Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Nizamlıoğlu

MİKROSATELLİT DNA MARKÖRLERİ KULLANILARAK ATLARDA EBEVEYN TAYİNİ: BİR VAKA TAKDİMİ

At yetiþtiriciliðinde, hayvanýn gerçek deðerinin belirlenmesi, soy kütüðü kayýtlarý, bilimsel çalýþmalar ve adli olaylarda ebeveynve birey tespiti önem arz etmektedir. Bu amaçla DNA teknolojisi yaygýn olarak kullanýlmaktadýr. Sunulan bu çalýþmanýn amacý, T.C. KarsCumhuriyet Baþsavcýlýðý Hazýrlýk Bürosu tarafýndan gönderilen kýsrak at ve yavru ata ait kan ve kýl örneklerinden DNA düzeyinde ebeveyntayini ile ana-yavru iliþkisinin belirlenmesidir. Kan ve kýl örneklerinden DNA izolasyonu sonrasý, Uluslararasý Hayvan Genetiði Derneði(ISAG) tarafýndan tavsiye edilen 10 adet mikrosatellit lokusu kullanýlarak Polimeraz Zincir Reaksiyonu (PZR) yapýlmýþtýr. YükseltgenenPZR ürünleri Beckman Coulter CEQ-8000 Genetik Analiz sistemine yüklenerek kapiller elektroforez ve fragman analizi sonucunda herbir marköre ait allel ve genotipler belirlenmiþtir. Sonuçlar ortak allel yönünden deðerlendirilmiþtir. Çalýþmada Kýl2 örneði kullanýlarakyapýlan analizlerden herhangi bir PZR ürünü ve allel elde edilememiþtir. Bu örneðin detaylý incelenmesi sonucunda kýl köklerininbulunmamasý, örnekleme çalýþmasýnýn makas ile yapýldýðýný düþündürmektedir. Kýl1 ve Kan2 örneklerinde ayný allellerin gözlenmesi,bu örneklerin ayný bireye ait olduðunu göstermektedir. Kan1 ve Kan2 örnekleri AHT04, HMS02, HMS03, HTG06 ve VLH20 mikrosatellitlokuslarýnda en az birer ortak allele sahiptir. Ancak, AHT05, HMS06, HTG07 ve HTG10 lokuslarýnda ortak allellerin bulunmamasýçalýþmaya konu olan atlarda ana-yavru iliþkisinin bulunamayacaðý kanaatini oluþturmaktadýr

Anahtar Kelimeler:

At, DNA, Ebeveyn Tayini, Mikrosatellit Markör

Paternity Testing in Horses by Using Microsatellite DNA Markers: A Case Report

Parentage testing is critically important in horse breeding for actual evaluation of an animal, stud registrations, forensic studiesand scientific researches. For this reason, DNA technology is widely used. The objective of this study is to determine genetic relationshipbetween a mare and a foal by using blood and hair samples that were sent by the Attorney Generalship of Kars Province. DNA isolationswere performed by using blood and hair samples. DNA samples were amplified at 10 horse microsatellite loci which were selected froma list suggested by International Society of Animal Genetics (ISAG). The resulting polymerase chain reaction (PCR) products wereseparated by capillary electrophoresis using a Beckman Coulter CEQ- 8000 Genetic Analysis System. Allele scoring was accomplishedbased on their base-pair size by fragment analysis and genotypes were determined each loci. Genotype data were evaluated basedon common alleles. PCR products and so allele peaks were not determined for hair sample-2. When this sample was examined in detail,no hair roots were observed, which suggested that sampling was performed by a scissor. The same genotypes were observed betweenhair sample-1 and blood sample-2, therefore they belonged to the same animal. At least one common allele was observed at eachAHT04, HMS02, HMS03, HTG06 and VLH20 loci for blood samples 1 and 2. On the other hand, common alleles were not determinedfor AHT05, HMS06, HTG07 and HTG10 microsatellite loci. These results suggested that no paternity is possible between the mare andthe foal

Keywords:

Horse, DNA, Potermity testing, Microsatelit markar,

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