ALPHA-FETOPROTEIN GENOSENSOR BASED ON QUARTZ CRYSTAL MICROBALANCE
Bu çalışmada, alfa-fetoprotein AFP genine özgü tek zincirli oligonükleotit dizisinin belirlenmesine yönelik olarak geliştirilen, kuartz kristal mikrobalans KKM sistemine dayalı bir gen sensörü anlatılmıştır. Kuartz kristaller, poli etilenimin PEI polimeri ile kaplanmış, ardından glutaraldehit GA ile çapraz bağlama işlemi gerçekleştirilmiştir. Oligonükleotit probu, hazırlanan PEI/GA yüzeyi üzerine immobilize edilmiştir. Yüzeylerin kaplanması atomik kuvvet mikroskobu ile doğrulanmıştır. Tamamlayıcı ve prob dizileri arasında gerçekleşen hibridizasyon reaksiyonu, KKM sistemi ile eş-zamanlı olarak görüntülenmiştir. Bu çalışma ile, KKM sensörünün, kristal yüzeyi üzerinde gerçekleşen hibridizasyon reaksiyonunu, herhangi bir işaretleyici kullanmadan, eş-zamanlı olarak belirleyebildiği gösterilmiştir.
BASED ON QUARTZ CRYSTAL MICROBALANCE
In this study, a genosensor based on quartz crystal microbalance QCM has been developed for the detection of single-stranded oligonucleotide specific to alpha-fetoprotein AFP gene. The quartz crystals were coated with polyethyleneimine PEI and then crosslinked with glutaraldehyde GA . The oligonucleotide probe was immobilized onto the PEI-GA film. The coatings were characterized by using atomic force microscopy. Hybridization reaction between probe and the complementary strand was monitored by the QCM system in real-time. This work demonstrated that QCM is a suitable tool for the real-time monitoring of hybridization on the crystal surface without using any label.
Keywords:
Piezoelectric Sensor, Alpha-fetoprotein, Quartz Crystal Microbalance, Resonance Frequency, Atomic Force Microscopy Hybridization, Gene Sensor,
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- ISSN: 1303-6017
- Başlangıç: 1948
- Yayıncı: Ankara Üniversitesi