İbrahim Ender KÜNİLİ, Fatma Arık ÇOLAKOĞLU

Deniz Hıyarı (Holothuria tubulosa, Gmelin 1791) Ekstraktlarının Antioksidan ve Antimikrobiyal Aktivitesi

Holothuria tubulosa Türkiye denizlerinde bulunan ekonomik ve en çok hasat edilen deniz hıyarı türlerindendir. Deniz hıyarları insan sağlığı için önemli olan bioaktif bileşiklerin kaynağı olarak bilinmektedir. Bu çalışmada H. tubulosa’nın antioksidan ve antimikrobiyal etkisinin belirlenmesi amaçlanmıştır. H. tubulosa ekstraktları, asitontril/trifloroasetik asit, methanol ve su/metanol gibi farklı çözücüler kullanılarak elde edilmiştir. Sonuçlara göre, ekstraktların antioksidan aktiviteleri, referans antioksidanlara göre tüm konsantrasyonlarda daha düşük belirlenmiştir. Ancak, yüksek konsantrasyonlarda, ekstraktların antioksidan aktivitesi referans antioksidanlara yakın derecede bulunmuştur. Bakteriyostatik etki 12 µg/ml konsantrasyonundan sonra belirlenmiş, ancak bu etki hiçbir konsantrasyon ve ekstrakta, Bacillus cereus için gözlemlenmemiştir. Sonuç olarak, Türkiye denizlerinde bulunan H. tubulosa’nın, potansiyel doğal antioksidan kaynağı olabileceği düşünülmektedir.

Anahtar Kelimeler:

Holothuria tubulosa, Deniz Hıyarı Ekstraktı, Antimikrobiyal Aktivite, Antioksidan Aktivite, DPPH

Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts

Holothuria tubulosa is an economic and most harvested sea cucumber species found in Turkey seas. Sea cucumbers are known to be sources of some of bioactive components which are play important roles in human health. In this study, it was aimed to determine the antioxidant and antimicrobial activity of H. tubulosa. The extracts of H. tubulosa were obtained with using different solvents such as, acetonitrile/trifloroacetic acid, methanol, and water/methanol. According to results, antioxidant activity of the extracts was lower than the reference antioxidant agents at all concentrations. However, the activity at higher concentrations of extracts was found to be closer to reference agents. Bacteriostatic effect was observed after the concentration of 12 µg/ml for all bacteria except Bacillus cereus, which has also shown resistance to reference antibiotic. As a consequence, H. tubulosa found in Turkey seas may be regarded as potential source of natural antioxidant agent.

Keywords:

Holothuria tubulosa, sea cucumber extract, antimicrobial activity, antioxidant activity, DPPH,

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