Growth and antioxidant responses of potato (Solanum tuberosum, cv Agria) shoots cultured in vitro under different fusaric acid and boron concentrations1

Farklı fusarik asit ve bor konsantrasyonları altında in vitro da kültüre alınan patates (Solanum tuberosum cv. Agria) sürgünlerinin gelişimi ve antioksidan tepkileriBu çalışmada, in vitro da kültüre alınan patates çeşidine (Solanum tuberosum cv Agria) farklı konsantrasyonlarda uygulanan bor (B), fusarik asit (FA), B X FA interaksiyonuna karşı prolin ve peroksidaz enzim miktarı araştırılmıştır. Sürgün uçları ayrı ayrı B (0, 1, 3, 5, 10 mM), FA (0, 0.1, 0.3, 0.5 mM), 0.1 mM FA X 3 mM B içeren MS ortamı üzerinde 4 hafta süreyle kültüre alınmıştır. Yapılan değerlendirmeler sonucunda 0-1 mM B, 0-0.1 mM FA içeren ortam üzerinde kültüre alınan sürgün uçlarında, sürgün uzunluğu, kök uzunluğu ve bitki yaş ağırlığı açısından gelişmenin diğer uygulamalara göre daha yüksek olduğu belirlenmiştir. 3-5 mM B, 0.3-0.5 mM FA içeren ortam üzerinde kültüre alınan sürgün uçlarında sararmalar, kök uzunluğunda azalmalar tespit edilmiştir. 0.1 mM FA X 1 mM B ve 0.1 mM FA X 3 mM B, içeren ortam üzerinde gelişen sürgünlerde kök gelişimi gözlenmemiş, fakat 0.1 mM FA X 1 mM B ortamında gelişen bitkilerde bitki boyu uzunluğu 0.1 mM FA X 3 mM B' da ki bitki boy uzunluğundan daha fazla olmuştur. Farklı konsantrasyonlarda FA, B ve FA X B uygulamasının prolin miktarını arttırdığı tespit edilmiştir. 0.1, 0.3 ve 0.5 mM FA uygulamasında protein miktarının kontrol ve diğer uygulamalardan daha düşük seviyede olduğu belirlenmiştir. Bunun yanı sıra peroksidaz enzim miktarı (POD) 0.3 ve 0.5 mM FA uygulamasında, kontrol ve diğer uygulamalara kıyasla daha düşük seviyede olmuştur

Farklı fusarik asit ve bor konsantrasyonları altında in vitro da kültüre alınan patates (Solanum tuberosum cv. Agria) sürgünlerinin gelişimi ve antioksidan tepkileri

In this study, the effect of different concentrations of boron (B), fusaric acid (FA), and interaction of B X FA was investigated on the amount of proline and peroxidase enzyme of potato plants (Solanum tuberosum cv. Agria) cultured in vitro. Shoot tips of Agria were cultured into MS medium containing separately B (0, 1, 3, 5, 10 mM), FA (0, 0.1, 0.3, 0.5 mM), different FA X B (0.1 mM FA X 0.1 mM B, 0.1 mM FA X 3 mM B) for a 4 weeks. As a result, shoot length, root length, weight of plant by the 0.1 mM B and 0, 0.1 mM FA were higher than other applications. The application of 3, 5 mM B and 0.3, 0.5 mM FA caused yellowing, root length reductions. It was not observed the root growth the application of different 0.1 mM FA X 1 mM B and 0.1 mM FA X 3 mM B, but the plant length application of 0.1 mM FA X 1 mM B was higher than 0.1 mM FA X 3 mM B. It was determined an increase in proline for amount the application of different FA, B and FA X B. Also, the amount of protein of different concentrations of 0.1, 0.3 and 0.5 mM FA was determined lower than other application and control plants. However, the amount of the enzyme peroxidase (POD) was found lower in the application of 0.3 and 0.5 mM FA than other application and control plant

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