Osman ÇAĞLAYAN, İdris MEHMETOĞLU, Ümmügülsüm CAN

TİP 1 DİYABET MEİLLİTUS’DA SERUM. LÖKÖSİT VE ERİTROSİT LİPİD PEROKSİDASYONU VE ANTİOKSİDAN DURUM Investigation of Serum, Leukocyte and Erythrocyte Lipid Peroxidation and Antioxidative Defence Systems in Type 1 Diabetes Mellitus

Giriş: Tip 1 diyabet mellitus metabolik bir hastalık olup, patogenezinde oksidatif stres rol oyna-maktadir. Oksidatif stres serbest oksijen radikalleri ve lipid peroksidlerinin artışı ve antioksidankapasitesinin azalmasına bağlıdır. Bu çalışmada tip 1 diyabetmellitus’da oksidatif stresin etkisini değerlendirmek için serum, lökösit ve eritrosit lipid perok-sidasyonu, ve lökösit ve eritrosit glutatyon peroksidaz ve süperoksid dizmutaz aktivitelerineilaveten serum ve lökösit vitamin C seviyelerini sağlıklı kontroller ile karşılaştırdık.Yöntem: Çalışmaya hasta grubu olarak 34 çocuk ve adolesan (20 kız ve 14 erkek) ve kontrol gru-bu 29 çocuk ve adölesan (13 kız ve 16 erkek) dahil edildi. Lipid peroksidasyonunun son ürünümalondialdehid, tiyobarbitürik asid reaktivite yöntemi, vitamin C seviyeleri dinitrofenilhidrazinmetodu ile ölçüldü. Glutatyon peroksidaz ve süperoksid dizmutaz düzeyleri ticari kit kullanıla-rak tesbit edildi. Analiz sonuçlarının istatiksel analizleri SPSS 20.0 bilgisayar proğramı ile yapıldı.Bulgular: Tip 1 diyabet mellitus çocuk ve adölesanlar kontroller ile karşılaştırıldığında serum,lökosit ve eritrosit malondialdehid seviyeleri artmasına karşın lökosit ve eritrosit glutatyon pe-roksidaz ve süperoksid dizmutaz aktiviteleri, ve serum ve lökosit vitamin C seviyelerinde azalmatesbit edilmişti. Fakat bu değişikliklerde istatistiksel olarak anlamlı fark gözlenmedi (p > 0.05).Bu parametreler ile HbA1c ve glukoz seviyeleri arasında korelasyon yoktu.Sonuç: Tip I diyabet mellitus cocuk ve adolesanlarda lipid peroksidasyon artışına ve antioksidansisteminde azalmaya eğilim olmakla birlikte bu durumun düzenli kullanılan insülin tedavisi iledüzeltildiği ve diğer faktörler tarafında etkilendiği kanaatindeyiz.

Investigation of Serum, Leukocyte and Erythrocyte Lipid Peroxidation and Antioxidative Defence Systems in Type 1 Diabetes Mellitus

Background & objectives: In the present study, to evaluate oxidative stress in type 1 diabetes mellitus (IDDM), serum, leukocyte and erythrocyte lipid peroxidation and and erythrocyte and leukocyte glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities, and serum and leukocyte vitamin C levels of patients and healthy controls were investigated in order to determine the effect of free radicals in these patients. Lipid peroxidation was measured in terms of malondialdehyde which is an end product of peroxidation. Methods: Patients consisted of 34 cases (20 female,14 male) aged 3-27 years and controls consisted of 29 subjects (13 female, 16 male) aged 3-18 years. Results: There was at least, a tentency toward an increase in lipid peroxidation and a decrease in antioxidant systems in children and adolescents with IDDM which is corrected by insülin treatment. Additionally, there was no correlation between the above parameters and HbA1c and glucose levels. Conclusions: These disturbances were concluded to be due to some other factors rather then diabetes itself.

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