Hasan Ufuk ÇELEBİOĞLU, Ebru KÖROĞLU, Hüseyin AKŞİT, Recep TAŞ

Plantago Euphratica Bitkisinden İzole Edilen İpolamiidin Lactobacillus acidophilus ve Lactobacillus rhamnosus Bakterilerinin Probiyotik Özellikleri Üzerine Etkileri

İridoid glikozitler, doğal olarak yapraklarda, meyvelerde, tohumlarda, ağaç kabuğunda, bitki köklerinde bulunan terpenin 2- siklopentanoid türevleridir. Tıbbi olarak önemlidir, çünkü normalde bitkileri biyotik ve abiyotik ataklardan korurken birçok hastalığın tedavisinde kullanılır. Anti-mikrobiyal, anti-tümör, anti-kardiyak ve anti-enflamatuar etkilere sahiptirler. Ipolamiid, iridoid glikozitlerden biridir ve birçok bitkide doğal olarak bulunur. Ipolamiid biyolojik aktiviteleri açısından çok az bilinen bir bileşiktir. Vücuttaki faydalı mikroorganizmalar ve bitki kökenli bileşikler, insan gastrointestinal kanalında etkileşime girebilir. Probiyotikler, bağırsakların mikrobiyal dengesini geliştirerek birçok sağlık yararına sahip olan canlı mikroorganizmalardır. En bilinen ve en çok çalışılan probiyotikler arasında Lactobacillus acidophilus ve Lactobacillus rhamnosus bulunur. Bu çalışmanın amacı, ipolamiidin probiyotik bakteriler Lactobacillus rhamnosus GG (GG) ve Lactobacillus acidophilus LA-5 (LA-5) üzerindeki etkilerini araştırmaktır. Bu amaçla probiyotiklerin büyümesine farklı konsantrasyonlarda ipolamiid eklenmiş ve bakteriyel büyüme kinetiği, bakteriyel yüzey hidrofobisitesi (Solventlere Mikrobiyel Yapışma - MATS Testi) ve bakteriyel agregasyona (OtoAgregasyon Testi) etkileri incelenmiştir. Sonuçlar, ipolamidin probiyotik bakterilerin yüzey hidrofobisitesinde önemli bir değişikliğe sebep olmadığını göstermiştir. LA-5 ve GG'nin oto-agregasyon özelliklerinde doza bağlı artışlar gözlenmiştir. Bununla birlikte, ipolamiidin diğer olası biyolojik aktiviteleri hakkında bilgi edinmek için daha ayrıntılı çalışmalara ihtiyaç vardır.

Insight into Effects of Ipolamiide isolated from Plantago euphratica on Probiotic Properties of Lactobacillus acidophilus and Lactobacillus rhamnosus

Iridoid glycosides are 2-cyclopentanoid derivatives of terpene origin naturally occuring in the leaves, fruits, seeds, bark, roots of variousplants. They are medically important because used in the treatment of many diseases while normally protect plants from biotic andabiotic attacks. They have anti-microbial, anti-tumor, anti-cardiac and anti-inflammatory effects. Ipolamiide is one of the iridoidglycosides and naturally present in many plants. Ipolamiide is very little known compound in terms of the biological activities.The beneficial microorganisms in the body and the plant secondary metabolites can interact in the human gastrointestinal tract.Probiotics are live microorganisms that have many health benefits by improving microbial balance of the intestines. Among the mostknown and most studied probiotics are Lactobacillus acidophilus and Lactobacillus rhamnosus.The aim of the present study is to investigate the effects of ipolamiide on probiotic bacteria Lactobacillus rhamnosus GG (GG) andLactobacillus acidophilus LA-5 (LA-5). For this purpose, ipolamiide was added to the growth of probiotics in different concentrationsand its effects on bacterial growth kinetics, bacterial surface hydrophobicity (Microbial Adhesion to Solvents - MATS Test) and bacterialaggregation (Auto-Aggregation Test) were investigated.The results showed that ipolamiide did not show any important change in surface hydrophobicity of probiotic bacteria. Dose-dependentincreases in auto-aggregation properties of the LA-5 and GG were observed. However, further detailed studies are required to giveinsight into other possible biological activities of ipolamiide.

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