Cerrahi Menopoz ve Kronik Serebral Hipoperfüzyon Oluşturulmuş Sıçanlarda Spironolaktonun Prefrontal Korteks ve Göz Dokularında Glikojen ve Oksidatif Stres Parametreleri Üzerine Etkisi

Amaç: Kronik serebral hipoperfüzyon (KSH), ilerleyici hipokampal hasar ve bilişsel bozukluklarla birlikte vasküler demansa yol açar. KSH retinadaki kan akımında da azalmaya neden olmaktadır. Bu çalışmanın amacı KSH oluşturulmuş overektomize sıçanlarda spironolaktonun göz ve prefrontal korteks (PFC) dokularındaki oksidatif stres ve glikojen seviyelerine etkilerini araştırmaktır. Gereç ve Yöntemler: Wistar Albino cinsi 32 adet dişi sıçan, her grupta 8 adet olacak şekilde rastgele 4 gruba ayrıldı: 1)Kontrol, 2)KSH, 3)KSH+Spironolakton 25mg/kg 4)KSH+Spironolakton 50mg/kg. Sıçanlara deneyin başlangıcında bilateral ovariektomi (OVX) uygulandı, OVX’ten 5 hafta sonra ortak bilateral ortak karotid arter oklüzyonu (2VO) ile KSH modeli oluşturuldu. Spironolakton 2VO’dan üç gün önce ve 2VO’dan sonra 3 hafta boyunca günde 1 kez oral gavaj yoluyla uygulandı. Deney sonunda doku glikojen, indirgenmiş glutatyon (GSH), malondialdehid (MDA) ve AA düzeyleri ölçüldü. İstatistiksel analiz ANOVA ve Bonferroni testleri kullanılarak yapıldı. Bulgular: KSH, PFC’de oksidatif stres belirteçlerinde artışa ve glikojen düzeyinde bir azalmaya neden oldu. Benzer şekilde KSH göz dokusunda hem glikojen hem de AA düzeyinde azalmaya neden oldu. Spironolaktonun 25 mg/kg dozu ile tedavi edilen grupta KSH grubuna kıyasla göz AA ve PFC glikojen düzeyleri azalırken PFC’deki MDA düzeyi artmış olarak bulundu (p

Effect of Spironolactone on Glycogen and Oxidative Stress Parameters in Prefrontal Cortex and Eye Tissues in Rats in Which Surgical Menopause and Chronic Cerebral Hypoperfusion Performed

Aim: Chronic cerebral hypoperfusion (CCH) leads to vascular dementia with progressive hippocampal damage and cognitive impairments. CCH also causes a decrease in blood flow in the retina.The aim of this study was to investigate the effects of spironolactone on oxidative stress and glycogen levels in the eye and prefrontal cortex (PFC) tissues in ovariectomized rats with CCH. Material and Methods: 32 female Wistar Albino rats were randomly divided into 4 groups, 8 in each group: 1)Control, 2)KSH, 3) KSH+Spironolactone 25mg/kg 4)KSH+Spironolactone 50mg/kg. The rats underwent bilateral ovariectomy (OVX) at the start of the experiment, a CCH model was created with permanent occlusion of the common carotid arteries (2VO) 5 weeks after OVX. Spironolactone was administered by oral gavage three days before 2VO and once daily for 3 weeks after 2VO. At the end of the experiment, tissue glycogen, reduced glutathione (GSH), malondialdehyde (MDA) and ascorbic acid (AA) levels were measured. Statistical analysis was performed using ANOVA and Bonferroni tests. Results: CCH caused an increase in oxidative stress markers and a decrease in glycogen level in PFC. Similarly, CCH caused a decrease in both glycogen and AA levels in the eye tissue. In the group treated with 25mg/kg spironolactone, the AA and PFC glycogen levels of the eyes decreased, while the MDA levels in the PFC were increased compared to the CCH group(p

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