DERİ KANSERİ HASTALARININ SERUM OKSİDATİF STRES PARAMETRELERİNİN DEĞERLENDİRİLMESİ
Giriş ve amaç: Deri kanserleri en sık görülen kanserlerden biridir. Bu kanserler non melanomve melanom olarak genellikle iki grup altında incelenir ve çoğu nonmelanomadır. Deri kanserlerinin insidansı ve mortalite oranları tüm dünyada giderek artmaktadır. Bu çalışmada hücre içiantioksidanlar olan superoksit dismutaz (SOD), katalaz (CAT), redükte glutatyon (GSH), glutatyonredüktaz (GR), glutatyon peroksidaz (GPx) gibi moleküller ile oksidatif stres belirteci olan malondialdehit (MDA) değerlerini deri kanseri hastalarında incelemek istedik.Materyal ve metod: Prospektif çalışma modifiye edilen Dünya Helsinki Deklarasyonu'na göreuygulandı. Bu çalışma üniversite hastanesinin dermatoloji departmanında yapıldı. Çalışmaya 30deri kanseri hastası ve 30 sağlıklı gönüllü alındı. Çalışmaya katılanların serumlarında hücre içiantioksidantlar ve malondialdehit seviyeleri değerlendirildiBulgular: Çalışma 33 erkek (%55), 27 kadın (%45) olmak üzere toplam 60 kişi ile gerçekleştirilmiştir. Çalışmaya katılan kişilerin hasta ve kontrol grubuna göre yaş ve cinsiyet değişkenlerikarşılaştırılmış aralarında anlamlı farklılık saptanmamıştır (p>0.05). GPx, GR, GSH, SOD, MDA,CAT değerlerinin, hasta ve kontrol grup ortalamaları arasındaki fark istatistik olarak önemli bulunmuştur (p
Evaluation of Serum Oxidative Stress Parameters of Skin Cancer Patients
Introduction and objectives: Skin cancers are one of the most common cancers. These cancers are usually examined under two groups as non-melanomas and melanomas, and most nonmelanomas are . The incidence and mortality rates of skin cancers are increasing all over the world. In this study, we want to investigated the intracellular antioxidants such as superoxide dismutase (SOD), catalase (CAT), reductase glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx) and malondialdehyde (MDA) values, which are oxidative stress markers, in skin cancer patients Material and method: The prospective study was carried out according to the modified World Helsinki Declaration. This study was done in the dermatology department of the university hospital. Thirty skin cancer patients and 30 healthy volunteers were included in the study. Intracellular antioxidants and levels of malondialdehyde were evaluated of participants in the study Results: The study consisted of 33 men (55%) and 27 women (45%). There was no significant difference between the age and gender variables of the study participants according to the patient and control group (p>0.05). The difference between GPx, GR, GSH, SOD, MDA, CAT values, patient and control group averages were statistically significant (p
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- Linares MA, Zakaria A, Nizran P. Skin Cancer. Prim Care.
2015;42(4):645-59.
- Marks R. An overview of skin cancers. Incidence and causation.
Cancer. 1995;15;75(2 Suppl):607-12.
- Basel H, Kavak S, Demir H, Meral I, Ekim H, Bektas H. Effect of levosimendan injection on oxidative stress of rat myocardium. Toxicol
Ind Health. 2013;29(5):435-40.
- Sander CS, Chang H, Hamm F, Elsner P, Thiele JJ. Role of oxidative
stress and the antioxidant network in cutaneous carcinogenesis. Int
J Dermatol. 2004;43(5):326-35.
- Kryston TB, Georgiev AB, Pissis P, Georgakilas AG. Role of oxidative stres and DNA damage in human carcinogenesis. Mutat Res.
2011;711(1-2):193-201.
- Waris G, Ahsan H. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog.
2006;5:14.
- Sorg O. Oxidative stress: a theoretical model or a biological reality? C R Biol. 2004;327(7):649-62.
- Williams, J.A., Wiener, G., Anderson, P.H., McMurray, C.H.,.
Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood in
various breed crosses of sheep. Research Veterinary Science. 1983;
34: 253–256
- Aebi H. Catalase In: Packer L, editor.Methods in enzymology.
Orlando: Academic Press; 1984. p. 105. 121-6.
- Beutler, E., Duron, O., Kelly, B.M.,. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61: 882-90.
- Paglia D.E., Valentine W.N.,.Studies on the quantitative and
qualitative charaterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70 (19) : 158-169.
- Gutteridge, J.M.,.Lipit peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem. 1995;41(12): 1819–1828.
- Oberley L.W. Representetive of Polypeptid Structure of Bovine
CuZnSOD. Superoxide Dismutase, 1982;1:28
- Younus H. Therapeutic potentials of superoxide dismutase.
International Journal of Health Sciences. 2018;12(3):88-93.
- Darwish, Hossam & Toson, Elshahat & Hatem, A & T El Zanaty,
Ghada & A Abdel, Camelia. oxidative stress-Antioxidant status
in Egyptian Lymphoma patients.. Nature and Science of Sleep.
2012;10:31-37
- Sander çalışma Sander CS, Hamm F, Elsner P, Thiele JJ. Oxidative
stress in malignant melanoma and non-melanoma skin cancer. Br J
Dermatol. 2003 ;148(5):913-22.
- Abou-Seif MA, Rabia A, Nasr M. Antioxidant status, erythrocyte
membrane lipid peroxidation and osmotic fragility in malignant
lymphoma patients. Clin Chem Lab Med. 2000;38(8):737-42.
- Glorieux C, Calderon PB. Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment
approach. Biol Chem. 2017;398(10):1095-1108.
- Jayashree G, Kurup Muraleedhara G, Sudarslal S, Jacob VB. Antioxidant activity of Centella asiatica on lymphoma-bearing mice.
Fitoterapia. 2003 ;74(5):431-4.
- Shen Y, Li D, Tian P, Shen K, Zhu J, Feng M, et al. The catalase C262T gene polymorphism and cancer risk: a systematic review and
meta-analysis. Medicine (Baltimore) 2015 ;94:e679.
- Corso CR, Acco A. Glutathione system in animal model of solid
tumors: From regulation to therapeutic target. Crit Rev Oncol
Hematol. 2018;128:43-57.
- Singh Ghalaut V, Kharb S, Ghalaut PS, Rawal A. Lymphocyte
glutathione levels in acute leukemia. Clin Chim Acta. 1999;285
(1-2):85-9.
- Navarro J, Obrador E, Carretero J, Petschen I, Aviñó J, Perez P,
et al. Changes in glutathione status and the antioxidant system in
blood and in cancer cells associate with tumour growth in vivo. Free
Radic Biol Med. 1999;26(3-4):410-8.
- Jiao Y, Wang Y, Guo S, Wang G. Glutathione peroxidases as oncotargets Oncotarget 2017;8(45):80093-80102.
- Lu YP, Lou YR, Yen P, Newmark HL, Mirochnitchenko OI, Inouye
M, et al.Enhanced skin carcinogenesis in transgenic mice with high
expression of glutathione peroxidase or both glutathione peroxidase and superoxide dismutase.Cancer Res. 1997;57(8):1468-74.
- Walshe J, Serewko-Auret MM, Teakle N, Cameron S, Minto K,
Smith L, et al. Inactivation of glutathione peroxidase activity contributes to UV-induced squamous cell carcinoma formation. Cancer
Res. 2007;67(10):4751-8.
- Björkhem-Bergman L, Jönsson K, Eriksson LC, Olsson JM,
Lehmann S, Paul C,Björnstedt M. Drug-resistant human lung cancer
cells are more sensitive to selenium cytotoxicity. Effects on thioredoxin reductase and glutathione reductase. Biochem Pharmacol.
2002;63(10):1875-84.
- Tisdale MJ, Mahmoud MB. Activities of free radical metabolizing
enzymes in tumours. Br J Cancer 1983 Jun;47(6):809-12.
- Pirincci N, Kaba M, Gecit I, Gunes M, Yuksel MB, Tanik S, et al. Serum prolidase activity, oxidative stress, and antioxidant enzyme
levels in patients with renal cell carcinoma. Toxicol Ind Health.
2016;32:193e9
- Morabito F, Cristani M, Saija A, Stelitano C, Callea V, Tomaino A,
et al. Lipid peroxidation and protein oxidation in patients affected
by Hodgkin's lymphoma. Mediators Inflamm. 2004;13(5-6):381-3.
- Kaya E, Keskin L, Aydogdu I, Kuku I, Bayraktar N, Erkut MA.
Oxidant/antioxidant parameters and their relationship with chemotherapy in Hodgkin's lymphoma. J Int Med Res. 2005;33(6):687-92.
- Güven M, Oztürk B, Sayal A, Ozet A. Lipid peroxidation and
antioxidant system in the blood of patients with Hodgkin's disease.
Clin Biochem. 2000;33(3):209-12.
- Czajkowski R, Czajkowska A, Drewa T, Olszewska D, Zegarski W,
Piech J,et al. Activity of antioxidant enzymes in melanoma patients.
Int J Dermatol. 2013;52(11):1454-6.
- Han J, Colditz GA, Hunter DJ. Manganese superoxide dismutase
polymorphism and risk of skin cancer (United States). Cancer
Causes Control. 2007;18(1):79-89.
- Lu, Yao-Ping, et al. "Enhanced skin carcinogenesis in transgenic
mice with high expression of glutathione peroxidase or both glutathione peroxidase and superoxide dismutase." Cancer Research
1997;57(8): 1468-1474.
- Pence, Barbara C., and Mark F. Naylor. "Effects of single-dose
ultraviolet radiation on skin superoxide dismutase, catalase, and
xanthine oxidase in hairless mice." Journal of investigative dermatology 1990; 95(2): 213-216.
- Iizawa, O., et al. "Long-term follow-up study of changes in lipid
peroxide levels and the activity of superoxide dismutase, catalase
and glutathione peroxidase in mouse skin after acute and chronic
UV irradiation." Archives of dermatological research 1994; 286(1):
47-52.
- Seifirad S, Ghaffari A, Amoli MM. The antioxidants dilemma:
are they potentially immunosuppressants and carcinogens? Front
Physiol. 2014;15:245.