Ayşegül KUMRAL, Nabi Alper KUMRAL, Ozan GÜRBÜZ

Candida boidinii’nin Farklı Suşlarının Deltamethrini Parçalama Potansiyellerinin İn-vitro Koşullarda Belirlenmesi

Bitkisel üretimde kullanılan pestisitlerin gıdalarda kalıntılarının azalmasını etkileyen önemli faktörlerden biri de mikrobiyal faaliyetlerdir. Bu çalışmada, zeytin fermentasyonu salamurasından izole edilen Candida boidinii’nin yedi farklı suşunun bitkisel üretimde sıkça kullanılan sentetik piretroitli insektisit deltamethrin’i parçalama potansiyeli invitro koşullarda incelenmiştir. Bu amaçla, maya hücrelerinin esteraz enzim aktiviteleri ve deltamethrini karbon kaynağı olarak değerlendirme durumları tespit edilmiştir. Esteraz enzim aktivitesi ve deltamethrini karbon kaynağı olarak değerlendirme potansiyelleri yüksek olan suşların insektisiti parçalama düzeyleri gaz kromatografisi kütle spektrofotometresi (GC-MS) kullanılarak belirlenmiştir. Test edilen yedi suşun ikisinde (CB-1 ve CB-5) hem hücre gelişimi hem de esteraz aktivitesi açısından önemli düzeyde yüksek faaliyet belirlenmiştir. GC-MS kalıntı analizine göre, iki suşun ilk üç günde deltamethrin miktarında başlangıca göre sırasıyla % 41.4 ve 22.5 oranında azalmaya sebep olduğu, maya bulunmayan kontrol grubunda ise bu oranın sadece %11 olduğu tespit edilmiştir. CB-5 suşunda 10 gün içinde önemli düzeyde daha fazla deltamethrin yıkımı (%91) belirlenmiştir. Elde edilen sonuçlara göre C. boidinii’nin farklı suşlarının deltamethrinin parçalanmasında rol oynayabileceği yönünde güçlü kanıtlar ortaya konmuştur.

Anahtar Kelimeler:

Deltamethrin, esteraz, insektisitler, mayalar, parçalanma, sentetik piretroitler

Determination of Deltamethrin Degradation Potentials of Different Candida boidinii Strains in In-vitro conditions

Microbial activities are one of the important factors affecting the reduction of the residues of pesticides used during plant production in foods. In this research, the potential of seven strains of Candida boidinii previously isolated from olive fermentation brines for the degradation of synthetic pyrethroid insecticide deltamethrin, commonly used in crop production, was investigated under in-vitro conditions. For this purpose, esterase enzyme activities of yeast cells and evaluation of deltamethrin as a carbon source were determined. Esterase enzyme activity and insecticide degradation levels of strains with high potential to evaluate deltamethrin as a carbon source were determined by using gas chromotography mass spectrophotometer (GC-MS). Two of the seven strains (CB-1 and CB-5) were displayed significant high activity both in terms of cell development and esterase activity. According to the GC-MS residue analysis, it was determined that the two strains caused a 41.4% and 22.5% decrease in the amount of deltamethrin in the first three days, respectively, compared to the initial levels, while this ratio was only 11% in the non-yeast control group. Significant further deltamethrin degradation (91%) was determined within 10 days in CB-5 strain. According to the results obtained, strong evidence has been revealed that the different strains of C. boidinii may play a role in the degradation of the deltamethrin.

Keywords:

deltamethrin, esterase, insecticides, yeasts, degradation, synthetic pyretriods,

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