Effects on Bacterial Translocation of High-Fat Enteral Nutrition in Bile Duct Ligated Rats

Objective: Bacterial Translocation (BT) from the gastrointestinal system is at the center of current sepsis theories. In patients with obstructive jaundice, the absence of intraluminal bile flow causes some alterations and mucosal damage in the gut. In the present study, it was aimed to investigate the effects on BT of high-fat enteral nutrition in bile duct ligated rats. Material and Methods: In this study, a total of 28 healthy Spraque-Dawley rats, weighing 230-300 gr, were grouped into four as sham group, control group, high-fat enteral nutrition group and low-fat enteral nutrition group. The rats in all the groups were sacrificed on the seventh postoperative day The values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total and direct bilirubin were measured for biochemical evaluation. Also, samples were taken from the blood, lung, liver, spleen and mesenteric lymph nodes for microbiological evaluation. The results were calculated as CFU/gr and evaluated statistically. Results: In all bile duct ligated rats, all findings of obstructive jaundice were observed clinically (in postoperatively third day) and in the laboratory. It was determined that the lymphatic system is an essential pathway for BT, as reported by similar studies. However, it was observed in this study that the high-fat enteral nutrition may be not severely effective in reducing BT in bile duct ligated rats. The results were supported by statistical analyses. Conclusion: It was observed that high-fat enteral nutrition has no meaningful effects on reducing BT in bile duct ligated rats. Turkish Anahtar Kelimeler: Obstruktif sarılık, yüksek yağlı enteral beslenme, bakteriyal translokasyon Amaç: Gastrointestinal sistemden Bakteriyel Translokasyon (BT) güncel sepsis kuramlarının merkezinde yer almaktadır. Tıkanma sarılığı olan hastalarda, intraluminal safra akışının olmaması bazı değişikliklere ve bağırsak mukozasında hasara neden olur. Çalışmamızda, safra kanalı ligasyonu yapılan sıçanlarda yüksek yağ enteral beslenmenin BT üzerindeki etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada, 230-300 gr ağırlığında 28 adet sağlıklı Spraque-Dawley cinsi sıçan, sham grubu, kontrol grubu, yüksek yağlı enteral beslenme grubu ve düşük yağlı enteral beslenme grubu olarak toplam dört gruba ayrıldı. Tüm gruplarda sıçanlar postoperatif yedinci gün sakrifiye edildi. Biyokimyasal değerlendirmede, aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), alkalen fosfataz (ALP), gama-glutamil transferaz (GGT), total ve direkt bilirubin değerleri ölçüldü. Ayrıca mikrobiyolojik değerlendirme için kan, akciğer, karaciğer, dalak ve mezenterik lenf nodlarından örnekler alındı. Sonuçlar CFU / gr olarak hesaplandı ve istatistiksel olarak değerlendirildi. Bulgular: Safra kanalı ligasyonu yapılan tüm sıçanlarda, tıkanma sarılığının tüm bulguları klinik (postoperatif üçüncü günde) ve laboratuar olarak gözlenmiştir. Benzer çalışmalarda da bildirildiği gibi BT için lenfatik sistemin önemli bir yol olduğu tespit edilmiştir. Bununla birlikte, safra kanalı ligasyonu yapılan sıçanlarda yüksek yağlı enteral beslenmenin BT&#39;yi azaltmada ciddi bir etkisi olmadığı görülmüştür. Sonuçlar istatistiksel analizler ile desteklenmiştir. Sonuç: Yüksek yağlı enteral beslenmenin safra kanalı ligasyonu yapılan sıçanlarda BT&#39;nin azaltılması üzerinde anlamlı bir etkisi olmadığı gözlenmiştir.

Keywords:

Obstructive jaundice high-fat enteral nutrition, bacterial translocation,

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