Filipinler, Cebu’dan elde edilen Rhipicephalus sanguineus kenelerinden Coxiella spp. türlerinin moleküler olarak ilk belirlenmesi

Coxiella türleri kene ısırması ile geçebilen patojenik etkendir. Mevcut araştırma Filipinler, Cebu’da Rhipicephalus sanguineus kenelerinden Coxielle türlerinin varlığını moleküler tekniklerle belirlemek için yapıldı. Otuzaltı köpekten elde edilen toplam 164 adet kene Nested Polimeraz Zincir Reaksiyonu (PCR) ile kısmi 16S ribozomal RNA gen bölgesi çoğaltılarak analiz edildi. Beş adet köpekten elde edilen 9 adet kene pozitif (%5.5) bulundu. Sekans analizi sonucunda Coxiella burnetii (%96.3) ve Coxiella sp. endosymbiont (%98.1-100) ile benzer Coxiella spp.'ya ait DNA fragmentleri tespit edildi. Belirlenen DNA fragmentleri birbirleri ile de %98.3-100 oranında benzerdi. Bu çalışma Filipinler Cebu'da Rhipicephalus sanguineus kenelerinden elde edilen Coxielle spp. varlığı- nı gösteren ilk araştırmadır.

Anahtar Kelimeler:

Coxiella sp., Rhipicephalus sanguineus, Filipinler

First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines

Coxiella species are known to be potentially pathogenic tickborne organisms. This study was aimed to investigate the presence of Coxiella sp. in Rhipicephalus sanguineus ticks in Cebu, Philippines by molecular based techniques. A total of 164 ticks were collected from 36 dogs and analyzed by a Nested Polymerase Chain Reaction (PCR) for the amplification of partial 16S ribosomal RNA gene region. Nine ticks (5.5%) obtained from 5 dogs were found positive. Sequencing revealed partial DNA fragments of Coxiella spp. which were 96.3% and 98.1-100% identical to Coxiella burnetii and Coxiella sp. endosymbiont, respectively. The detected DNA fragments also shared 98.3-100% identities with each other. This study is the first report on the existence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines.

Keywords:

Coxiella sp., Rhipicephalus sanguineus, Philippines,

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