Sumru Sozer Karadagli, Borte Agrap, Ferzan Lermioglu Erciyas

Cinnamomum cassia kabuğu su ekstresinin sitotoksik ve genotoksik potansiyelinin araştırılması

Amaç: Son zamanlarda, tarçın ile ilgili çalışmalar zengin polifenol içeriğine bağlı antioksidan aktivitesi üzerinde yoğunlaşmıştır. Polifenollerin aynı zamanda pro-oksidan olarak hareket ettikleri ve DNA’da oksidatif zincir kırıklarına neden oldukları bildirilmiştir. Bu çalışmada Cinnamomum cassia su ekstresinin insan periferal kan lenfositlerindeki sitotoksik ve genotoksik potansiyelini araştırdık. Yöntemler: Cinnamomum cassia su ekstresi, toz haline getirilmiş tarçın kabuğunun ultra saf suda 72 saat maserasyonu ile hazırlandı. Ekstrenin lenfositlerdeki sitotoksik etkisi WST-1 yöntemi ile araştırıldı. Ekstrenin DNA’da hasar oluşturucu potansiyelini değerlendirmek için alkali Komet yöntemi uygulandı. DNA hasarı kuyruk DNA yüzdesi ve kuyruk momenti şeklinde ifade edildi.Bulgular: Lenfositlerin canlılığı ekstre ile 24 saat muamele sonrası konsantrasyona bağımlı olarak azaldı. Ekstre ?400 µg/ml konsantrasyonlarda negatif kontrole göre anlamlı düzeyde DNA hasarına neden oldu. Sonuç: Çalışma sonuçlarımız Cinnamomum cassia kabuğu su ekstresinin in vitro olarak sitotoksik ve genotoksik etki potansiyelini göstermektedir. Ekstrenin özellikle yüksek dozlarda ya da uzun süreli kullanımında güvenliliği açısından bu bulgu önemli görünmektedir ve bu nedenle in vivo çalışmalarla aydınlatılmalıdır.Anahtar Kelimeler : Cinnamomum cassia, alkali Komet yöntemi, sitotoksisite, pro-oksidan aktivite

Anahtar Kelimeler:

Cinnamomum cassia, alkali Komet yöntemi, sitotoksisite, pro-oksidan aktivite

Investigation of cytotoxic and genotoxic potential of Cinnamomum cassia bark water extract

Objective: Recently many investigations on cinnamon have focused on its powerful antioxidant activity due to its rich polyphenol content. Polyphenols have also been reported to act as pro-oxidants, causing oxidative strand breaks in DNA. In the present study, we investigated the cytotoxic and genotoxic potential of Cinnamomum cassia water extract in human peripheral blood lymphocytes. Methods: Cinnamomum cassia water extract was prepared from grounded bark of cinnamon by maceration with ultrapure water for 72 h. Cytotoxicity of the extract on lymphocytes was determined by WST-1 assay. Alkaline Comet assay was conducted to evaluate the DNA damaging potential of the extract. DNA damage was expressed as DNA percentage in the tail and tail moment. Results: The viability of lymphocytes was decreased by treatment with the extract for 24 h, as a concentration-dependent manner. At the concentrations ?400 µg/ml, the extract induced significant DNA damage compared to negative control.Conclusion: Our results show the cytotoxic and genotoxic potential of Cinnamomum cassia bark water extract in vitro. This finding seems a significant safety concern, particularly in high doses or long term use of the extract, and therefore needs to be clarified by in vivo studies.Key words: Cinnamomum cassia, alkaline Comet assay, cytotoxicity, pro-oxidant activity

Keywords:

Cinnamomum cassia, alkaline Comet assay, cytotoxicity, pro-oxidant activity,

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