Effects of Detergents, Ions, and Organic Solvents on the Activity of Four Bacillus clausii Pectinases

Four different pectinolytic enzyme activity essays were employed to detect different pectinases of Bacillus clausii from an extracellular protein cocktail. The mixture was obtained by ethanol precipitation. Each of the essays produced a positive result, indicating the presence of pectate lyase (PGL), pectin lyase (PL), pectin methyl esterase (PME), and polygalacturonase (PG). Activities of these enzymes were then assessed at differing concentrations of NaCl, detergents (SDS and tweens), ion chelators, and organic solvents. Results, presented as histograms, were the indication of original findings, as most of them were new to the relevant literature.

Keywords:

Bacillus clausii, ionic strength tolerance, organic solvent tolerance, pectinase activity, salt tolerance,

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[1] Kashyap, D.R., Vohra, P.K. Chopra, S. and Tewari, R. 2001. Applications of Pectinases in the Commercial Sector: A Review”, Bioresource Technology; 77: 215-227.
[2] Hoondal, G.S., Tiwari, R.P., Tewari, R., Dahiya, N. and Beg, Q.K. 2002. Microbial Alkaline Pectinases and Their Industrial Applications: A Review. Applied Microbiology Biotechnology; 59: 409-418.
[3] Sharma, N., Rathore, M. and Sharma, M. 2012. Microbial Pectinase: Sources, Characterization and Applications. Reviews in Environmental Science and Biotechnology; 12: 45-60.
[4] Al-Rajabi, I.I. and Mahasneh, A.M. 1999. Partial Characterization of an Alkalophilic Extracellular Crude Pectinases From a Bacillus polymyxa Strain. Qatar University Science Journal; 18: 67-80.
[5] Kashyap, D.R., Chandra, S., Kaul, A. and Tewari, R. 2000. Production, Purification and Characterization of Pectinase from a Bacillus sp. DT7. World Journal of Microbiology and Biotechnology; 16: 277-282.
[6] Joo, H.S., Kumar, C.G., Park, G.C., Paik, S.R. and Chang, C.S. 2003. Oxidant and SDS-stable alkaline protease from Bacillus clausii I-52: Production and Some Properties. Journal of Applied Microbiology; 95: 267-272.
[7] Mei, Y., Chen, Y., Zhai, R. and Liu, Y. 2013. Cloning, Purification and Biıchemical Properties of a thermostable Pectinase from Bacillus halodurans M29. Journal of Molecular Catalysis B: Enzymatic; 94: 77-81.
[8] Raju, E.V.N. and Divakar, G. 2013. Production of Pectinase by Using Bacillus circulans Isolated From Dump Yards of Vegetable Wastes. IJPSR; 4: 2615-2622.
[9] Sassi, A.H., Tounsi, H., Trigui-Lahiani, H., Bouzouita, R., Romdhane, Z.B. and Gargouri, A. 2016. A Low-Temperature Polygalacturonase from P.occitanis: Characterization and Application in Juice Clarification. International Journal of Biological Macromolecules; 91: 158-164.
[10] Madu, O.J., Okonji, R.E., Torimiro, N. and Agboola, F.K. 2016. Purification and Characterization of Pectinase from Bacillus licheniformis obtained from a Cassava Waste Dump. Journal of Advances in Biology; 8: 1685-1695.
[11] Urdaci, M., Bressollier, P. and Pinchuk, I. 2004. Bacillus clausii Probiotic Strains Antimicrobial and Immunodulatory Activities. Journal of Clinical Gastroenterology; 38: 86-90.
[12] Berber, S. 2018. Productıon, Isolatıon and Characterızatıon of Extracellular Pectinase from Soil Bacteria. Master's Thesis, Cumhuriyet University Institute of Science; Sivas, 21.
[13] Kocabay, S. 2015. Producdution, Isolation and Characterization of Extracellular Amylase Enzyme from Gaıta Bacreria. Master's Thesis, Cumhuriyet Üniversitesi Fen Bilimleri Enstitüsü; Sivas, 96.