Ezgi YAPRAK SÖYÜK, İBRAHİM SÖĞÜT, ESRA ÇİKLER DÜLGER, Canan HÜRDAĞ

Kronik Sudan Kaçınma Stresi ile Oluşturulan Oksidatif Hasar ve Fulvik Asidin Erkek Sıçan Dokusunda Erektil Disfonksiyon Üzerine Etkisi

Amaç: Çalışmamızda fulvik asitin antioksidan özelliğinden yararlanarak erektil disfonksiyon ile bağlantılı olan penil doku hasarın üzerine olan etkisini amaçladık. Gereç ve Yöntem: Çalışmamızda 18 adet Sprague-Dawley (250-300gr) erişkin erkek sıçan 3 eşit gruba ayrıldı: Kontrol (K), Kronik Stres (KS) ve Kronik Stres+Fulvik Asit (KS+FA). Penis doku kesitleri Hematoksilen&Eozin (H&E) ve Masson trikrom ile histokimyasal; endoteliyal; nöronal nitrik oksit sentaz (eNOS;nNOS), Kaveloin-1, immünohistokimya işaretlemeler incelenmiştir. Biyokimyasal olarak dokulardaki total antioksidan seviyesi (TAS), total oksidan seviyesi (TOS), oksidatif stres indeksi (OSİ), katalaz (KAT), glutatyon peroksidaz (GPx) ve süperoksit dismutaz (SOD) düzeyleri ölçülmüştür. Bulgular: KS grubu incelendiğinde kontrol grubuna kıyasla trabeküler endotel hücrelerinde kayıp, kollajen liflerde azalmalar ve incelmeler, bağ dokusu ve düz kas dağılımında bozulmalar gözlenmiştir. KS+FA grubu incelendiğinde KS grubuna kıyasla oluşan hasarlarda tam bir iyileşme gözlenmemekle beraber dokularda yer yer düzelmeler rastlanmıştır. KS grubunda eNOS ve nNOS aktivitesinin kontrol grubuna göre azalmış, kaveolin-1 reaksiyonun ise artmış olduğu gözlenmiştir. Aynı grupta TOS ve OSI değerlerinin arttığı, TAS, SOD, KAT ve GPx değerlerinin de azaldığı görülmüştür. KS+FA grubunda fulvik asit kullanımı ile kontrol grubuna yakın olduğu gözlenmiştir. Sonuç: Kullanılan fulvik asitin tedavi edici etkileri olduğu fakat tedavi için kullanılan dozun yeterli miktarda olmadığı saptanmıştır. Tedavi edici etkinin yeterli miktarda olması için farklı dozlarda çalışılmasını ileri sürmekteyiz.

Anahtar Kelimeler:

Erektil disfonksiyon, Sudan kaçınma stresi, Oksidatif stres, İnfertilite, Fulvik asit

Oxidative Damage of Chronic Water Avoidance and the Effect of Fulvic Acid in Male Rat Tissues with Erectile Dysfunction

Objectives : We aimed to utilize the antioxidant property of fulvic acid in our study to prevent penile tissue damage associated with erectile dysfunction.Material and Methods: 18 Sprague-Dawley male adult rats were distributed into 3 experimental groups: Control (C), Chronic Stress (CS) and Water Avoidance Stress+Fulvic Acid (CS+FA). The histological sections obtained from the testes has been stained with the Hematoxylin&Eosin (H&E) and Masson Trichrome and endothelial and n nitric oxide sentase (eNOS &nNOS) immünohistochemistry labelling has been made to. Total antioksidant status (TAS), total oksidant status (TOS), oxidative stress (OSI), catalase (CAT), glutation peroksidase (GPx) and superoxide dismutase (SOD) levels has been evaluated biochemically. Results: In CS group were examined, it was observed that trabecular endothelial cells are lost, collagen fibers had shrunk, connective tissue and smooth muscle distributions were unevenly distributed compared to the control group. When CS + FA group were examined, damage in the CS group showed considerable recovery in tissues that were treated. In CS group both eNOS and nNOS reactivity had decreased compare to the control group whereas caveolin-1 reactivity had increased. histochemical data. It was also observed increased TOS and OSI levels and decreased TAS, SOD, CAT and GPx levels in the KS group. Conclusion: The fulvic acid used was found to have therapeutic effects but the dosage used for treatment was not sufficient. We suggest to work at different but higher dosages to ensure that the therapeutic effect is achieved.

Keywords:

Erectile dysfunction, water avoidance stress, oxidative stress, infertility, fulvic acid,

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