MEZENTERİK İSKEMİ-REPERFÜZYONLA İNDÜKLENEN İNTESTİNAL DOKU HASARINA KARŞI EVODİAMİN’İN ETKİSİ: OKSİDATİF STRESİN ROLÜ

Amaç: Bu çalışma, evodiaminin mezenterik iskemi-reperfüzyonun neden olduğu intestinal dokudaki oksidatif hasara karşı muhtemel yararlı etkisini göstermeyi amaçlamıştır. Materyal ve Metod: Bu amaçla çalışmamızda deney hayvanlarında mezenterik iskemi-reperfüzyon modeli yapıldı. İskemi-reperfüzyon ve evodiamin düşük ve yüksek doz tedavi gruplarında, superior mezenterik arter 1 saat süreyle klemplendi. Daha sonra klemp açıldı ve 2 saatlik reperfüzyon başlatıldı. Deney sürecinin sonlandırılmasından sonra, tüm hayvanlar sakrifiye edildi ve intestinal doku örnekleri toplandı. Bulgular: TOS, OSI, MDA düzeyleri ve MPO aktivitesinin sham grubuna göre mezenterik iskemireperfüzyon grubunda arttığı görüldü. ''Ayrıca mezenterik iskemi-reperfüzyon grubunda SOD aktivitesi ve TAS seviyesinin sham grubuna göre azaldığı tespit edildi. ''Düşük ve yüksek dozlarda evodiamin (10 ve 20 mg / kg) uygulanan gruplarda mezenterik iskemi-reperfüzyon grubuna göre TAS değeri ve SOD aktivitesi artarken, TOS, OSI değerleri, MPO aktivitesi ve MDA düzeyi azaldığı belirlendi. Sonuç: İki farklı evodiamin dozu mezenterik iskemi-reperfüzyonun neden olduğu intestinal dokunun oksidatif hasarına karşı yararlı etkiler göstermiştir

The Effect of Evodiamine Against Intestinal Tissue Injury Induced By Mesenteric Ischemia-Reperfusion: Role of Oxidative Stress

Purpose: This study aimed to show possible benefical effect of evodiamine against oxidativedamage of intestinal tissue induced by mesenteric ischemia-reperfusion.Materials and Methods: For this purpose, in our study mesenteric ischemia-reperfusion modelwas conducted in experimental animals. In low and high doses treatment of evodiamine andischemia-reperfusion groups, superior mesenteric artery was clamped for 1 h. Then, the clampwas opened and reperfusion was started for 2 h. After the termination of the experimentalprosses, all animals were sacrificed and intestinal tissue samples were collected.Results: It was seen that TOS, OSI, MDA levels and MPO activity increased in mesenteric ischemiareperfusion group compared to sham group. Moreover, SOD activity and TAS level reduced inmesenteric ischemia-reperfusion group compared to sham group. When compared to mesentericischemia-reperfusion group, TAS value and SOD activity increased while TOS, OSI values, MPOactivity and MDA level decreased in groups treated with low and high doses of evodiamine (10and 20 mg/kg).Conclusion: Two different doses of evodiamine revealed benefical effects against oxidativedamage of intestinal tissue induced by mesenteric ischemia-reperfusion.

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