Tuba YALÇIN, Sercan KAYA, Nalan KAYA TEKTEMUR, İ. Enver OZAN

Testis Dokusunun Histopatolojik Değerlendirilmesinde Kullanılan Yöntemler

Kantitatif ölçümler, çeşitli süreçlerin çıktıları olan sayısal verilerdir. Toplanan verilerin istatiksel analizleri yapılmaktadır. Bu istatiksel verilerden elde edilen sonuçlar daha güvenilir olarak kabul edilmektedir. Bu nedenle günümüzde ölçüm işlemleri araştırma bilimlerinin temelini oluşturmaktadır. Histoloji, hücrelerin ve dokuların mikroskopik yapısını inceleyen bilim dalıdır. Testis, histopatoloji, kantitatif, seminifer tübül kelimelerini MEDLINE, SCIENCE DIRECT, Web of Science, Springer Link, Ovid, ve PubMed arama motorları kullanılarak literature taramaları yapıldı.Hücre ve doku örneklerinden elektron ve ışık mikroskobu kullanılarak morfometrik ölçümler yapılabilmektedir. Bu kalitatif yöntemlerle elde edilen bulgular doku morfolojisindeki değişikliklerin yorumlanmasını sağlamaktadır. Bu değerlendirmeler araştırmacıların doku örneklerini tanımasını ve morfolojisindeki fizyolojik varyasyonların karşılaştırılmasını sağlamaktadır. Histolojide yapılan laboratuvar çalışmalarında rutin boyamalarda elde edilen sonuçlar kalitatif olduğundan, sonuçların yorumlanması aşamasında görece farklılıklar ortaya çıkabilir. Bu riski ortadan kaldırmak için çeşitli kantitatif ölçüm yöntemleri kullanılmaktadır. Günümüzde, doğru değerlendirme yapabilmek için testis, karaciğer, akciğer, böbrek gibi organlarda kullanılan histometrik ve stereolojik ölçüm yöntemleri önem arz etmektedir. Bu işlemler sonucu elde edilen kantitatif veriler, istatistiksel analizler için yeterlidir. Ayrıca istatiksel analizler yapılırken elde edilen kalitatif ya da semikantitatif verilerin tekrarlanmasında belirli bir standardizasyonun yakalamasında önem arz etmektedir. Bu çalışmanın amacı testis dokusunun histopatolojik değerlendirilmesinde kullanılan yöntemleri özetlemektir.

Anahtar Kelimeler:

Testis, Histopatoloji, Kantitatif, Seminifer Tübül

The Methods Used in Histopathological Evaluation of Testis Tissues

Introduction: The quantitative measurements are numerical data as the outputs of various processes. Statistical analysis is employed to the collected data. Results obtained from the statistical data are considered as more reliable. Therefore, measurement processes are the basis of research sciences today. Histology is a discipline that examines the microscopic structure of cells and tissues. Morphometric measurements can be performed on cell and tissue samples through electron and light microscope.Materials and Methods: MEDLINE, SCIENCE DIRECT and Web of Science, Springer Link, Ovid, in the PubMed search engine were screened with the words “Testis, histopathology, quantitative, seminiferous tubule” between to find the studies.Results: The findings obtained through these qualitative methods provide interpretation of the changes in tissue morphology. These assessments allow researchers to identify the tissue samples and to compare the physiological variations in its morphology. In histology, the results obtained from routine dyeing of laboratory studies are qualitative, therefore, relative differences may emerge in the interpretation of the results. In order to eliminate this risk, various quantitative measurement methods are implemented.Discussion and Conclusion: Today, in order to employ accurate evaluations, the histometric and stereological measurement methods that are used in organs such as testicles, liver, lung, and kidney gain importance. The quantitative data obtained from these transactions is sufficient for statistical analysis. It is also important to reach a certain standardization level in the repetition of qualitative or semi-quantitative data obtained during the statistical analyses. The aim of this study is to summarize the methods used in histopathological evaluation of testicular tissues.

Keywords:

Testis, Histopathology, Quantitative, Seminiferous Tubule,

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