İvesi Irkı Koyunlarda Yavru Sayısına Göre Amniyon Sıvısındaki Biyokimyasal Değişikliklerin Belirlenmesi.

Amaç: Sunulan çalışma İvesi ırkı koyunlarda doğum zamanı alınan amniyon sıvısı içerisindeki bazı biyokimyasal ve hormonal belirteçlere bakarak ikiz ve tekiz gebelikler arasındaki metabolik ihtiyaç farkını araştırmak için yapıldı. Gereç ve Yöntem: Çalışmada toplam 30 adet İvesi ırkı koyun kullanıldı. Çalışmanın birinci grubunu (Grup 1, n=15); tek yavru taşıyan koyunlar, çalışmanın ikinci grubunu (Grup 2, n=15) ise; iki yavru taşıyan koyunlar oluşturdu. Her iki çalışma gruplarındaki koyunlardan; doğum zamanı vulva dudakları arasından sarkan amniyon kesesinin bütünlüğünü bozmadan steril enjektör ile 10 ml amniyon sıvısı alındı. Alınan amniyon sıvısından elektrolit (sodyum, potasyum, klor, kalsiyum, fosfor), total protein, glikoz, karaciğer (ALT ve AST), böbrek biyomarkerları (üre ve keratinin) ve kortizol düzeyleri ölçüldü. Elde edilen veriler bağımsız gruplar t test ile analiz edildi. Bulgular: Glikoz ve kortizol düzeyleri ikiz gebe grubunda tekiz gebe grubuna göre anlamlı derecede yüksekti (p

Anahtar Kelimeler:

Amniyon sıvısı, İkiz gebelik, Biyokimyasal değişiklik, Kortizol

Determination of Biochemical Changes in Amniotic Fluid According to the Number of Offspring in Awassi Sheep

Aim: The present study was carried out to investigate the difference in metabolic needs between twin and singleton pregnancies by looking at some biochemical and hormonal markers in the amniotic fluid taken at the time of birth in Awassi sheep. Materials and Methods: A total of 30 Awassi sheep were used in the study. The first group of the study (Group 1, n=15); ewes carrying one offspring included the second group of the study (Group 2, n=15); created sheep carrying two offsprings. From the sheep in both study groups; 10 ml of amniotic fluid was taken with a sterile syringe without disturbing the integrity of the amniotic sac hanging from the lips of the vulva at the time of delivery. Electrolyte (sodium, potassium, chlorine, calcium, phosphorus), total protein, glucose, liver (ALT and AST), kidney biomarkers (urea and keratinin) and cortisol levels were measured from the amniotic fluid. Obtained data were analyzed with independent samples t-test. Results: Glucose and cortisol levels were significantly higher in the twin pregnant group than in the singleton pregnant group (p

Keywords:

Amniotic fluid, Twin pregnancy, Biochemical change, Cortisol,

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