Özlem COŞKUN, Özlem ÖZTOPUZ, Başak BÜYÜK

Lentinula edodes Ekstraktının Sıçan Karaciğer Dokusu Üzerine İnflamatuar ve Apoptotik Etkisinin Değerlendirilmesi

Lentinula edodes (Shiitake mantarı) uzak Doğu ülkelerinde binlerce yıldır gıda ve ilaç olarak yetiştirilip kullanılmaktadır. Günümüzdedünya çapında en fazla tüketilen ikinci mantar türüdür. Dünya genelinde 100,000 veya daha fazla mantar türünün 100'den fazlasınıntoksik olduğu tahmin edilmektedir. Mantarlar, dünyanın pek çok yerinde değerli bir gıda maddesidir, ancak bazı türleri insanlardazararlı etkilere neden olabilir, bazı durumlarda potansiyel olarak ölümcül olabilir. Son yıllarda mantar zehirlenmesi ile ilişkili kabuledilen klinik sendromların kapsamı genişlemiştir. Shiitake mantar türünün toksik etkisi konusunda çok az araştırmaya rastlanmış olup,konuyla ilgili bilimsel araştırmalara ihtiyaç vardır. Bu mantar türü yenilebilir, ancak bazı kişilerde küçük yan etkiler veya alerjikreaksiyonlar görülebilir. Literatürde shiitakeye bağlı toksikodermi ve shiitake dermatit vakaları bildirilmiştir. Shiitake mantar sporlarıalerjik reaksiyonlara neden olduğu tespit edilmiştir. Çalışmamızın amacı sıçanlara farklı dozda uygulanan shiitake mantarınınkaraciğer üzerine toksik etkisi olup olmadığını araştırmaktır. Çalışmada sıçanlara farklı dozlarda (düşük ve yüksek dozda) uygulananShiitake mantar ekstraktının karaciğere etkisi; TNF-α (inflamatuar belirteç), Bax, Bcl-2’nin (apoptotik belirteç) ekspresyondeğişimleri incelenerek ve histopatolojik analizleri yapılarak değerlendirildi. Gruplara ait Bax, Bcl-2, TNF-α ekspresyon düzeyleriincelendiğinde Bax ekspresyon seviyesinde düşük doz ile kontrol grubu karşılaştırıldığında anlamlı bir düşüş izlendi (p

Evaluation of Inflammatory and Apoptotic Effect of Lentinula edodes Extract on Rat Liver Tissue

Lentinula edodes (Shiitake mushroom) have been grown and used as food and medicine in the Far East for thousands of years. Today, shiitake mushroom is the second most consumed mushroom species in the world and is one of the most grown edible mushrooms in the world. It is estimated that more than 100 of 100,000 or more fungal species worldwide are toxic. Mushrooms are a valuable foodstuff in many parts of the world, but some species can cause harmful effects on humans, in some cases potentially fatal. In recent years, the scope of clinical syndromes considered to be associated with fungal poisoning has expanded. Few studies have been found on the toxic effects of Shiitake mushroom species and scientific research is needed. This type of fungus is edible, but some people may experience minor side effects or allergic reactions. Toxicodermia and shiitake dermatitis cases have been reported in the literature. Shiitake mushroom spores have been found to cause allergic reactions. The aim of this study was to investigate the toxic effects of shiitake fungus administered to rats on liver. In this study, the effect of Shiitake mushroom extract applied to rats at different doses (low and high doses) on liver was evaluated by examining expression changes of TNF-α (inflammatory marker), Bax, Bcl-2 (apoptotic marker) and histopathological analysis. When the Bax, Bcl-2, TNF-α expression levels of the groups were examined, a significant decrease was observed in the Bax expression level when the low dose was compared with the control group (p

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