Ebru KOCADAĞ KOCAZORBAZ, Ayşe Yağmur AZBAZDAR, Merve ALİAKAR ÖZTÜRK, Esra MENFAATLİ

Cyclamen Mirabile Soğanından Lektin Saflaştırılması ve Karakterizasyonu

Bu çalışmada, Türkiye'nin endemik bir türü olan Cyclamen mirabile soğanından tek adımda lektin saflaştırıldı. Yöntem olarak, amonyum sülfat ve ardından kalsiyum aljinat presipitasyonu kullanıldı. Saflaştırılan lektin SDS PAGE ile analiz edildiğinde 13.5 ve 14.8 kDa molekül kütlelerine sahip iki protein bandı gözlendi. Hemaglütinasyon inhibisyon yöntemi ile C. mirabile soğanından elde edilen lektinin mannoz spesifik lektin olduğu belirlendi. C. mirabile lektini geniş bir pH aralığında ve 4-40°C arasında hemaglütinasyon aktivitesini korudu. MgCl2 ve HgCl2 metallerinin lektinin hemaglütinasyon aktivitesini inhibe ettiği görüldü.

Purification and Characterization of a Lectin From Bulbs of Cyclamen Mirabile

Lectin from the bulbs of Cyclamen mirabile which is an endemic species of Turkey was successfully isolated by affinity precipitation with alginate in one step. The purified protein produced two bands showing a dimeric structure in SDS-PAGE (13.5 and 14.8 kDa). C. mirabile lectin showed activity and stability in a broad pH scale and kept its haemagglutination activity in the temperature range of 4-40°C. MgCl2 and HgCl2 inhibited the haemagglutination activity of the lectin. In this study, a practical and efficient purification procedure was carried out for C. mirabile lectin by using affinity precipitation with alginate.

Keywords:

Lectin, Protein Purification, Cyclamen Mirabile,

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