OKTAY ÖZKAN, CANER ÖZBEY, IŞIL AYDEMİR, İSMAİL SARI, GONCA DÖNMEZ ARAT, Fatma Esi̇n KIRIK, Ahmet SAVRAN, MEHMET İBRAHİM TUĞLU

Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması

Amaç: Kanser, doku ve organları istila eden ve vücudun diğer kısımlarına sıçrayabilen, hücrelerin anormal gelişimidir. Meme kanseri en çok tekrarlanan tanıya sahip ve dünya genelinde kadınlarda kanserle ilişkili ölümlerde en önde gelen sebeplerdendir. Tıbbi bitkiler bazı kanser türlerinde antikarsinojenik özelliklere sahip olabilmektedir. Bu çalışmanın amacı Origanum minutiflorum O. Schwarz & P.H. Davis’in in vitro şartlarda, meme kanseri hücre dizilerinde sitotoksik etkisini araştırmaktır. Gereç ve Yöntem: Bu çalışmada, MDA-MB-231 ve MCF-7 meme kanseri hücre dizileri ve adipoz dokudan izole edilmiş mezenkimal kök hücreleri kullanıldı. Origanum minutiflorum’dan elde edilmiş esansiyel yağa maruz bırakılmış tüm hücre dizilerinde MTT testi aracılığıyla IC50 dozu hesaplandı. Her bir hücre dizisinin IC50 dozunda eNOS, p53, PCNA ve TUNEL için immunositokimya boyaması yapıldı. Sonuçlar Graphpad yazılımı kullanılarak one-way-ANOVA kullanılarak değerlendirildi. Bulgular: Meme kanseri hücrelerinin proliferasyonu oragano yağı tarafından inhibe edildi. Oregano yağı mezenkimal kök hücrelerinde herhangi bir sitotoksik etki göstermedi. eNOS ve p53 ile apoptozisin göstergesi TUNEL’de artış yoluyla oreagono yağının kanser hücrelerinde sitotoksik olduğu ortaya çıktı ve PCNA boyamada proliferasyonun azaldığı tespit edildi. Sonuç: Bizim verilerimize göre oregano yağı MDA-MB-231 ve MCF-7 meme kanseri hücrelerinde sitotoksik bir etkiye sahiptir ve diğer biyolojik aktiviteleri için aktif komponentlerini tanımlayacak testlere ihtiyaç vardır.

Anahtar Kelimeler:

Meme kanseri, MCF-7, MDA-MB-231, Origanum minutiflorum, sitotoksisite, oksidatif stres

Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells

Objective: Cancer is defined by a abnormal growth of cells, that invade tissues and organs and spread to other parts of the body. Breast cancer is one of the most repeatedly diagnosed cancers and the foremost cause of death among women worldwide. Medicinal plants can have an anticancerogenic effect in some cancer types. Our aim is to search the cytotoxic effect of Origanum minutiflorum O. Schwarz & P.H. Davis on breast cancer cells in vitro conditions. Methods: In this study, MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived mesenchymal stem cells were used. Essential oil from Origanum minutiflorum was exposed to the all cells and IC50 dose was calculated by MTT assay. We have examined immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each cells. The results were evaluated using one-way-ANOVA by Graphpad software. Results: The proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil did not show any cytotoxic effect in mesenchymal stem cells. It was determined that cytotoxic effect of oregano oil on cancer cells was occurred through increase of eNOS and p53 staining for survival and the TUNEL labeling for apoptosis, and decreased of PCNA staining for proliferation. Conclusion: According to our data, oregano oil has cytotoxic effect on MDA-MB-231 and MCF-7 breast cancer cell lines, and it needs to be analysis to detect active components for other biological activities.

Keywords:

Breast cancer; MCF-7; MDA-MB-231; Origanum minutiflorum; cytotoxicity; oxidative stress; apoptosis,

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