+4 °C’de Krebs-Henseleit Solüsyonunda Soğuk Depolamanın Sıçan Torasik Aortunun Vazoreaktivitesi Üzerindeki Etkileri

Amaç: Bu çalışma, Krebs-Henseleit solüsyonunda +4 °C’de 3, 6 ve 18 saat süreyle saklanan sıçan torasik aortlarında endotel-bağımlı ve -bağımsız gevşeme yanıtlarının bozulmadan korunup korunamayacağını araştırmayı amaçlamıştır. Yöntemler: Deneyler izole organ banyosu modeli ve 10-12 haftalık erkek Wistar sıçanlar kullanılarak gerçekleştirilmiştir. Endotel-bağımlı gevşeme faktörlerinin etkisini araştırmak için, endojen prostanoid sentezini inhibe etmek üzere Krebs-Henseleit çözeltisine siklooksijenaz inhibitörü INDO eklenmiştir. Tek doz PE ile submaksimal kasılma yanıtı elde edilmiş ve ardından endotel-bağımlı gevşeme yanıtlarını indüklemek üzere kümülatif olarak (10-9-10-4 M) ACh uygulanmıştır. Ayrıca, PE ile indüklenen prekontraksiyonu takiben SNP kümülatif (10-9-10-5 M) uygulanarak düz kasa bağlı gevşeme yanıtları elde edilmiştir. İstatistiksel anlamlılık düzeyi p0.05). Ayrıca, kümülatif uygulanan ACh, endotel-bağımlı gevşeme yanıtlarında anlamlı bir değişikliğe neden olmamıştır (p>0.05). Benzer şekilde, SNP 3, 6 veya 18 saatlik saklamadan sonra aort segmentlerinde endotel-bağımsız gevşeme yanıtlarını modüle etmemiştir (p>0.05). Sonuç: Bu çalışmada, Krebs-Henseleit solüsyonunda +4 °C’de 3, 6 veya 18 saatlik saklama sürelerinden sonra sıçan torasik aortlarının endotel-bağımlı ve -bağımsız kasılma-gevşeme yanıtlarının bozulmadan korunabildiğine dair ilk fizyolojik bulgular elde edilmiştir.

Anahtar Kelimeler:

Soğuk Depolama, Sıçan Torasik Aortu, Vasküler Düz Kas, Vazoreaktivite

Effects of Cold Storage in Krebs-Henseleit Solution at +4 °C on Vasoreactivity of the Rat Thoracic Aorta

Aim: This study aimed to investigate whether endothelium-dependent and -independent relaxation responses can be preserved intact in the rat thoracic aorta after storage for 3, 6, and 18 hours in Krebs-Henseleit solution at +4 °C. Methods: Isolated organ bath model and 10-12 weeks old male Wistar rats were used to perform the experiments. To investigate the effect of endothelium-dependent relaxation factors, the cyclooxygenase inhibitor INDO was added to the Krebs-Henseleit solution to inhibit endogenous prostanoid synthesis. Submaximal contraction response was obtained with a single dose of PE and then ACh was administered cumulatively (10-9-10-4 M) to induce endothelium-dependent relaxation responses. Besides, smooth muscle-dependent relaxation responses were obtained by applying SNP cumulatively (10-9-10-5 M) following precontraction induced by PE. The statistical significance level was considered as p0.05). Besides, cumulatively administered ACh did not cause a significant change in endothelium-dependent relaxation responses (p>0.05). Similarly, SNP did not modulate the endothelium-independent relaxation responses in aortic segments after storage for 3, 6, or 18 hours (p>0.05). Conclusion: In the present study, the first physiological findings have been obtained that the endothelium-dependent and -independent contraction-relaxation responses of rat thoracic aortas can be preserved intact after storage periods of 3, 6, or 18 hours in Krebs-Henseleit solution at +4°C.

Keywords:

Cold Storage, Rat Thoracic Aorta, Vascular Smooth Muscle, Vasoreactivity,

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