Ömer ERTÜRK, Derya GÜREL, Murat KORKMAZ

Insecticidal effects of some Streptomycetes strains isolated from soil samples against the larvae and adults of the Leptinotarsa decemlineata (Say, 1824) (Coleoptera, Chrysomelidae)

ÖZ Bu çalışmada Türkiyenin farklı toprak ve coğrafik alanlarından izole edilen otuz yedi Streptomycetes suşunun üç farklı solüsyonu (A, B, C), Leptinotarsa decemlineatanın (Say.) (Coleoptera: Chrysomelidae) 1-4 instar larva ve erginlerine karşı böcek öldürücü etkilerinin incelenmesi için kullanılmıştır. Otuz yedi suşun solüsyonları (A, B, C), L decemlineatanın larva (1-4 instar) ve erginlerine yiyecek (patates yaprağı) vasıtasıyla verilmiştir. Sekiz günü takip eden bioassay çalışmasında larvalar ölmeden önce farklı belirtiler göstermiş; genellikle hareketlerde yavaşlama sergilemiş, iştah ve renk kaybetmişlerdir. Bioassay çalışması boyunca erginlerde de ölmeden önce larvalar gibi hareketlerde yavaşlama, iştah ve renk kaybı gibi belirtiler gözlenmiştir. Bu çalışmaya göre; L. decemlineata larvalarında Streptomycetes suşlarının önemli derecede öldürücü etkisinin olduğu gözlemlenmiştir. Larvalarda, Streptomycetes suşlarından M1483 ve M1282nin (C) solüsyonu %93.1; Streptomycetes suşlarından M3024ün (B) solüsyonu %96.6 ve Streptomycetes suşlarından M4010nun (A) solüsyonu %98.3 ölüm oranı göstermiştir. Öte yandan L. decemlineata erginlerinde Streptomycetes suşlarının önemli derecede öldürücü etkisinin olmadığı gözlemlenmiştir. Sadece Streptomycetes suşlarından (B) solüsyonu M7002de %56.4 ölüm oranı göstermiştir. Bu çalışmanın sonuçları bazı Streptomycetes suşlarının L. decemlineatanın biyokontrolünde kullanılma potansiyeli olduğunu göstermiştir.

Topraktan izole edilen bazı Streptomycetes türlerinin Leptinotarsa decemlineata (Say, 1824) (Coleoptera, Chrysomelidae) ergin ve larvalarına karşı insektisidal etkileri

Thirty-seven Streptomycetes strains isolated from different soils and geographical areas in Turkey were used in this study to investigate the insecticidal effects of their three different solutions (A, B, C) on 1-4 th instar larvae and adults of the Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae). The solutions (A, B, C) of 37 strains were given to larvae (1-4 instar) and adults of L. decemlineata through the food plant (potato leaves). Following eight days of bioassay, larvae showed different signs before death; generally the larvae displayed sluggishness, loss of appetite and depth of color. As in larvae, during bioassays, adults showed different signs before death; generally the adults displayed sluggishness, loss of appetite and depth of color. According to this study; considerable lethal effect of some Streptomycetes sp. were observed on the larvae (1-4 instar) of L. decemlineata, 93.1% larval mortalities caused by (C) solution of Streptomycetes strains M1483 and M1282; 96.6% larval mortalities caused by (B) solution of Streptomycetes strain M3024 and 98.3% larval mortalities caused by (A) solution of Streptomycetes strain M4010. On the other side, no significant mortality effect of Streptomycetes strains was observed on adults of L. decemlineata. Only Streptomycetes strains (B) solution showed 56.4% mortality at M7002. Results of this study indicate that some Streptomycetes strain solutions have a potential use in the bio-control of L. decemlineata.

PDF

___

Abbott W. S. 1925. A method of computing the effectiveness of an insecticide. J. Economic Entomology, 18, 265-267.
Ando K., Oishi H., Hirano S., Okutomi T., Suzuki K., Okazaki H., Sawada M. and Sagawa T. 1971. Tetraranactin, a new miticidal antibiotic. I. Isolation, characterization and properties of tetranactin. J. Antibiotics, 24, 347-352.
Bream A. S., Ghazal S. A., Abd el- Aziz Z. K. and Ibrahim S. Y. 2001. Insecticidal activity of selected actinomycete strains against the Egyptian cotton leaf worm Spodoptera littoralis (Lepidoptera: Noctuidae). Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet., 66(2a), 503-12.
Box S. J., Cole M. and Yeoman G. H. 1973. Prasinons A and B: Potent insecticides from Streptomyces prasinus. Appl. Microbiol., 26, 699-704.
Burg R. W., Miller B. M., Baker E. E., Birnbaum J., Currie S. A., Hartman R., Kong Y. L., Monaghan R. L., Olson G., Putter I., Tunac J. B., Wallick H., Stapley E. O., Oiwa R. and Omura S. 1979. Avermectins, new family of potent anthelmintic agents: Producing organism and fermentation. Antimicrob. Agents Chemother., 15 (3), 361 367.
Carter G. T., Schlingmann G., Kenion G. B., Milne L., Alluri M. R., Korshalla J. D., Williams D. R., Pinho F. and Borders D. B. 1994. Martinomycin, a new polyether antibiotic produced by Streptomyces salvialis. II. Isolation and structure determination. J. Antibiotics, 47, 1549-1553.
Dähn U., Hagenmaier H., Höhne H., König W. A., Wolf G. and Zähner H. 1976. Stoffwechselprodukte von mikroorganismen. 154. Mitteilung. Nikkomycin, ein neuer hemmstoff der chitinsynthese bei pilzen. Arch Microbiol.,107 (2), 143160.
Dhanasekaran D., Sakthi V., Thajuddin N. and Panneerselvam A. 2010. Preliminary evaluation of Anopheles mosquito larvicidal efficacy of mangrove actinobacteria. International Journal of Applied Biology and Pharmaceutical Technology,1 (2), 374- 381.
Dindo M. L. 1993. The potential of plant compounds in insect control. La difesadelle- piante. 16 (1), 23-44.
Dulmage H. T. 1981. Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control, p.193-280.
Burges, H. D. (ed). In: Microbial Control of Pest and Plant Diseases, Academic Press, London. El-Tarabily K. A., Soliman M. H., Nassar A. H., Al-Hassani H. A., Sivasithamparam K., McKenna F. and Hardy G. E. 2000. Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol., 49, 57383.
ElTarabily K. A. 2003. An endophytic chitinaseproducing isolate of Actinoplanes missouriensis, with potential for biological control of root rot of lupin caused by Plectosporium tabacinum. Australian J. Botany, 51, 25766.
Fabre B., Armau E., Etience G., Legendre F. and Tiraby G. 1988. A simple screening method for insecticidal substances from Actinomycetes. J. of Antibiotics, 41 (2), 212-219.
Gürel D. 2006. Rizosferden izole edilen antimikrobiyal aktiviteli Streptomyces' lerin nümerik taksonomisi. Yük. Lis. Tezi. Ondokuz Mayıs Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Samsun, Türkiye. Heisey R. M., Huang J., Mishra S. K., Keller J. E., Miller J. R., Putnam A. R. and D'Silva T. D. J. 1988. Production of valinomycin, an insecticidal antibiotic, by Streptomyces griseus var. flexipertum var. nov. J. Agric. Food Chem., 36 (6), 1283-1286.
Huamei L., Sheng Q., Yongxia W., Wenjun L. and Jie Z. 2008. Insecticidal action of Quinomycin A from Streptomyces sp. KN-0647, isolated from a forest soil. World J. Microbiol. Biotechnol., 24, 2243-2248.
Jizba J., Samoukina G. V., Ivanova-Kovacheva T., Kovacheva T. and Kadybin N. V. 1992. Insecticidal activity of pyrrolizine derivatives isolated fromStreptomyces griseus.Folia Microbiol., 37, 461462.
Kroiss J., Kaltenpoth M., Schneider B., Schwinger M., Hertweck C., Maddula R., Strohm E. and Svato A. 2010. Symbiotic streptomycetes provide antimicrobial combination prophylaxis for wasp offspring. Nature Chemical Biology, 6, 261-263 Doi: 10.1038NCHEMBIO.331.
Mishra S. K., Keller J. E., Miller J. R., Heisey R. M., Nair M. G. and Putnam A. R. 1987. Insecticidal and nematicidal properties of microbial metabolites. J. Ind. Microbiol., 2: 5, 267-276.
Nair M.G., Chandra A. and Thorogood D. L. 1993. Griseulin, a new nitro-containing bioactive metabolite produced by Streptomyces spp. J. Antibiotic, 46, 1762-1763.
Norgard R. B. 1976. Integration economics and pests management. In: Lawrence J. A.; Smith R. E. (Eds) Integrated Pest Management. 63-76.
Plenum Press, New York. Purcell J. P., Greenplate J. T., Jennings M. G., Ryerse J. S., Pershing J. C., Sims S. R., Prinsen M. J., Corbin D. R., Tran M., Sammons R. D. and Stonard R. J. 1993. Cholesterol oxidase: a potent insecticidal protein active against boll weevil larvae. Biochem. Biophys. Res. Commun., 196, 14061413.
Shi Y. F. 2000. Advances of insecticidical microorganisms. Plant Protection, 26, 3234.
Shopotova L. P., Yu Shenin D. and Baikova I. V. 1993. Novel naturel insecticide produced by Streptomyces aurantiacus. Russian Journal of Applied Chemistry C/C of Zhurnal Prikladnoi Khimii., 66, 5, 913.
Reguera G. and Leschine S. B. 2001. Chitin degradation by cellulolytic anaerobes and facultative aerobes from soils and sediments. FEMS Microbiol. Lett., 204: 36774.
Takahashi N., Suzuki A., Kimura Y., Miyamoto S., Tamura S., Mitsui T. and Fukami J. 1968. Isolation, structure, and physiological activities of piericidin B, natural insecticide produced by a Streptomyces. Agr. Biol. Chem., 32, 1115-1122.
Takiguchi Y., Mishima H., Okuda M., Terao M., Aoki A. and Fukuda R. 1980. Milbemycins, a new family of macrolide antibiotics: Fermentation, isolation and physico-chemical properties. J. Antibiotics, 33, 1120-1127.
Thiery I. and Frachon E. 1997. Bacteria: identification, isolation, culture and preservation of entomopathogenic bacteria. In: Lawrence A Lacey, Manual of Techniques in Insect Pathology, Cap. III-1, Biological Techniques Series, Academic Press, London, p. 55-75.
Tripathi A. K., Khanuja S. P. S. and Kumar S. 2002. Chitin synthesis inhibitors as insect pest control agents. J. Medicinal and Aromatic Plant Sci., 24, 10422.
Urushibata I., Isogai A., Matsumoto S. and Suzuki A. 1993. Respirantin, a novel insecticidal cyclodepsipeptide from Streptomyces. J. Antibiotics, 46, 701-703.
Vijayan V. and Balaraman K. 1991. Metabolites of fungi and actinomycetes active against mosquito larvae. Indian-J. Med. Res. Section-A,-Infectious-Diseases. 93: March, 115-117.
Yamaguchi I. 1992. Natural products as agrochemicals and leads. Extended Summary SCI Pesticides Group Symposium. Novel approaches in agrochemical research III. PesticSci., 35, 391-392.
Zhang D., Muraleedharan G., Murry N. M. and Zhang Z. 1997. Insecticidal activity of Indanomycin. J. Antibiot., 50 (7), 617-620.
Zhang H., Huang X., Fukamizo T., Muthukrishnan S. and Kramer K. J. 2002. Sitedirected mutagenesis and functional analysis of an active site tryptophan of insect chitinase. Insect Biochem. Molec., 32, 1477 88.
Xie M. J. 1998. The perspective of the studies on microbial insecticides. Journal of Liaoning Normal University (Natural Science) 21, 326329.