Marcela Capcarova, Adriana Kolesarova, Alexander V. Sirotkin

Kurşun ve molibdene maruz kalan tavuk granulusa hücrelerinin superoksid dismutaz ve antioksidan durumları

Amaç: Mevcut araştırmanın amacı in vitro kültüre edilen tavuk ovaryum granulosa hücrelerine kurşun (Pb) ve molibden (Mo) uygulaması sonrasında superoksid dismutaz (SOD) ve total antioksidan durum (TAS)’un belirlenmesidir. Gereç ve Yöntem: Ovaryum granulosa hücreleri farklı dozlarda kurşun asetat/amonyum molibdat uygulaması ile 18 saat inkübe edildi. Kurşun asetat/amonyum molibdat ise faklı dozlarda uygulandı; grup A (0.09/0.09 mg/mL), grup B (0.13/0.13 mg/mL), grup C (0.17/0.17 mg/mL), grup D (0.33/0.33 mg/mL), grup E (0.5/0.5 mg/mL) ve kontrol.Sonuçlar: Granulosa hücrelerinin antioksidan durumu Pb ve Mo dozuna bağlı olarak değişti. Her iki metalin yüksek dozu SOD aktivitesinde önemli düşmelere neden oldu. Hücrelerin TAS’u deneme gruplarında düşük belirlendi. Öneri: İz elementler hayvanların üreme sistemi üzerine doğrudan veya dolaylı olarak oksitatif strese neden olarak olumsuz etkilere neden olabilir.

Anahtar Kelimeler:

Granulosa hücreleri, kurşun, molibden, SOD, total antioksidan durum

Superoxide dismutase and antioxidant status of hens’ granulosa cells exposed to lead and molybdenum

Aim: The aim of this study was to determine the activity of superoxide dismutase (SOD) and total antioxidant status (TAS) of ovarian granulosa cells of hens cultured in vitro after lead (Pb) and molybdenum (Mo) administrations. Material and Methods: Ovarian granulosa cells were incubated with lead acetate/ammonium molybdate administrations as follows: group A (0.09/0.09 mg/mL), group B (0.13/0.13 mg/mL), group C (0.17/0.17 mg/mL), group D (0.33/0.33 mg/mL), group E (0.5/0.5 mg/mL) and the control group without any additions for 18 h. Results: Antioxidant status of granulosa cells was dependent on Pb and Mo doses. Higher doses of both metals caused significant reduction of SOD activity. TAS of cells was decreased in experimental groups. Conclusion: Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.

Keywords:

Granulosa cells, lead, molybdenum, SOD, total antioxidant status,

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