Osman GEDİK, Neslihan TAŞAR, Yaşar KIRAN

Cıva’nın Allium sativum’ un Kök Ucu Hücreleri Üzerindeki Mitotik Etkileri

Bu çalışmada, önemli çevre kirleticilerinden biri olan cıva (HgCl2)’nın sarımsak (Allium sativum) kök ucu hücrelerinin mitoz bölünmeleri üzerine etkileri araştırılmıştır. Denemelerde Hg+2’nın farklı konsantrasyonları (0.015625, 0.03125, 0.0625, 0.125, 0.250, 0.500, 1.000 ve 2.000 mM) kullanılmıştır. Düşük Hg+2 konsantrasyonları ile muamele edilen sarımsakların çimlenmesinde kontrole göre belirgin bir farkın olmadığı, ancak yüksek konsantrasyonlarda çimlenmenin azaldığı ve 0.250 mM üzerindeki örneklerden kök gelişiminin tamamen durduğu gözlenmiştir. Ayrıca uygulanan tüm konsantrasyonlarda, kök büyümesi kontrole göre engellenmiştir. Cıva’ nın konsantrasyon artışına paralel olarak, hücre bölünmesinin azaldığı, kalgın kromozom, multipolar anafaz, anafazda köprü ve granülleşme gibi çeşitli mitotik anormalliklerin arttığı tespit edilmiştir.

Anahtar Kelimeler:

Cıva, Allium sativum, Mitoz, Ağır metal

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In this study, the effects of mercury (HgCl2) one of the significant environmental pollutant, on mitotic divisions of the root tip cells of garlic (Allium sativum) were investigated. Different concentrations (0.015625, 0.03125, 0.0625, 0.125, 0.250, 0.500, 1.000 ve 2.000 mM Hg+2) of mercury were applied. It was observed that there are no significant differences in the germination of seeds that exposed to low mercury concentrations. On the other hand, at higher concentrations of mercury inhibited germination and root growth was stoped above 0.250 mM concentrations. In addition, root growth was inhibited according to the control group at all concentrations. In parallel to the increase of the mercury concentrations cell division was decreased, several mitotic anomalies such as, lagging chromosomes, multipolar anaphases, chromosome bridges andgranulation were increased

Keywords:

Mercury, Allium sativum, Mitosis, Heavy metals,

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