Fungal ve Arkeal Kaynaklı Lipazların Eldesi, Aktivitelerinin Kıyaslanması ve Kağıt Üretiminde Kullanımı
Lipaz,
gıda teknolojisi, klinik ve endüstriyel kimya gibi birçok biyoteknolojik alanda
yaygın olarak kullanılan bir enzimdir. Endüstriyel alanda kullanılan lipazların
çoğu funguslardan elde edilir. Bu enzimler ticari olarak satılmaya uygun olup
saflaştırılır ve immobilize hale getirilir. Ancak tüm bu işlemler ürünün
maliyetini arttırdığından son ürün haline dönüşen lipaz enzimi oldukça
pahalıdır. Kağıt üretiminde karşılaşılan problemlerin en önemlilerinden biri
odun ekstraktiflerinin kağıt hamuru ve kağıtta kaliteyi düşürmesi ve atık
sularda toksisite oluşturmasıdır. Bu yapışkan bileşiklerden biyolojik yolla
kurtulmak amacıyla lipaz enzimi kullanılabilir. Bu çalışmada, çürükçül bir
fungus olan Phanerochaete chrysosporium
ile bir halofilik arke olan Haloarcula
hispanica’dan elde edilen ekstraselüler lipazların aktivite tayini yapılmış
ve geri dönüşümlü karton üretimi proseslerinden alınan kağıt hamuru numunelerine
uygulanmıştır. Aseton ekstraksiyonuna tabi tutulan kağıt hamuru numunelerine
nem analizi yapıldıktan sonra lipaz eklenmiştir. Hidrolizi daha detaylı ölçmek
amacıyla asit değeri analizi yapılarak serbest yağ asidi miktarını belirlemek
amacıyla gaz kromatografi uygulanmıştır. Sonuçlar, P. chrysosporium ve H. hispanica’dan
düşük maliyetle elde edilen lipaz enzimlerinin herhangi bir saflaştırma veya
immobilizasyon yapılmaksızın aktivite gösterdiğini ve lipofilik bileşiklerin
hidrolizinde etkin biçimde kullanılabileceğini göstermiştir.
Anahtar Kelimeler:
Haloarcula hispanica, Kağıt hamuru, Lipaz, Lipofilik bileşiklerin hidrolizi, Phanerochaete chrysosporium
Production of Fungal and Archaeal Lipases, Comparison of their Enzymatic Activities and Utilization in Paper Production
Lipases are used extensively in biotechnological
fields such as food technology, clinical and industrial chemistry. Many
industrially used lipases are prepared from fungi. Hence, their consideration
as industrially relevant enzymes includes purification and immobilization
processes which means an increase in the price of the final product. One of the
most important problems in paper production is wood extractives so-called pitch
that cause low quality pulp and paper, and create waste water toxicity. Lipase could
be used for the hydrolysis of those sticky compounds as a biological approach.
In this study, extracellular lipases of a fungus Phanerochaete chrysosporium and a halophilic archaeon Haloarcula hispanica were used in pitch
component and sticky material degradation in recycled paper production in
comparison with controls. Acetone extraction was applied on the samples. After
the determination of the enzyme activity lipases were added to the pulp
samples. The amount of free fatty acids were determined by acid value analysis
in order to get a better quantification of the hydrolysis. Also gas
chromatography was applied. The results demonstrated that P. chrysosporium and H.
hispanica extracellular
lipases have substantially high enzyme activity without any purification or
immobilization processes and can be efficiently used for the hydrolysis of
lipophilic compounds in an environmentally friendly approach.
Keywords:
Haloarcula hispanica, Hydrolysis of lipophilic compounds, Lipase, Paper pulp, Phanerochaete chrysosporium,
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- ISSN: 1305-130X
- Başlangıç: 2005
- Yayıncı: Manisa Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü