Bazı sedatif ve anestezik aromatik bitki yağlarının gökkuşağı alabalığı (Oncorhynchus mykiss, L.) kan parametreleri üzerine etkileri

Bu çalışmada, geleneksel tıpta sedatif ve anestezik etkileri olduğu bilinen alıç (Crataegus monogyna), limon otu (Melissa officinalis), haşhaş (Papaver somniferum), biberiye (Rosmarinus officinalis)’ den elde edilen yağların 96 saat ve 25 mg/L konsantrasyonda gökkuşağı alabalıklarının kanında (Oncorhynchus mykiss) meydana getirdiği biyokimyasal ve hematolojik değişimler belirlendi. Alıç, limon otu ve haşhaş yağı uygulanan grupların ALT (alanin aminotransferaz), ALP (alkalen fosfataz), AST (aspartat aminotransferaz), LDH (laktat dehidrogenaz), glikoz, kortizol, toplam protein, üre ve kreatinin seviyelerinde kontrol grubu ile karşılaştırıldığında istatistiksel açıdan önemli artışlar (P<0.05), toplam kolesterol, trigliserid, sodyum, potasyum ve demir seviyelerinde ise önemli azalmalar (P<0.05) tespit edildi. Hematolojik parametrelerde ise alıç ve haşhaş yağı uygulanan balıkların eritrosit, hemoglobin ve hematokrit seviyelerinde kontrole göre anlamlı artışlar (P<0.05), lökosit değerinde ise azalma (P<0.05) belirlendi. Biyokimyasal ve hematolojik sonuçlar, literatürde balık anestezisinde doğal ajan olarak sıkça kullanılan karanfil yağının 25-50 mg/L konsantrasyonu ile yapılan çalışmalarda elde edilen kan parametreleri ile karşılaştırıldığında, alıç, haşhaş ve limon otunun da sedasyon ve anestezi için kullanılabileceğini ortaya koymuştur. Bu yüzden sentetik anestezikler yerine doğal anestezikler olarak 25 mg/L konsantrasyonda 96 saat uygulanan alıç, haşhaş ve limon otu yağının balıkların fizyolojik fonksiyonlarının düzenlenmesinde alternatif bir antioksidan takviyesi olarak kullanılabileceği ve onları tüketen organizmaların sağlığına da olumlu katkılar sağlayabileceği düşünülmektedir.

Effects on the blood parameters of the rainbow trout (Oncorhynchus mykiss, L.) of some sedative and anesthetic aromatic plant oils

In this study, the oil obtained from hawthorn (Crataegus monogyna), lemon balm (Melissa officinalis), opium poppy (Papaver somniferum), rosemary (Rosmarinus officinalis) known to have sedative and anesthetic efficacy in traditional medicine were determined biochemical and hematological changes in blood of rainbow throut (Oncorhynchus mykiss) in 96 h and a concentration of 25 mg/L. There were statistically significant increases in the levels of ALT (alanin aminotransferaz), ALP (alkalen fosfataz), AST (aspartat aminotransferaz), LDH (laktat dehidrogenaz), glucose, cortisol, total protein, urea and creatinine levels in the groups treated with hawthorn, lemon balm and opium poppy when compared with the control group (P<0.05) and total cholesterol, triglyceride, sodium, potassium and iron levels were found significant reductions (P<0.05). In hematological parameters; there were significant increases (P<0.05) in erythrocyte, hemoglobin and hematocrit levels of fish treated with hawthorn and opium poppy oil and decrease in leukocyte count (P<0.05). Biochemical and hematological results have shown that hawthorn, opium poppy and lemon balm oil can also be used for sedation and anesthesia when compared to blood parameters obtained from studies with 25-50 mg/L concentration of clove oil commonly used as a natural agent in fish anesthesia. It is believed that hawthorn, opium poppy and lemon balm oil applied in concentration of 25 mg/L for 96 h may be used as an alternative antioxidant supplement in the regulation of the physiological functions of fish and may provide positive contributions to the health of the consuming organisms.

Keywords:

Crataegus monogyna, Melissa officinalis, Papaver somniferum, Rosmarinus officinalis, Blood, Rainbow trout, sedative effect anesthetic effect,

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