Bazı Mantar Türlerinin Lakkaz Aktivitelerinin Kıyaslanması ve Clytocibe nebularis Türünün Biyosensör Sistemlerine Uygulanabilirliğinin İncelenmesi

Lakkaz, moleküler oksijen kullanarak fenolik bileşikleri yükseltgeyen ve mantarlarda yaygınca bulunan bir enzimdir. Bu çalışmada, Stropharia aeruginosa, Trametes versicolor, Hypholoma fasciculare, Cantharellus cibarius, Clytocibe nebularis ve Amanita muscaria gibi mantar türlerinin lakkaz aktivitelerinin yanı sıra toplam protein miktarlarıının belirlenmesi ve kıyaslanması amaçlanmaktadır. Sonuçlar değerlendirildiğinde, en yüksek lakkaz aktivitesi değerinin Trametes versicolor türüne, en düşük aktivite değerinin de Amanita muscaria türüne ait olduğu gözlenmiştir. Ayrıca, Clytocibe nebularis ve Amanita muscaria türlerinin lakkaz aktivitesi ilk kez bu çalışmada rapor edilmiştir. Çalışmanın bir diğer hedefi de aktivitesi değeri görece yüksek olan ve daha önce biyosensör sistemlerinde kullanılmamış bir mantar türünün, doku homojenatı temelli bir biyosensör yapımında kullanılmasıdır. Bu bağlamda, Clytocibe nebularis dokusu kullanılarak geliştirilen biyosensör ile 100 – 1000 μM aralığındaki artan katekol konsantrasyonları için doğrusal bir amperometrik yanıt elde edilmiştir.

Comparison of Laccase Activities of Some Fungi and Investigation of Applicability of Clytocibe nebularis species to Biosensor Systems

Laccase is an enzyme commonly found in fungi that oxidizes phenolic compounds using molecular oxygen. This study aims to determine and compare the total protein amount as well as the laccase activities of fungal species such as Stropharia aeruginosa, Trametes versicolor, Hypholoma fasciculare, Cantharellus cibarius, Clytocibe nebularis and Amanita muscaria. When the results were evaluated, it was observed that the highest laccase activity value belongs to Trametes versicolor species and the lowest activity value belongs to Amanita muscaria species. Moreover, laccase activity of Clytocibe nebularis and Amanita muscaria species was reported for the first time in this study. Another goal of the study was to use a fungal species with relatively high activity value, which has not been used in biosensor systems before, to construct a tissue homogenate-based biosensor. Therefore, a linear amperometric response was obtained for increasing catechol concentrations in the range of 100 – 1000 μM with the biosensor developed using the Clytocibe nebularis.

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