Oluşturucu Ajanın Boyutu ve Bağlantı Süresinin Etkileri

Bu çalışmada, makrogözenekli ve gözenekleri birbirleri ile bağlantılı olarak kalıplama, ısı etkili faz ayrımı ve tanecik uzaklaştırma yöntemleri ile üretilen jelatin bazlı doku iskelelerinde gözenek oluşturucu ajan olan parafin kürelerin boyutu ve bağlantı sürelerinin doku iskelelerinin mekanik özellikleri üzerine olan etkileri incelenmiştir. Sünger formlu ve açık hücreli yapıda olan doku iskelelerinin mekanik özellikleri sıkıştırma testi ile ölçülmüştür. Yapılan ölçümler parafin küre çapındaki artışın doku iskelelerinin sıkıştırma katsayısını istatistiksel olarak anlamlı bir şekilde etkilemediğini göstermiştir. Ancak, parafin kürelerin maruz bırakıldığı ısı etkileşim süresinin 100 dakikadan 400 dakikaya arttırılmasının 250-425 µm çapındaki parafin kürelerle hazırlanan doku iskelesinin elastisite değerini arttırdığı belirlenmiştir (p

The Effects of Porogen Agent Size and Interconnection Time on the Mechanical Properties of Gelatin Scaffold

In this study, the effects of particle size and interconnection time of porogen agent paraffin spheres on the mechanical properties of macroporous and interconnected scaffolds prepared with combination of molding, phase separation and particle leaching methods were investigated. The mechanical properties of the open-cell foam type scaffolds were measured with a compressive test. It was determined that the increase in the size of paraffin spheres did not significantly change the compressive modulus of the scaffolds. On the other hand, elasticity of the scaffold prepared from 250-425 µm sized paraffin spheres increased significantly (p

PDF

___

Subia, B.; Kundu, J.; Kundu, S.C. Biomaterial scaffold fabrication techniques for potential tissue engineering appli- cations. In Tissue Engineering; Eberli, D., Ed.; In Tech: Croa- tia, 2010; 141-157.
Ma, P.X.; Langer, R. Fabrication of Biodegradable Poly- mer Foams for Cell Transplantation and Tissue Engineering. In Tissue Engineering Methods and Protocols; Morgan, J., Yarmush, M., Eds.; Humana Press: New Jersey, 1993; 47-56.
Lu, L.; Peter, S.; Lyman, M.; Lai, H;, Leite, S.; Tamada, J.; Uyama, S.; Vacanti, J.; Langer, R.; Mikos, A. In vitro and in vivo degradation of porous poly (DL-lactic-co-glycolic acid) foams. Biomaterials. 2000, 21, 1595-1605.
Park, S.; Kim, G.; Jeon, Y.C.; Koh, Y.; Kim, W. 3D polycaprolactone scaffolds with controlled pore structure using a rapid prototyping system. J. Mater. Sci. Mater. Med. 2009, 20, 229-234.
Chan, B.P.; Leong, K.W. Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur. Spine. J. 2008, 17, (Suppl 4) S467-S479.
Discher, D.E.; Janmey, P.; Wang, Y.L. Tissue cells feel and respond to the stiffness of their substrate. Science. 2005, 310, 1139-1143.
Engler, A.J.; Sen, S.; Sweeney, H.L.; Discher, D.E. Matrix elasticity directs stem cell lineage specification. Cell. 2006, 126, 677-689.
Liu, X.; Ma, P.X. Phase separation, pore structure, and properties of nanofibrous gelatin scaffolds. Biomaterials. 2009, 30, 4094-4103.
Ma, P.X.; Choi, J-W. Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. Tissue Eng. 2001, 7, 23-33.
Wang, T.W.; Spector, M. Development of hyaluronic acid-based scaffolds for brain tissue engineering. Acta Biomater. 2009, 5, 2371-2384.
Ahlin, P.; Kristl, J.; Kristl, A.; Vrecer, F. Investigation of polymeric nanoparticles as carriers of enalaprilat for oral administration. Int. J. Pharm. 2002, 239,113-120.
Bolourtchian, N.; Karimi, K.; Aboofazeli, R. Preparation and characterization of ibuprofen microspheres. J. Microencapsul. 2005, 22, 529-538.
Francis, L.; Meng, D.; Knowles, J.; Keshavarz, T.; Boccaccini, A.R.; Roy, I. Controlled delivery of gentamicin using poly(3-hydroxybutyrate) microspheres. Int. J. Mol. Sci. 2011, 12, 4294-314.
Chen, V.J.; Ma, P.X. Nano-fibrous poly(L-lactic acid) scaffolds with interconnected spherical macropores. Bio- materials. 2004, 25, 2065-73.
Kim, H.Y.; Kim, H.N.; Lee, S.J.; Song, J.E.; Kwon., S.Y.;
Chung, J.W.; Lee, D.; Khang, G. Effect of pore sizes of PLGA
scaffolds on mechanical properties and cell behaviour for
nucleus pulposus regeneration in vivo. J. Tissue. Eng. Regen.
Med. 2014, [Epub ahead of print].
Sartori, S.; Chiono, V.; Tonda-Turo, C.; Mattu, C.;
Gianluca C. Biomimetic polyurethanes in nano and regen
erative medicine. J. Mater. Chem. B. 2014, 2, 5128-5144.