Yonca YÜZÜGÜLLÜ, Halil TOYGAR, Arda ACEMİ, Fazıl ÖZEN, Yonca Avcı DUMAN

Tuz Stresi ve BAP Varlığında Amsonia orientalis’in Antioksidan Enzimlerinin İncelenmesi

Amsonia orientalis Decne. (Apocynaceae), dünyada sadece Türkiye'nin kuzeybatısı ve Yunanistan'ın kuzeydoğusunda yayılış gösteren, tıbbi ve ekonomik öneme sahip yapısında çok sayıda glikozitler ve glikoalkaloitler bulunduran, nadir ve tükenme tehdidi altında olan bir bitki türüdür. Bitkinin tükenme tehlikesi altına girişinin önemli sebeplerinden biri olarak giderek artan abiyotik stres koşulları düşünülmektedir. Çalışmamızda, tuzluluk stresinin Amsonia orientalis'in H2O2 ve MDA seviyeleri ile antioksidan enzimlerinin (SOD, CAT, POD) üzerindeki etkisi; stresi yatıştırmak amacıyla 1,0 mg/mL benzilaminopürin (BAP) varlığında elektrofotometrik ve fotometrik olarak irdelelenmiştir. Artan tuz stresi koşullarında H2O2 ve MDA seviyelerinin benzer olarak yüksek tuz konsantrasyonlarında arttığı ancak düşük tuz konsantrasyonlarında azaldığı; üç antioksidan enzimin de spesifik aktivitelerinin arttığı gözlenmiştir. Elektrofotometrik analizler sonucunda artan tuz stresinin bitkinin Fe-SOD ve Cu/Zn-SOD aktivitelerini inhibe ettiği, Mn-SOD aktivitesinde değişiklik oluşturmadığı, CAT ve POD aktivitelerinin ise tüm tuz konsantrasyonlarında korunduğu gözlenmiştir

Investigation of Antioxidant Enzymes of Amsonia orientalis in the Presence of Salt Stress and BAP

Amsonia orientalis Decne (Apocynaceae), is a threatened plant species providing many glycosides and glycoalkaloids which have medicinal and economic significance. The species has a natural distribution only in the northwest of Turkey and the north-east of Greece. Abiotic stress factors are tought to be major causes of plant limited distribution. . Thus, the effect of the increasing salt concentrations onMDA and H2O2 content and antioxidant enzymes (SOD, CAT, POD) of Amsonia orientalis was investigated in the presence of 1.0 mg/mL benzylaminopurine (BAP)-due to sooth effect of BAP- spectrophotometrically and electroprotometrically. It was found that salt stres decreased to H2O2 contend but elevated at high concentrations of NaCl and enhanced the specific activities of antioxidant enzymes tested. Electrophotometric analysis showed that Fe-SOD and Cu/Zn-SODs activities were inhibited while Mn-SOD, CAT and POD activities remained their activities against increasing salt stres

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