İç Anadolu Bölgesi’ndeki doğal enfekte sığırlarda solunum viruslarına karşı gelişen antikor prevalansı

Amaç: Bu çalışma İç Anadolu bölgesindeki sığırlarda major respiratorik viral ajanların seroprevalanslarının belirlenmesi amacı ile yapıldı. Gereç ve Yöntem: Bu çalışmada İç Anadolu Bölgesi’ndeki 5 ilden (Amasya, Çorum, Kayseri, Nevşehir ve Yozgat) solunum sisteminin en önemli enfeksiyöz ajanları olan Bovine Herpesvirus tip 1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus-3 (PIV-3) ve Bovine Adenovirus-3 (BAV-3)’e karşı herhangi bir aşı uygulaması yapılmamış toplam 500 sığırdan kan örnekleri toplandı. Serum örnekleri BHV-1, BVDV, BRSV, PIV-3 ve BAV-3’e karşı gelişen spesifik antikor varlıkları yönünden serolojik olarak indirekt Enzyme Linked Immunosorbent Assay (ELISA) ile incelendi.Bulgular: Serum örneklerinin seropozitivite oranları BHV1, BVDV, BRSV, PIV-3 ve BAV-3 için sırasıyla %57.8, %68.0, %78.2, % 85.6 ve %76.8 olarak belirlendi. Öneri: Sonuç olarak İç Anadolu Bölgesi’ndeki sığırlarda solunum sistemi hastalıklarında temel enfeksiyöz viral ajanların varlığının devam ettiği ve aşılama programlarının uygulanması gerektiği ifade edilebilir.

Anahtar Kelimeler:

BHV-1, BVDV, BRSV, PIV-3, BAV-3

Antibody prevalence against respiratory viruses in naturally infected cattle in Central Anatolia

Aim: The aim of this study was to determination of the seroprevalences of major respiratoric viral agents of cattle in central region Anatolia. Materials and Methods: In the present study, blood serum samples were collected from 500 cattle not vaccinated against Bovine Herpesvirus type 1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza virus-3 (PIV-3) and Bovine Adenovirus-3 (BAV-3), which are the most important infectious agents of respiratory system in cattle, in five provinces (Amasya, Çorum, Kayseri, Nevşehir, and Yozgat) of Central Anatolia. Serum samples were investigated serologically with indirect Enzyme Linked Immunosorbent Assay (ELISA) for specific antibodies developed against BHV-1, BVDV, BRSV, PIV-3 and BAV-3.Results: Seropositivity of the serum samples of BHV-1, BVDV, BRSV, PIV-3 and BAV-3 were determined as 57.8%, 68.0%, 78.2%, 85.6% and 76.8%, respectively.Conclusions: It may be stated that main infectious viral agents for respiratory disease of cattle in Central Anatolia is still presents, and vaccine programs should be applied.

Keywords:

BHV-1, BVDV, BRSV, PIV-3, BAV-3,

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Akca Y, 1981. Serological investigations of infectious bovine rhinotracheitis-infectious pustular vulvovaginitis in cattle and sheep in Turkey, PhD Thesis, Ankara University, Ankara.
Algirdas S, Eugenijus J, Kestaitiene K, Petkevicius S, Lukauskas K, Liutkeviciene V, Milius J, Venskutonis D, Mockeliunas R, Jokimas J, 2008. Distribution of economically important viral diseases in cattle. Vet Med Zoot, 41, 63.
Alkan F, Ozkul A, Karaoglu T, Bilge S, Akca Y, Burgu I, Yesilbag K, Oguzoglu C, 1997. Seroepidemiological studies on respiratory infections of cattle caused by viruses. Vet J Ankara Univ, 44, 73-80.
Alkan F, Ozkul A, Bilge-Dagalp S, Yesilbag K, Oguzoglu TC, Akca Y, Burgu I, 2000. Virological and serological studies on the role of PI-3 Virus, BRSV, BVDV and BHV-1 on respiratory infections of cattle. I. The detection of etiological agents by direct immunofluorescence technique. Dtsch Ttierarztl Wschr, 107, 193-195.
Antonis AFG, de Jong MC, van der Poel WHM, van der Most RG, Stockhofe-Zurwieden N, Kimman T, Schrijver RS, 2010. Age-dependent differences in the pathogenesis of bovine respiratory syncytial virus infections related to the development of natural immunocompetence. J Gen Virol, 91, 2497-2506.
Beaudeau F, Bjorkman C, Alenius S, Frossling J, 2010. Spatial patterns of bovine corona virus and bovine respiratory syncytial virus in the Swedish beef cattle population. Acta Vet Scand, 52, 33.
Bilge S, 1998. Detection of antibodies of IBR-IPV infection in blood and milk sera by serum neutralisation test and virus isolation from milk samples dairy cows. Vet J Ankara Univ, 45, 313-321.
Burgu I, Akca Y, 1982. Serologische untersuchungen gegen einige virusinfektionen bei Rindern in der staatlichen Züchtfarm Gelemen. Vet J Ankara Univ, 29, 506-512.
Cabalar M, 1994. The isolation and seroepidemiological studies of IBR/IPV virus on cows with fertility problems. Vet J Ankara Univ, 41, 337-349.
Davison AJ, Benko M, Harrach B, 2003. Genetic content and evolution of adenoviruses. J Gen Virol, 84, 2895-2908.
Duman R, Yavru S, Kale M, Avci O, 2009. Seroprevalence of viral upper respiratory infections in dairy cattle. Kafkas Univ Vet Fak Derg, 15, 539-542.
Gencay A, Dagalp SB, Sahna KC, Pinar D, Basaran Z, 2009. The seroprevalence of bovine herpes virus type-1 (BHV-1) infection in cattle in Kayseri region. Firat Univ Vet J Health Sci, 23, 47-52.
Grondahl C, Uttenthal A, Houe H, Rasmussen TB, Hoyer MJ, Larsen LE, 2003. Characterisation of a pestivirus isolated from persistently infected mousedeer (Tragulus javanicus). Arch Virol, 148, 1455-1463.
Gumusova SO, Yazıcı Z, Albayrak H, Cakiroglu D, 2007. Seroprevalence of bovine viral respiratory diseases. Acta Vet (Beograd), 57, 11-16.
Gurturk S, Finci E, Burgu I, 1974. The research on Infectious Bovine Rhinotracheitis in Turkey. Vet J Ankara Univ, 21, 34-46.
Heckert RA, Saif LJ, Hoblet KH, Agnes AG, 1990. A longitudinal study of bovine coronavirus enteric and respiratory infections in dairy calves in two herds in Ohio. Vet Microbiol, 22, 187-201.
Klem TB, Rimstad E, Stokstad M, 2014. Occurrence and phylogenetic analysis of bovine respiratory syncytial virus in outbreaks of respiratory disease in Norway. BMC Vet Res, 10, 15. DOI: 10.1186/1746-6148-10-15.
Larsen LE, 2000. Bovine Respiratory Syncytial Virus (BRSV): A review. Acta Vet Scand, 41, 1-21.
Larsen LE, Tegtmeier C, Pedersen E, 2001. Bovine Respiratory Syncytial Virus (BRSV) pneumonia in beef calf herds despite vaccination. Acta Vet Scand, 42, 113-121.
Mars MH, Bruschke CJ, van Oirschot JT, 1999. Airborne transmission of BHV1, BRSV, and BVDV among cattle is possible under experimental conditions. Vet Microbiol, 66, 197- 207.
Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD, 1995. Virus taxonomy: sixth report of the International Committee on Taxonomy of Viruses. Springer-Verlag, Vienna, Austria.
Nandi S, Kumar M, Manohar M, Chauhan RS, 2009. Bovine herpes virus infections in cattle. Anim Health Res Rev, 10, 85-98.
Pardon B, De Bleecker K, Dewulf J, Callens J, Boyen F, Catry B, Deprez P, 2011. Prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. Vet Rec, 169, 278.
Passler T, Ditchkoff SS, Givens MD, Brock KV, Deyoung RW, Walz PH, 2010. Transmission of bovine viral diarrhea virus among white-tailed deer (Odocoileus virginianus). Vet Res, 41, 20.
Potgieter LND, McCracken MD, Hopkins FM, Walker RD, 1984. Effect of bovine viral diarrhea virus infection on the distribution of infectious bovine rhinotracheitis virus in calves. Am J Vet Res, 45, 687-690.
Radostits OM, Gay CC, Blood DC, Hinchccliff KW, 2000. Veterinary medicine textbook of the diseases of cattle, sheep, pigs, goats and horses. Ed; Saunders WB, 9. ed., Company, London, pp; 1160-1172.
Rajkhowa S, Rajkhowa C, Rahman H, Bujarbaruah KM, 2004. Seroprevalence of infectious bovine rhinotracheitis in Mithun in India. Rev Sci Tech, 23, 821-829.
Roshtkhari F, Mohammadi G, Mayameei A, 2012. Serological evaluation of relationship between viral pathogens (BHV- 1, BVDV, BRSV, PI-3V, and Adeno 3) and dairy calf pneumonia by indirect ELISA. Trop Anim Health Prod, 44, 1105- 1110.
Sakhaee EA, Khalili M, Kazeminia S, 2009. Serological study of bovine viral respiratory diseases in dairy cattle of Kerman (Iran). Iran J Vet Res, 10, 49-53.
Shirvani E, Lotfi M, Kamalzadeh M, Noaman V, Bahriari M, Morovati H, Hatami A, 2012. Seroepidemiological study of bovine respiratory viruses (BRSV, BoHV-1, PI-3V, BVDV, and BAV-3) in dairy cattle in central region of Iran (Esfahan province). Trop Anim Health Prod, 44, 191-195.
Snowder GD, Van Vleck LD, Cundiff LV, Bennett GL, 2006. Bovine respiratory disease in feedlot cattle: Environmental, genetic, and economic factors. J Anim Sci, 84, 1999-2008.
Thomson DU, White BJ, 2006. Back grounding beef cattle. Vet Clin North Am Food Anim Pract, 22, 373-398.
Viuff B, Tjornehoj K, Larsen LE, Rontved CM, Uttenthal A, Ronsholt L, Alexandersen S, 2002. Replication and clearance of respiratory syncytial virus. Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus. Am J Pathol, 161, 2195-2207.
Yavru S, Simsek A, Yapkic O, Kale M, 2005. Serological evaluation of viral infections in bovine respiratory tract. Acta Vet (Beogard), 2, 219-226.
Yesilbag K, Gungor B, 2008. Seroprevalence of bovine respiratory viruses in North-Western Turkey. Trop Anim Health Pro, 40, 55-60.
Zakhartchouk AN, Pyne C, Mutwiri GK, Papp Z, BacaEstrada ME, Griebel P, Babiuk LA, Tikoo SK, 1999. Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1. J Gen Virol, 80, 1263-1269.