Bilgin KAYGISIZ, Özden KUTLUAY, Ziya KAYGISIZ

The Effect of Chemerin on Cardiac Parameters and Gene Expressions in Isolated Perfused Rat Heart

Background: Chemerin is a novel chemoattractant adipokine expressed in cardiovascular system, and its receptor has been detected in the epicardial adipose tissue.Aims: To determine the effects of chemerin on the cardiac parameters and gene expressions in the isolated perfused rat heart.Study Design: Animal experiment.Methods: The hearts were retrogradely perfused with Langendorff technique to measure the cardiac parameters. The experimental groups were acutely treated with 10, 100, and 1000 nM doses of chemerin. Another group was given 10 μM L-nitric oxide synthase inhibitor for 5 min before 1000 nM chemerin administration. The real-time polymerase chain reaction was performed for detecting the expression of target genes.Results: All doses of chemerin significantly decreased the left ventricular developed pressure (max 35.33 ∆%, p

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Yamawaki H. Vascular effects of novel adipocytokines: focus on vascular contractility and inflammatory responses. Biol Pharm Bull 2011;34:307-10.
Conde J, Scotece M, Gomez F, Lopez V, Gomez-Reino JJ, Lago F, et al. Adipokines: Biofactors from white adipose tissue. A complex hub among inflammation, metabolism, and immunity. Biofactors 2011;37:413-20.
Ferland DJ, Watts SW. Chemerin: A comprehensive review elucidating the need for cardiovascular research. Pharmacol Res 2015;99:351-61.
Rodríguez-Penas D, Feijóo-Bandín S, García-Rúa V, Mosquera-Leal A, Durán D, Varela A, et al. The adipokine chemerin induces apoptosis in cardiomyocytes. Cell Physiol Biochem 2015;37:176-92.
Zhang O, Ji Q, Yingzhong L, Wang Z, Huang Y, Lu W, et al. Circulating chemerin levels elevated in dilated cardiomyopathy patients with overt heart failure. Clin Chim Acta 2015;448:27-33.
Bozaoglu K, Bolton K, McMillan J, Zimmet P, Jowett J, Collier G, et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology 2007;148:4687-94.
Chakaroun R, Raschpichler M, Klöting N, Oberbach A, Flehmig G, Kern M, et al. Effects of weight loss and exercise on chemerin serum concentrations and adipose tissue expression in human obesity. Metabolism 2012;61:706-14. Mattern A, Zellmann T, Beck-Sickinger AG. Processing, signaling and physiological function of chemerin. IUBMB Life 2014;66:19-26.
Kostopoulos CG, Spiroglou SG, Varakis JN, Apostolakis E, Papadaki HH. Chemerin and CMKLR1 expression in human arteries and periadventitial fat: a posssible role for local chemerin in atherosclerosis? Cardiovasc Disord 2014;14:56.
Ferland DJ, Darios ES, Neubig RR, Sjogren B, Truong N, Torres R, et al. Chemerin-induced arterial contraction is Gi-and calcium-dependent. Vasc Pharmacol 2017;88:30-41.
Kennedy AJ, Yang P, Read C, Kuc RE, Yang L, Taylor EJ, et al. Chemerin elicits potent constrictor actions via chemokine-like receptor 1 (CMKLR1), not G-protein-coupled receptor 1 (GPR1), in human and rat vasculature. J Am Heart Assoc 2016:5.
He MX, Downey HF. Downregulation of ventricular contractile function during early ischemia is flow but not pressure dependent. Am J Physiol 1998;275:520-3.
Maruyama S, Shibata R, Kikuchi R, Izumiya Y, Rokutanda T, Araki S, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism. J Biol Chem 2012;287:408-17.
Peng T, Lu X, Lei M, Feng Q. Endothelial nitric-oxide synthase enhances lipopolysaccharide estimulated tumor necrosis factor alpha expression via Camp-mediated p38 MAPK pathway in cardiomyocytes. J Biol Chem 2003;278:8099-105.
Massion PB, Feron O, Dessy C, Balligand JL. Nitric oxide and cardiac function: ten years after, and continuing. Circ Res 2003;93:388-98.
Ozcan G, Şahin EA, Demirel Yılmaz E. Effect of Nitric Oxide on Cardiac Functions: Turkey Clinics J Cardiovasc Sci 2016;28:99-117.
Seddon M, Shah AM, Casadei B. Cardiomyoctes as effectors of nitric oxide signalling. Cardiovasc Res 2007;75:315-26.
Van de Sandt AM, Windler R, Gödecke A, Ohlig J, Zander S, Reinartz M, et al. Endothelial NOS (NOS3) impairs myocardial function in developing sepsis. Basic Res Cardiol 2013;108:330.
Lu Z, Ballou LM, Jiang YP, Cohen IS, Lin RZ. Restoration of defective L-type Ca2+ current in cardiac myocytes of type 2 diabetic db/db mice by Akt and PKC-Ι. J Cardiovasc Pharmacol 2011;58:439-45.
Yano N, Tseng A, Zhao TC, Robbins J, Padbury JF, Tseng YT. Temporally controlled overexpression of cardiac-specific PI3Kalpha induces enhanced myocardial contractility a new transgenic model. Am J Physiol Heart Circ Physiol 2008;295:1690-4.
Ghigo A, Morello F, Perino A, Damilano F, Hirsch E. Specific PI3K Isoform Modulation in Heart Failure: Lessons from Transgenic Mice. Curr Heart Fail Rep 2011;8:168-75.
Kerfant BG, Gidrewicz D, Sun H, Oudit GY, Penninger JM, Backx PH. Cardiac Sarcoplasmic Reticulum Calcium Release and Load Are Enhanced by Subcellular cAMP Elevations in PI3K gamma-deficient mice. Circ Res 2005;96:1079-86.
Crackower MA, Oudit GY, Kozieradzki I, Sarao R, Sun H, Sasaki T, et al. Regulation of myocardial contractility and cell size by distinct PI3K-PTEN signaling pathways. Cell 2002;110:737-49.
Ghigo A, Morello F, Perino A, Damilano F, Hirsch E. Specific PI3K Isoform Modulation in Heart Failure: Lessons from Transgenic Mice. Curr Heart Fail Rep 2011;8:168-75.
Kerfant BG, Rose RA, Sun H, Back PH. Phosphoinositide 3-kinase c Regulates Cardiac Contractility by Locally Controllig Cyclic Adenosine Monophosphate Levels. Trends Cardiovasc Med 2006;16:250-6.
George MS, Pitt GS. The real estate of cardiac signaling: location, location, location. Proc Natl Acad Sci U S A 2006;103:7535-6.
Lobato NS, Neves KB, Filgueira FP, Fortes ZB, Carvalho MH, Webb RC, et al. The adipokine chemerin augments vascular reactivity to contractile stimuli via activation of the MEK-ERK1/2 pathway. Life Sci 2012;15:600-6.
Watts S, Dorrance AM, Penfold ME, Rourke JL, Sinal CJ, Seitz B, et al. Chemerin connects fat to arterial contraction. Arterioscler Thromb Vasc Biol 2013;33:1320-28.
Xia JS, Li Z, Dong JW, Tu H, Zeng, FD. Dauricine-induced changes in monophasic action potentials and effective refractory period of rabbit left ventricle in situ. Acta Pharmacol Sin 2002;23:371-5.
Wang Y, Chen MS, Liu HC, Xiao JH, Wang JL. The relationship between frequency dependence of action potential duration and the expression of TRPC3 in rabbit ventricular myocardium. Cell Physiol Biochem 2014;33:646-56.