Onur ARGAN, Kübra ÇIKRIKÇI, Nahit GENCER

Sıvı kardiyak ilaçların karbonik anhidraz I ve II izoenzimleri üzerindeki etkileri

Kardiyovasküler hastalıklar dünya çapında ölümlerin önde gelen nedenidir. Son yıllarda karbonik anhidraz inhibitörleri ile ateroskleroz arasındaki ilişki dikkat çekmektedir. Bu çalışmada, sık kullanılan sıvı kardiyak ilaçların insan karbonik anhidraz (hCA) I ve II üzerine in vitro etkilerini belirlemeyi amaçladık. İlaçların hCA I ve hCA II üzerindeki inhibitör etkileri hidrataz aktivitesi ile belirlendi. Test edilen kardiyak ilaçlarının tamamı hCA I ve II izozimlerini inhibine etmiştir. En güçlü inhibitörün Atropin Sülfat (hCA I: 5,86 mM ve hCA II: 6,59 mM) olduğunu saptadık. Heparin Sodyum ve Dopamin HCl de güçlü inhibitörlerdir.

Anahtar Kelimeler:

Kardiyak ilaçlar, karbonik anhidraz, enzim inhibisyonu

Effects of liquid cardiac drugs on carbonic anhydrase I and II ısozymes

Cardiovascular diseases are the leading cause of death worldwide. In recent years, the relationship between atherosclerosis and carbonic anhydrase inhibitors has attracted attention. In this study, we aimed to determine the in vitro effects of commonly used liquid cardiac drugs on human carbonic anhydrase (hCA) I and II. The inhibitory effects of drugs on hCA I and hCA II were determined by hydratase activity. All tested cardiac drugs inhibited hCA I and II isozymes. We found that the strongest inhibitor was Atropine sulfate (hCA I: 5.86 mM and hCA II: 6.59 mM). Heparin Sodium and Dopamine HCl are also potent inhibitors.

Keywords:

Cardiac drugs, carbonic anhydrase, enzyme inhibition,

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