Hasan SUSAR, Murat ÇELEBİ, Çağla ÇELEBİ, Özlem ÇOBAN, Hüseyin ŞEN, İzzet KARAHAN

Veteriner Hekimlikte Kullanılan Gentamicin ve Seftiofur'un Lipozomal Formülasyonlarının Hazırlanması

veteriner kullanım için seftiofur ve gentamisinin lipozomal formülasyonlarını üretmek ve kalite kontrol çalışmalarını yapmak hedefleriyle tasarlanmıştır. Gereç ve Yöntem: Gentamisin ve seftiofur’un lipozomal formülasyonlarının hazırlanması amacıyla Bangham Metodu tercih edildi. Bulgular: Sonuç olarak; seftiofur-1 lipozomunun partikül boyutu 3934.67 nm, polidispersite indeksi 0.471, zeta potansiyeli -10.3 mV'dir; Ceftiofur-2 lipozomunda; parçacık boyutu 4573.00 nm, polidispersite indeksi 0.308, zeta potansiyeli -09.9 mV; Ceftiofur-3 lipozomlarında; parçacık boyutu 479.40 nm, polidispersite indeksi 0.437, zeta potansiyeli -44.8 mV ölçülmüştür. Gentamisin-1 lipozomunda; parçacık boyutu 5185.67 nm, polidispersite indeksi 0.599, zeta potansiyeli -06.5 mV; Gentamisin-2 lipozomunda; parçacık boyutu 4228.00 nm, polidispersite indeksi 0.599, zeta potansiyeli -07.8 mV; Gentamisin-3 lipozomunda; parçacık boyutu 2138.67 nm, polidispersite indeksi 0.565, zeta potansiyeli -06.5 mV ölçülmüştür. Sonuç: Seftiofur ve gentamisinin başarılı bir şekilde lipozomal formülasyonları hazırlandı ve kalite kontrol çalışmaları gerçekleştirildi. Lipozomal formülasyonların etkinliklerinin değerlendirilmesi için ise farmakokinetik/farmakodinamik çalışmaların yapılması gerektiği kanaatine varıldı.

The Preparation of Liposomal Formulations of Gentamicin and Ceftiofur Used in Veterinary Medicine

Objective: The aim of this study is to produce liposomal drugs that are scarcely found in veterinary medicine. Therefore, the current study was designed to produce liposomal formulations of ceftiofur and gentamicin for veterinary use and to perform their quality control studies. Materials and Methods: The Bangham Method was chosen for the preparation of liposomal formulations of gentamicin and ceftiofur. Results: The particle size, polydispersity index and zeta potential were found to be 3934.67 nm, 0.471, -10.3 mV for Ceftiofur-1 coded formulation, 4573.0 nm, 0.308, -9.9 mV for Ceftiofur-2 coded formulation, 479.4 nm, 0.437, -44.8 mV for Ceftiofur-3 coded formulation, respectively. The particle size, polydispersity index and zeta potential were found to be 5185.67 nm, 0.599, -6.5 mV for Gentamicin-1 coded formulation, 4228.0 nm, 0.505, -7.8 mV for Gentamicin-2 coded formulation, 2138.67 nm, 0.565, -6.5 mV for Gentamicin-3 coded formulation, respectively. The encapsulation efficiency of liposomal formulations containing ceftiofur; 82.85%, 95.74%, and 92.06% was found for ceftiofur-1, ceftiofur-2 and ceftiofur 3, respectively. Conclusion: The liposomal formulations of ceftiofur and gentamicin were successfully prepared and their quality control studies were carried out. It was concluded that pharmacokinetic/pharmacodynamic studies should be performed to evaluate the efficacy of liposomal formulations.

Keywords:

Drug, Formulation, Liposome, Veterinary.,

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