissenpfennig, A., Van Wittenberghe, L. Paldi, A., Gayraud-Morel, B., Galy, A., Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration, Development, 138(17) (2011), 3647-3656. https://doi.org/10.1242/dev.067587
Suwa, M., Miyazaki, T., Nakamura, T., Sasaki, S., Ohmori, H., Katsuta, S., Hereditary dominance of fast-twitch fibers in skeletal muscles and relation of thyroid hormone under physiological conditions in rats, Acta Anatomica (Basel), 162 (1998), 40-45. https://doi.org/10.1159/000046467
http://www.ask.com/question/where-is-the-soleus-muscle-located.
Stratos, I., Richter, N., Rotter, R., Li, Z., Zechner, D., Mittlmeier, T., Vollmar, B., Melatonin restores muscle regeneration and enhances muscle function after crush injury in rats, Journal of pineal research, 52(1) (2012), 62-70. https://doi.org/10.1111/j.1600-079X.2011.00919.x
Popović, D., Đukić, D., Katić, V., Jović, Z., Jović, M., Lalić, J., Sokolović, D., Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride, Life sciences, 157 (2016), 168-177. https://doi.org/10.1016/j.lfs.2016.06.007
Radulović, N.S., Randjelović, P.J., Stojanović, N.M., Ilić, I.R., Miltojević, A.B., Influence of methyl and isopropyl n-methyl antranilates on carbon tetrachloride-induced changes in rat liver morphology and function, Facta universitatis-series, Physics, Chemistry and Technology, 11(1) (2013), 67-73. https://doi.org/10.2298/FUPCT1301067R
Abraham, P., Wilfred, G., Cathrine, S.P., Oxidative damage to the lipids and proteins pf the lungs, testis and kidney of rats during carbon tetrachloride intoxication, Clinica Chimica Acta., 289 (1999), 177-179. https://doi.org/10.1016/s0009-8981(99)00140-0
Tirkey, N., Pilkhwal, S., Kuhad, A., Chopra, K., Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbontetrachloride in rat liver and kidney, BMC Pharmacoogyl, (5)2 (2005), 1-8. https://doi.org/10.1186/1471-2210-5-2
Kus, I., Ogeturk, M., Oner, H., Sahin, S., Yekeler, H., Mustafa, S., Protective effects of melatonin against carbon tetrachloride-induced hepatotoxicity in rats: a light microscopic and biochemical study, Cell Biochemistry and Function, 23 (2005), 169-174. https://doi.org/10.1002/cbf.1136
Erdem, M., Bostan, B., Güneş, T., Özkan, F., Sen, C., Özyurt, H., Köseoğlu, R. D., Erdoğan, H., Protective effects of melatonin on ischemia-reperfusion injury of skeletal muscle, Eklem Hastalık Cerrahisi, 21(3) (2010), 166-71.
Sokolović, D.T., Lilić, L., Milenković, V., Stefanović, R., Ilić, T.P., Mekić, B., Ilić, I.R., Effects of melatonin on oxidative stress parameters and pathohistological changes in rat skeletal muscle tissue following carbon tetrachloride application, Saudi Pharmaceutical Journal, 26(7) (2018), 1044-1050. https://doi.org/10.1016/j.jsps.2018.05.013